{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T22:19:38Z","timestamp":1789597178221,"version":"build-2803163510"},"reference-count":19,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2018,7,31]],"date-time":"2018-07-31T00:00:00Z","timestamp":1532995200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2018,7,31]],"date-time":"2018-07-31T00:00:00Z","timestamp":1532995200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2018,7,31]],"date-time":"2018-07-31T00:00:00Z","timestamp":1532995200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["1002748"],"award-info":[{"award-number":["1002748"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"publisher","award":["DP160103100"],"award-info":[{"award-number":["DP160103100"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000854","name":"Human Frontier Science Program","doi-asserted-by":"publisher","award":["RGP0039\/2011"],"award-info":[{"award-number":["RGP0039\/2011"]}],"id":[{"id":"10.13039\/501100000854","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["1063696"],"award-info":[{"award-number":["1063696"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Branching morphogenesis of the ureteric bud is integral to kidney development; establishing the collecting ducts of the adult organ and driving organ expansion via peripheral interactions with nephron progenitor cells. A recent study suggested that termination of tip branching within the developing kidney involved stochastic exhaustion in response to nephron formation, with such a termination event representing a unifying developmental process evident in many organs. To examine this possibility, we have profiled the impact of nephron formation and maturation on elaboration of the ureteric bud during mouse kidney development. We find a distinct absence of random branch termination events within the kidney or evidence that nephrogenesis impacts the branching program or cell proliferation in either tip or progenitor cell niches. Instead, organogenesis proceeds in a manner indifferent to the development of these structures. Hence, stochastic cessation of branching is not a unifying developmental feature in all branching organs.<\/jats:p>","DOI":"10.7554\/elife.38992","type":"journal-article","created":{"date-parts":[[2018,7,31]],"date-time":"2018-07-31T08:01:32Z","timestamp":1533024092000},"source":"Crossref","is-referenced-by-count":28,"title":["Branching morphogenesis in the developing kidney is not impacted by nephron formation or integration"],"prefix":"10.7554","volume":"7","author":[{"given":"Kieran M","family":"Short","sequence":"first","affiliation":[{"name":"Department of Anatomy and Developmental Biology, Monash Biomedicine Discovery Institute, Monash University, Melbourne, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander N","family":"Combes","sequence":"additional","affiliation":[{"name":"Murdoch Children's Research Institute, Parkville, Australia"},{"name":"Department of Anatomy and Neuroscience, School of Biomedical Sciences, University of Melbourne, Parkville, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valerie","family":"Lisnyak","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Developmental Biology, Monash Biomedicine Discovery Institute, Monash University, Melbourne, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James G","family":"Lefevre","sequence":"additional","affiliation":[{"name":"Division of Genomics of Development and Disease, Institute for Molecular Bioscience, The University of Queensland, Brisbane, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lynelle K","family":"Jones","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Developmental Biology, Monash Biomedicine Discovery Institute, Monash University, Melbourne, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa H","family":"Little","sequence":"additional","affiliation":[{"name":"Murdoch Children's Research Institute, Parkville, Australia"},{"name":"Department of Anatomy and Neuroscience, School of Biomedical Sciences, University of Melbourne, Parkville, Australia"},{"name":"Department of Pediatrics, Faculty of Medicine, Dentistry and Health Sciences, University of Melbourne, Parkville, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas A","family":"Hamilton","sequence":"additional","affiliation":[{"name":"Division of Genomics of Development and Disease, Institute for Molecular Bioscience, The University of Queensland, Brisbane, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1727-7829","authenticated-orcid":true,"given":"Ian M","family":"Smyth","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Developmental Biology, Monash Biomedicine Discovery Institute, Monash University, Melbourne, Australia"},{"name":"Department of Biochemistry and Molecular Biology, Monash Biomedicine Discovery Institute, Monash University, Melbourne, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4374","published-online":{"date-parts":[[2018,7,31]]},"reference":[{"key":"bib1","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1016\/j.devcel.2015.09.009","article-title":"Intrinsic Age-Dependent changes and Cell-Cell contacts regulate nephron progenitor lifespan","volume":"35","author":"Chen","year":"2015","journal-title":"Developmental Cell"},{"key":"bib2","doi-asserted-by":"publisher","first-page":"297","DOI":"10.1016\/j.ydbio.2016.06.028","article-title":"Cap mesenchyme cell swarming during kidney development is influenced by attraction, repulsion, and adhesion to the ureteric tip","volume":"418","author":"Combes","year":"2016","journal-title":"Developmental Biology"},{"key":"bib3","doi-asserted-by":"publisher","first-page":"2859","DOI":"10.1038\/nprot.2014.193","article-title":"An integrated pipeline for the multidimensional analysis of branching morphogenesis","volume":"9","author":"Combes","year":"2014","journal-title":"Nature