{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,6]],"date-time":"2026-11-06T22:47:15Z","timestamp":1794005235608,"version":"4.8.0"},"reference-count":96,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2019,3,13]],"date-time":"2019-03-13T00:00:00Z","timestamp":1552435200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,3,13]],"date-time":"2019-03-13T00:00:00Z","timestamp":1552435200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,3,13]],"date-time":"2019-03-13T00:00:00Z","timestamp":1552435200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008494","name":"Vontobel-Stiftung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008494","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004337","name":"Roche","doi-asserted-by":"publisher","award":["RPF 378"],"award-info":[{"award-number":["RPF 378"]}],"id":[{"id":"10.13039\/100004337","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","award":["31003A_173008"],"award-info":[{"award-number":["31003A_173008"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elifesciences.org"],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>\n                    Age-related macular degeneration (AMD) is a progressive disease of the retinal pigment epithelium (RPE) and the retina leading to loss of central vision. Polymorphisms in genes involved in lipid metabolism, including the ATP-binding cassette transporter A1 (\n                    <jats:italic>ABCA1<\/jats:italic>\n                    ), have been associated with AMD risk. However, the significance of retinal lipid handling for AMD pathogenesis remains elusive. Here, we study the contribution of lipid efflux in the RPE by generating a mouse model lacking ABCA1 and its partner ABCG1 specifically in this layer. Mutant mice show lipid accumulation in the RPE, reduced RPE and retinal function, retinal inflammation and RPE\/photoreceptor degeneration. Data from human cell lines indicate that the\n                    <jats:italic>ABCA1<\/jats:italic>\n                    AMD risk-conferring allele decreases\n                    <jats:italic>ABCA1<\/jats:italic>\n                    expression, identifying the potential molecular cause that underlies the genetic risk for AMD. Our results highlight the essential homeostatic role for lipid efflux in the RPE and suggest a pathogenic contribution of reduced ABCA1 function to AMD.\n                  <\/jats:p>","DOI":"10.7554\/elife.45100","type":"journal-article","created":{"date-parts":[[2019,3,13]],"date-time":"2019-03-13T09:00:16Z","timestamp":1552467616000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.45100","source":"Crossref","is-referenced-by-count":100,"title":["Impaired ABCA1\/ABCG1-mediated lipid efflux in the mouse retinal pigment epithelium (RPE) leads to retinal degeneration"],"prefix":"10.7554","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5764-1656","authenticated-orcid":true,"given":"Federica","family":"Storti","sequence":"first","affiliation":[{"name":"Lab for Retinal Cell Biology, Department of Ophthalmology, University of Zurich, Schlieren, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katrin","family":"Klee","sequence":"additional","affiliation":[{"name":"Lab for Retinal Cell Biology, Department of Ophthalmology, University of Zurich, Schlieren, Switzerland"},{"name":"Center for Integrative Human Physiology, University of Zurich, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vyara","family":"Todorova","sequence":"additional","affiliation":[{"name":"Lab for Retinal Cell Biology, Department of Ophthalmology, University of Zurich, Schlieren, Switzerland"},{"name":"Neuroscience Center Zurich, University of Zurich, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Regula","family":"Steiner","sequence":"additional","affiliation":[{"name":"Institute of Clinical Chemistry, University of Zurich, Schlieren, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alaa","family":"Othman","sequence":"additional","affiliation":[{"name":"Institute of Clinical Chemistry, University of Zurich, Schlieren, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saskia","family":"van der Velde-Visser","sequence":"additional","affiliation":[{"name":"Department of Human Genetics, Radboud University Medical Center, Nijmegen, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marijana","family":"Samardzija","sequence":"additional","affiliation":[{"name":"Lab