{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T16:13:27Z","timestamp":1791994407823,"version":"4.4.0"},"reference-count":39,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2019,4,30]],"date-time":"2019-04-30T00:00:00Z","timestamp":1556582400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,4,30]],"date-time":"2019-04-30T00:00:00Z","timestamp":1556582400000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,4,30]],"date-time":"2019-04-30T00:00:00Z","timestamp":1556582400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Australian National Health and Medical Research Council","award":["APP1145121"],"award-info":[{"award-number":["APP1145121"]}]},{"DOI":"10.13039\/501100001108","name":"The Ophthalmic Research Institute of Australia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001108","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["EY026030"],"award-info":[{"award-number":["EY026030"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Lowy Medical Research Institute"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>The human macula is more susceptible than the peripheral retina to developing blinding conditions such as age-related macular degeneration, diabetic retinopathy. A key difference between them may be the nature of their M\u00fcller cells. We found primary cultured M\u00fcller cells from macula and peripheral retina display significant morphological and transcriptomic differences. Macular M\u00fcller cells expressed more phosphoglycerate dehydrogenase (PHGDH, a rate-limiting enzyme in serine synthesis) than peripheral M\u00fcller cells. The serine synthesis, glycolytic and mitochondrial function were more activated in macular than peripheral M\u00fcller cells. Serine biosynthesis is critical in defending against oxidative stress. Intracellular reactive oxygen species and glutathione levels were increased in primary cultured macular M\u00fcller cells which were more susceptible to oxidative stress after inhibition of PHGDH. Our findings indicate serine biosynthesis is a critical part of the macular defence against oxidative stress and suggest dysregulation of this pathway as a potential cause of macular pathology.<\/jats:p>","DOI":"10.7554\/elife.43598","type":"journal-article","created":{"date-parts":[[2019,4,29]],"date-time":"2019-04-29T20:15:57Z","timestamp":1556568957000},"source":"Crossref","is-referenced-by-count":55,"title":["Human macular M\u00fcller cells rely more on serine biosynthesis to combat oxidative stress than those from the periphery"],"prefix":"10.7554","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8074-8999","authenticated-orcid":true,"given":"Ting","family":"Zhang","sequence":"first","affiliation":[{"name":"Save Sight Institute, Sydney Medical School, Faculty of Medicine and Health, The University of Sydney, Sydney, 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