Protocols"},{"key":"bib4","doi-asserted-by":"publisher","first-page":"273","DOI":"10.1016\/j.ydbio.2009.05.578","article-title":"Analysis of early nephron patterning reveals a role for distal RV proliferation in fusion to the ureteric tip via a cap mesenchyme-derived connecting segment","volume":"332","author":"Georgas","year":"2009","journal-title":"Developmental Biology"},{"key":"bib5","doi-asserted-by":"publisher","first-page":"242","DOI":"10.1016\/j.cell.2017.08.026","article-title":"A unifying theory of branching morphogenesis","volume":"171","author":"Hannezo","year":"2017","journal-title":"Cell"},{"key":"bib6","doi-asserted-by":"publisher","first-page":"1682","DOI":"10.1681\/ASN.2012030283","article-title":"Invasion of distal nephron precursors associates with tubular interconnection during nephrogenesis","volume":"23","author":"Kao","year":"2012","journal-title":"Journal of the American Society of Nephrology"},{"key":"bib7","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1016\/j.stem.2008.05.020","article-title":"Six2 defines and regulates a multipotent self-renewing nephron progenitor population throughout mammalian kidney development","volume":"3","author":"Kobayashi","year":"2008","journal-title":"Cell Stem Cell"},{"key":"bib8","doi-asserted-by":"publisher","first-page":"85060X","DOI":"10.1117\/12.935640","article-title":"Drishti: a volume exploration and presentation tool","volume-title":"Proceedings Volume 8506: Developments in X-Ray Tomography VIII","author":"Limaye","year":"2012","unstructured":"Limaye A. 2012. Drishti: a volume exploration and presentation tool. Proceedings Volume 8506: Developments in X-Ray Tomography VIII. SPIE Optical Engineering + Applications."},{"key":"bib9","doi-asserted-by":"publisher","first-page":"e04000","DOI":"10.7554\/eLife.04000","article-title":"Integrated \u03b2-catenin, BMP, PTEN, and Notch signalling patterns the nephron","volume":"3","author":"Lindstr\u00f6m","year":"2015","journal-title":"eLife"},{"key":"bib10","doi-asserted-by":"publisher","first-page":"273","DOI":"10.1016\/S0140-6736(13)60311-6","article-title":"Effect of fetal and child health on kidney development and long-term risk of hypertension and kidney disease","volume":"382","author":"Luyckx","year":"2013","journal-title":"The Lancet"},{"key":"bib11","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1016\/j.devcel.2009.12.010","article-title":"Preparing for the first breath: genetic and cellular mechanisms in lung development","volume":"18","author":"Morrisey","year":"2010","journal-title":"Developmental Cell"},{"key":"bib12","doi-asserted-by":"publisher","first-page":"1113","DOI":"10.1242\/dev.142950","article-title":"Novel fixed z-direction (FiZD) kidney primordia and an organoid culture system for time-lapse confocal imaging","volume":"144","author":"Saarela","year":"2017","journal-title":"Development"},{"key":"bib13","doi-asserted-by":"publisher","first-page":"313","DOI":"10.1038\/nature21046","article-title":"Identity and dynamics of mammary stem cells during branching morphogenesis","volume":"542","author":"Scheele","year":"2017","journal-title":"Nature"},{"key":"bib14","doi-asserted-by":"publisher","first-page":"471","DOI":"10.1242\/dev.088500","article-title":"Spatial mapping and quantification of developmental branching morphogenesis","volume":"140","author":"Short","year":"2013","journal-title":"Development"},{"key":"bib15","doi-asserted-by":"publisher","first-page":"188","DOI":"10.1016\/j.devcel.2014.02.017","article-title":"Global quantification of tissue dynamics in the developing mouse kidney","volume":"29","author":"Short","year":"2014","journal-title":"Developmental Cell"},{"key":"bib16","doi-asserted-by":"publisher","first-page":"1132","DOI":"10.1038\/ki.2010.42","article-title":"Tomographic quantification of branching morphogenesis and renal development","volume":"77","author":"Short","year":"2010","journal-title":"Kidney International"},{"key":"bib17","doi-asserted-by":"publisher","first-page":"241","DOI":"10.1002\/(SICI)1520-6408(1999)24:3\/4<241::AID-DVG7>3.0.CO;2-R","article-title":"Expression of green fluorescent protein in the ureteric bud of transgenic mice: a new tool for the analysis of ureteric bud morphogenesis","volume":"24","author":"Srinivas","year":"1999","journal-title":"Developmental Genetics"},{"key":"bib18","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1002\/jez.1402490109","article-title":"A staging system for mouse limb development","volume":"249","author":"Wanek","year":"1989","journal-title":"Journal of Experimental Zoology"},{"key":"bib19","doi-asserted-by":"publisher","first-page":"98","DOI":"10.1016\/j.ydbio.2004.03.025","article-title":"Real-time analysis of ureteric bud branching morphogenesis in vitro","volume":"271","author":"Watanabe","year":"2004","journal-title":"Developmental Biology"}],"container-title":["eLife"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/cdn.elifesciences.org\/articles\/38992\/elife-38992-v2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/cdn.elifesciences.org\/articles\/38992\/elife-38992-v2.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/elifesciences.org\/articles\/38992","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T20:59:50Z","timestamp":1697057990000},"score":1,"resource":{"primary":{"URL":"https:\/\/elifesciences.org\/articles\/38992"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,7,31]]},"references-count":19,"alternative-id":["10.7554\/eLife.38992"],"URL":"https:\/\/doi.org\/10.7554\/elife.38992","archive":["CLOCKSS"],"relation":{"has-review":[{"id-type":"doi","id":"10.7554\/eLife.38992.017","asserted-by":"object"},{"id-type":"doi","id":"10.7554\/eLife.38992.016","asserted-by":"object"}]},"ISSN":["2050-084X"],"issn-type":[{"value":"2050-084X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,7,31]]},"article-number":"e38992"}}