for Retinal Cell Biology, Department of Ophthalmology, University of Zurich, Schlieren, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Meneau","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, University Hospital Zurich, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maya","family":"Barben","sequence":"additional","affiliation":[{"name":"Lab for Retinal Cell Biology, Department of Ophthalmology, University of Zurich, Schlieren, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duygu","family":"Karademir","sequence":"additional","affiliation":[{"name":"Lab for Retinal Cell Biology, Department of Ophthalmology, University of Zurich, Schlieren, Switzerland"},{"name":"Center for Integrative Human Physiology, University of Zurich, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valda","family":"Pauzuolyte","sequence":"additional","affiliation":[{"name":"Lab for Retinal Cell Biology, Department of Ophthalmology, University of Zurich, Schlieren, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8803-9369","authenticated-orcid":true,"given":"Sanford L","family":"Boye","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, University of Florida, Gainesville, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Blaser","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, University Hospital Zurich, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christoph","family":"Ullmer","sequence":"additional","affiliation":[{"name":"Roche Pharma Research and Early Development, Roche Innovation Center Basel, F Hoffmann-La Roche Ltd., Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua L","family":"Dunaief","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Scheie Eye Institute, University of Pennsylvania, Philadelphia, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thorsten","family":"Hornemann","sequence":"additional","affiliation":[{"name":"Institute of Clinical Chemistry, University of Zurich, Schlieren, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucia","family":"Rohrer","sequence":"additional","affiliation":[{"name":"Institute of Clinical Chemistry, University of Zurich, Schlieren, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anneke","family":"den Hollander","sequence":"additional","affiliation":[{"name":"Department of Human Genetics, Radboud University Medical Center, Nijmegen, Netherlands"},{"name":"Department of Ophthalmology, Radboud University Medical Center, Nijmegen, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arnold","family":"von Eckardstein","sequence":"additional","affiliation":[{"name":"Institute of Clinical Chemistry, University of Zurich, Schlieren, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00fcrgen","family":"Fingerle","sequence":"additional","affiliation":[{"name":"Natural and Medical Sciences Institute, University of T\u00fcbingen, T\u00fcbingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cyrille","family":"Maugeais","sequence":"additional","affiliation":[{"name":"Roche Pharma Research and Early Development, Roche Innovation Center Basel, F Hoffmann-La Roche Ltd., Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9318-4352","authenticated-orcid":true,"given":"Christian","family":"Grimm","sequence":"additional","affiliation":[{"name":"Lab for Retinal Cell Biology, Department of Ophthalmology, University of Zurich, Schlieren, Switzerland"},{"name":"Center for Integrative Human Physiology, University of Zurich, Zurich, Switzerland"},{"name":"Neuroscience Center Zurich, University of Zurich, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4374","published-online":{"date-parts":[[2019,3,13]]},"reference":[{"key":"bib1","doi-asserted-by":"publisher","DOI":"10.1038\/nature11632","article-title":"An integrated map of genetic variation from 1,092 human genomes","volume":"491","author":"Abecasis","year":"2012","journal-title":"Nature"},{"key":"bib2","doi-asserted-by":"publisher","first-page":"603","DOI":"10.1016\/j.bbadis.2014.01.010","article-title":"Regulation of the cholesterol efflux transporters ABCA1 and ABCG1 in retina in hemochromatosis and by the endogenous siderophore 2,5-dihydroxybenzoic acid","volume":"1842","author":"Ananth","year":"2014","journal-title":"Biochimica Et Biophysica Acta (BBA) - Molecular Basis of Disease"},{"key":"bib3","doi-asserted-by":"publisher","first-page":"1765","DOI":"10.1016\/j.bbadis.2015.05.015","article-title":"Recent insights on the role of cholesterol in non-alcoholic fatty liver disease","volume":"1852","author":"Arguello","year":"2015","journal-title":"Biochimica Et Biophysica Acta (BBA) - Molecular Basis of Disease"},{"key":"bib4","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.120824","article-title":"Impaired monocyte cholesterol clearance initiates age-related retinal degeneration and vision loss","volume":"3","author":"Ban","year":"2018","journal-title":"JCI Insight"},{"key":"bib5","doi-asserted-by":"publisher","first-page":"1414","DOI":"10.1194\/jlr.M084442","article-title":"Disrupted cholesterol metabolism promotes age-related photoreceptor neurodegeneration","volume":"59","author":"Ban","year":"2018","journal-title":"Journal of Lipid Research"},{"key":"bib6","doi-asserted-by":"publisher","first-page":"2071","DOI":"10.1038\/s41418-018-0094-7","article-title":"Hif1a inactivation rescues photoreceptor degeneration induced by a chronic hypoxia-like stress","volume":"25","author":"Barben","year":"2018","journal-title":"Cell Death & Differentiation"},{"key":"bib7","doi-asserted-by":"publisher","DOI":"10.1186\/s13024-018-0243-y","article-title":"Targeting Hif1a rescues cone degeneration and prevents subretinal neovascularization in a model of chronic hypoxia","volume":"13","author":"Barben","year":"2018","journal-title":"Molecular Neurodegeneration"},{"key":"bib8","doi-asserted-by":"publisher","first-page":"367","DOI":"10.1016\/S0039-6257(05)80092-X","article-title":"An international classification and grading system for age-related maculopathy and age-related macular degeneration. the international ARM epidemiological study group","volume":"39","author":"Bird","year":"1995","journal-title":"Survey of Ophthalmology"},{"key":"bib9","doi-asserted-by":"publisher","first-page":"4327","DOI":"10.1093\/hmg\/ddx319","article-title":"Retinal pigment epithelium cholesterol efflux mediated by the 18\u2009kDa translocator protein, TSPO, a potential target for treating age-related macular degeneration","volume":"26","author":"Biswas","year":"2017","journal-title":"Human Molecular Genetics"},{"key":"bib10","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1007\/978-1-4939-8669-9_6","volume-title":"Retinal Degeneration: Methods and Protocols","author":"Brandl","year":"2019"},{"key":"bib11","doi-asserted-by":"publisher","first-page":"521","DOI":"10.1016\/j.exer.2008.08.010","article-title":"Lipid and fatty acid profile of the retina, retinal pigment epithelium\/choroid, and the lacrimal gland, and associations with adipose tissue fatty acids in human subjects","volume":"87","author":"Bretillon","year":"2008","journal-title":"Experimental Eye Research"},{"key":"bib12","doi-asserted-by":"publisher","first-page":"655","DOI":"10.1016\/j.bbalip.2006.04.012","article-title":"Lipid efflux by the ATP-binding cassette transporters ABCA1 and ABCG1","volume":"1761","author":"Cavelier","year":"2006","journal-title":"Biochimica Et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids"},{"key":"bib13","doi-asserted-by":"publisher","DOI":"10.1186\/s12931-017-0526-9","article-title":"Examining the role of ABC lipid transporters in pulmonary lipid homeostasis and inflammation","volume":"18","author":"Chai","year":"2017","journal-title":"Respiratory Research"},{"key":"bib14","doi-asserted-by":"publisher","first-page":"7401","DOI":"10.1073\/pnas.0912702107","article-title":"Genetic variants near TIMP3 and high-density lipoprotein-associated loci influence susceptibility to age-related macular degeneration","volume":"107","author":"Chen","year":"2010","journal-title":"PNAS"},{"key":"bib15","doi-asserted-by":"publisher","first-page":"12197","DOI":"10.1074\/jbc.M109.096933","article-title":"Targeted deletion of hepatocyte ABCA1 leads to very low density lipoprotein triglyceride overproduction and low density lipoprotein hypercatabolism","volume":"285","author":"Chung","year":"2010","journal-title":"Journal of Biological Chemistry"},{"key":"bib16","doi-asserted-by":"publisher","first-page":"284","DOI":"10.1186\/1479-7364-4-5-284","article-title":"Functional intronic polymorphisms: buried treasure awaiting discovery within our genes","volume":"4","author":"Cooper","year":"2010","journal-title":"Human Genomics"},{"key":"bib17","doi-asserted-by":"publisher","first-page":"1638","DOI":"10.1136\/bjophthalmol-2011-300344","article-title":"The oil spill in ageing bruch membrane","volume":"95","author":"Curcio","year":"2011","journal-title":"British Journal of Ophthalmology"},{"key":"bib18","doi-asserted-by":"publisher","first-page":"1116","DOI":"10.1136\/bjo.2008.144006","article-title":"Expression of reverse cholesterol transport proteins ATP-binding cassette A1 (ABCA1) and scavenger receptor BI (SR-BI) in the retina and retinal pigment epithelium","volume":"93","author":"Duncan","year":"2009","journal-title":"British Journal of Ophthalmology"},{"key":"bib19","doi-asserted-by":"publisher","first-page":"120","DOI":"10.1016\/j.exer.2017.10.015","article-title":"Conditional loss of Spata7 in photoreceptors causes progressive retinal degeneration in mice","volume":"166","author":"Eblimit","year":"2018","journal-title":"Experimental Eye Research"},{"key":"bib20","doi-asserted-by":"publisher","first-page":"5525","DOI":"10.1167\/iovs.10-6827","article-title":"Evaluation of Serum Lipid Concentrations and Genetic Variants at High-Density Lipoprotein Metabolism Loci and TIMP3 in Age-Related Macular Degeneration","volume":"52","author":"Fauser","year":"2011","journal-title":"Investigative Opthalmology & Visual Science"},{"key":"bib21","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1007\/978-3-319-09665-0_4","volume-title":"High Density Lipoproteins: From Biological Understanding to Clinical Exploitation","author":"Favari","year":"2015"},{"key":"bib22","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1016\/0163-7827(83)90004-8","article-title":"Chemistry and metabolism of lipids in the vertebrate retina","volume":"22","author":"Fliesler","year":"1983","journal-title":"Progress in Lipid Research"},{"key":"bib23","doi-asserted-by":"publisher","first-page":"3399","DOI":"10.1194\/jlr.R010538","article-title":"The ins and outs of cholesterol in the vertebrate retina","volume":"51","author":"Fliesler","year":"2010","journal-title":"Journal of Lipid Research"},{"key":"bib24","doi-asserted-by":"publisher","first-page":"138","DOI":"10.1016\/0005-2760(82)90020-0","article-title":"Sterol composition of bovine retinal rod outer segment membranes and whole retinas","volume":"711","author":"Fliesler","year":"1982","journal-title":"Biochimica Et Biophysica Acta (BBA) - Lipids and Lipid Metabolism"},{"key":"bib25","doi-asserted-by":"publisher","first-page":"134","DOI":"10.1038\/ng.3448","article-title":"A large genome-wide association study of age-related macular degeneration highlights contributions of rare and common variants","volume":"48","author":"Fritsche","year":"2016","journal-title":"Nature Genetics"},{"key":"bib26","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2016.453","article-title":"Dysfunctional autophagy in RPE, a contributing factor in age-related macular degeneration","volume":"8","author":"Golestaneh","year":"2017","journal-title":"Cell Death & Disease"},{"key":"bib27","first-page":"1592","article-title":"The lipid composition of drusen, Bruch\u2019s Membrane, and Sclera by Hot Stage Polarizing Light Microscopy","volume":"42","author":"Haimovici","year":"2001","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"bib28","doi-asserted-by":"publisher","first-page":"1660","DOI":"10.1016\/j.ajpath.2014.02.007","article-title":"Age- and gene-dosage-dependent cre-induced abnormalities in the retinal pigment epithelium","volume":"184","author":"He","year":"2014","journal-title":"The American Journal of Pathology"},{"key":"bib29","doi-asserted-by":"publisher","first-page":"1378","DOI":"10.1167\/iovs.10-6347","article-title":"Generation of cre transgenic mice with postnatal RPE-Specific ocular expression","volume":"52","author":"Iacovelli","year":"2011","journal-title":"Investigative Opthalmology & Visual Science"},{"key":"bib30","doi-asserted-by":"publisher","first-page":"2084","DOI":"10.1194\/jlr.M087502","article-title":"ATP binding cassette family A protein 1 determines hexosylceramide and sphingomyelin levels in human and mouse plasma","volume":"59","author":"Iqbal","year":"2018","journal-title":"Journal of Lipid Research"},{"key":"bib31","doi-asserted-by":"publisher","first-page":"616","DOI":"10.1136\/bjo.2005.085076","article-title":"High density lipoprotein mediated lipid efflux from retinal pigment epithelial cells in culture","volume":"90","author":"Ishida","year":"2006","journal-title":"British Journal of Ophthalmology"},{"key":"bib32","doi-asserted-by":"publisher","first-page":"601","DOI":"10.1007\/978-1-4614-3209-8_76","article-title":"Analysis of mouse RPE sheet morphology gives discriminatory categories","volume":"801","author":"Jiang","year":"2014","journal-title":"Advances in Experimental Medicine and Biology"},{"key":"bib33","doi-asserted-by":"publisher","first-page":"2482","DOI":"10.1016\/j.ophtha.2015.08.002","article-title":"The incidence and progression of Age-Related macular degeneration over 15 years: the blue mountains eye study","volume":"122","author":"Joachim","year":"2015","journal-title":"Ophthalmology"},{"key":"bib34","doi-asserted-by":"publisher","first-page":"445","DOI":"10.1016\/j.nbd.2013.01.018","article-title":"ABCA1 influences neuroinflammation and neuronal death","volume":"54","author":"Karasinska","year":"2013","journal-title":"Neurobiology of Disease"},{"key":"bib35","doi-asserted-by":"publisher","first-page":"60","DOI":"10.1016\/j.exer.2015.12.008","article-title":"Hypoxic preconditioning protects photoreceptors against light damage independently of hypoxia inducible transcription factors in rods","volume":"146","author":"Kast","year":"2016","journal-title":"Experimental Eye Research"},{"key":"bib36","doi-asserted-by":"publisher","first-page":"1765","DOI":"10.1007\/s00018-016-2147-8","article-title":"Inflammation and its role in age-related macular degeneration","volume":"73","author":"Kauppinen","year":"2016","journal-title":"Cellular and Molecular Life Sciences"},{"key":"bib37","doi-asserted-by":"publisher","first-page":"197","DOI":"10.1146\/annurev-vision-111815-114407","article-title":"Retinoids and retinal diseases","volume":"2","author":"Kiser","year":"2016","journal-title":"Annual Review of Vision Science"},{"key":"bib38","doi-asserted-by":"publisher","first-page":"1210","DOI":"10.1016\/j.ophtha.2012.11.041","article-title":"Incidence of visual impairment over a 20-year period: the beaver dam eye study","volume":"120","author":"Klein","year":"2013","journal-title":"Ophthalmology"},{"key":"bib39","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1016\/j.nbd.2014.05.007","article-title":"ATP-binding cassette transporter A1: from metabolism to neurodegeneration","volume":"72","author":"Koldamova","year":"2014","journal-title":"Neurobiology of Disease"},{"key":"bib40","doi-asserted-by":"publisher","first-page":"659","DOI":"10.2337\/db11-1341","article-title":"Loss of both ABCA1 and ABCG1 results in increased disturbances in islet sterol homeostasis, inflammation, and impaired \u03b2-cell function","volume":"61","author":"Kruit","year":"2012","journal-title":"Diabetes"},{"key":"bib41","doi-asserted-by":"publisher","first-page":"11026","DOI":"10.1073\/pnas.0702504104","article-title":"The lipofuscin fluorophore A2E perturbs cholesterol metabolism in retinal pigment epithelial cells","volume":"104","author":"Lakkaraju","year":"2007","journal-title":"PNAS"},{"key":"bib42","doi-asserted-by":"publisher","first-page":"317","DOI":"10.1089\/jop.2016.0022","article-title":"Induced pluripotent stem Cell-Derived retinal pigmented epithelium: a comparative study between cell lines and differentiation methods","volume":"32","author":"Leach","year":"2016","journal-title":"Journal of Ocular Pharmacology and Therapeutics"},{"key":"bib43","doi-asserted-by":"publisher","first-page":"505","DOI":"10.1002\/iub.1165","article-title":"ATP-binding cassette transporters and cholesterol translocation","volume":"65","author":"Li","year":"2013","journal-title":"IUBMB Life"},{"key":"bib44","doi-asserted-by":"publisher","first-page":"1728","DOI":"10.1016\/S0140-6736(12)60282-7","article-title":"Age-related macular degeneration","volume":"379","author":"Lim","year":"2012","journal-title":"The Lancet"},{"key":"bib45","doi-asserted-by":"publisher","first-page":"1927","DOI":"10.1194\/jlr.M087361","article-title":"Directional ABCA1-mediated cholesterol efflux and apoB-lipoprotein secretion in the retinal pigment epithelium","volume":"59","author":"Lyssenko","year":"2018","journal-title":"Journal of Lipid Research"},{"key":"bib46","doi-asserted-by":"publisher","first-page":"1147","DOI":"10.1167\/iovs.04-1207","article-title":"Detailed characterization of the lipid composition of Detergent-Resistant membranes from photoreceptor rod outer segment membranes","volume":"46","author":"Martin","year":"2005","journal-title":"Investigative Opthalmology & Visual Science"},{"key":"bib47","doi-asserted-by":"publisher","first-page":"26933","DOI":"10.1074\/jbc.M110.130294","article-title":"SOX9, through interaction with microphthalmia-associated transcription factor (MITF) and OTX2, regulates BEST1 expression in the retinal pigment epithelium","volume":"285","author":"Masuda","year":"2010","journal-title":"Journal of Biological Chemistry"},{"key":"bib48","doi-asserted-by":"publisher","first-page":"E2630","DOI":"10.1073\/pnas.1418840112","article-title":"Bestrophin 1 is indispensable for volume regulation in human retinal pigment epithelium cells","volume":"112","author":"Milenkovic","year":"2015","journal-title":"PNAS"},{"key":"bib49","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1006\/exer.1996.0007","article-title":"Normally occurring loss of single cells and repair of resulting defects in retinal pigment epithelium in situ","volume":"62","author":"Nagai","year":"1996","journal-title":"Experimental Eye Research"},{"key":"bib50","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1016\/j.chroma.2016.02.054","article-title":"Comprehensive untargeted lipidomic analysis using core-shell C30 particle column and high field orbitrap mass spectrometer","volume":"1440","author":"Narv\u00e1ez-Rivas","year":"2016","journal-title":"Journal of Chromatography A"},{"key":"bib51","doi-asserted-by":"publisher","first-page":"4714","DOI":"10.1167\/iovs.14-14332","article-title":"Lack of paraoxonase 1 alters phospholipid composition, but not morphology and function of the mouse retina","volume":"55","author":"Oczos","year":"2014","journal-title":"Investigative Opthalmology & Visual Science"},{"key":"bib52","doi-asserted-by":"publisher","first-page":"448","DOI":"10.1016\/j.bbalip.2008.08.001","article-title":"Lipid droplets as dynamic organelles connecting storage and efflux of lipids","volume":"1791","author":"Olofsson","year":"2009","journal-title":"Biochimica Et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids"},{"key":"bib53","doi-asserted-by":"publisher","first-page":"1196","DOI":"10.1016\/S0161-6420(01)00580-2","article-title":"Delays in rod-mediated dark adaptation in early age-related maculopathy","volume":"108","author":"Owsley","year":"2001","journal-title":"Ophthalmology"},{"key":"bib54","doi-asserted-by":"publisher","first-page":"1728","DOI":"10.1016\/j.ophtha.2006.12.023","article-title":"Cone- and rod-mediated dark adaptation impairment in age-related maculopathy","volume":"114","author":"Owsley","year":"2007","journal-title":"Ophthalmology"},{"key":"bib55","doi-asserted-by":"publisher","first-page":"5231","DOI":"10.1016\/j.bmcl.2006.06.081","article-title":"Synthesis and evaluation of anilinohexafluoroisopropanols as activators\/modulators of LXRalpha and beta","volume":"16","author":"Panday","year":"2006","journal-title":"Bioorganic & Medicinal Chemistry Letters"},{"key":"bib56","doi-asserted-by":"publisher","first-page":"171","DOI":"10.1016\/S0161-6420(90)32619-2","article-title":"Aging changes in Bruch's Membrane","volume":"97","author":"Pauleikhoff","year":"1990","journal-title":"Ophthalmology"},{"key":"bib57","doi-asserted-by":"publisher","first-page":"7937","DOI":"10.1007\/s00216-014-8255-0","article-title":"LC\/MS lipid profiling from human serum: a new method for global lipid extraction","volume":"406","author":"Pellegrino","year":"2014","journal-title":"Analytical and Bioanalytical Chemistry"},{"key":"bib58","doi-asserted-by":"publisher","first-page":"1005","DOI":"10.1016\/j.ajo.2011.05.016","article-title":"Evaluation of new and established age-related macular degeneration susceptibility genes in the women's Health Initiative Sight Exam (WHI-SE) Study","volume":"152","author":"Peter","year":"2011","journal-title":"American Journal of Ophthalmology"},{"key":"bib59","doi-asserted-by":"publisher","first-page":"64","DOI":"10.1016\/j.preteyeres.2014.03.002","article-title":"Cholesterol in the retina: the best is yet to come","volume":"41","author":"Pikuleva","year":"2014","journal-title":"Progress in Retinal and Eye Research"},{"key":"bib60","doi-asserted-by":"publisher","first-page":"265","DOI":"10.1042\/bse0500265","article-title":"Lipid transport by mammalian ABC proteins","volume":"50","author":"Quazi","year":"2011","journal-title":"Essays in Biochemistry"},{"key":"bib61","doi-asserted-by":"publisher","first-page":"860","DOI":"10.1038\/nrn2007","article-title":"Macular degeneration: recent advances and therapeutic opportunities","volume":"7","author":"Rattner","year":"2006","journal-title":"Nature Reviews Neuroscience"},{"key":"bib62","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2350-10-1","article-title":"Multiple splice defects in ABCA1 cause low HDL-C in a family with hypoalphalipoproteinemia and premature coronary disease","volume":"10","author":"Rhyne","year":"2009","journal-title":"BMC Medical Genetics"},{"key":"bib63","doi-asserted-by":"publisher","first-page":"210","DOI":"10.1167\/iovs.13-13248","article-title":"Allergy is a protective factor against Age-Related macular degeneration","volume":"55","author":"Ristau","year":"2014","journal-title":"Investigative Opthalmology & Visual Science"},{"key":"bib64","doi-asserted-by":"publisher","first-page":"219","DOI":"10.1016\/j.exer.2018.07.014","article-title":"Conditional loss of Kcnj13 in the retinal pigment epithelium causes photoreceptor degeneration","volume":"176","author":"Roman","year":"2018","journal-title":"Experimental Eye Research"},{"key":"bib65","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1016\/j.exer.2008.07.010","article-title":"Sub-retinal drusenoid deposits in human retina: organization and composition","volume":"87","author":"Rudolf","year":"2008","journal-title":"Experimental Eye Research"},{"key":"bib66","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1146\/annurev-nutr-071811-150748","article-title":"Vitamin A metabolism in rod and cone visual cycles","volume":"32","author":"Saari","year":"2012","journal-title":"Annual Review of Nutrition"},{"key":"bib67","doi-asserted-by":"publisher","first-page":"2411","DOI":"10.1096\/fj.06-5895fje","article-title":"Differential role of Jak-STAT signaling in retinal degenerations","volume":"20","author":"Samardzija","year":"2006","journal-title":"The FASEB Journal"},{"key":"bib68","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1093\/hmg\/ddm304","article-title":"R91W mutation in Rpe65 leads to milder early-onset retinal dystrophy due to the generation of low levels of 11-cis-retinal","volume":"17","author":"Samardzija","year":"2008","journal-title":"Human Molecular Genetics"},{"key":"bib69","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1111\/j.1442-9071.1980.tb01670.x","article-title":"Drusen and their relationship to senile macular degeneration","volume":"8","author":"Sarks","year":"1980","journal-title":"Australian and New Zealand Journal of Ophthalmology"},{"key":"bib70","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1016\/j.pcad.2016.08.006","article-title":"Diagnosis and treatment of high density lipoprotein deficiency","volume":"59","author":"Schaefer","year":"2016","journal-title":"Progress in Cardiovascular Diseases"},{"key":"bib71","doi-asserted-by":"publisher","first-page":"2831","DOI":"10.1101\/gad.850400","article-title":"Role of LXRs in control of lipogenesis","volume":"14","author":"Schultz","year":"2000","journal-title":"Genes & Development"},{"key":"bib72","doi-asserted-by":"publisher","first-page":"549","DOI":"10.1016\/j.cmet.2013.03.009","article-title":"Impaired cholesterol efflux in senescent macrophages promotes age-related macular degeneration","volume":"17","author":"Sene","year":"2013","journal-title":"Cell Metabolism"},{"key":"bib73","doi-asserted-by":"publisher","first-page":"782","DOI":"10.1016\/j.survophthal.2018.05.005","article-title":"Subretinal drusenoid deposits AKA pseudodrusen","volume":"63","author":"Spaide","year":"2018","journal-title":"Survey of Ophthalmology"},{"key":"bib74","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1016\/j.exer.2017.09.008","article-title":"Regulated efflux of photoreceptor outer segment-derived cholesterol by human RPE cells","volume":"165","author":"Storti","year":"2017","journal-title":"Experimental Eye Research"},{"key":"bib75","doi-asserted-by":"publisher","first-page":"845","DOI":"10.1152\/physrev.00021.2004","article-title":"The retinal pigment epithelium in visual function","volume":"85","author":"Strauss","year":"2005","journal-title":"Physiological Reviews"},{"key":"bib76","doi-asserted-by":"publisher","first-page":"3366","DOI":"10.1074\/jbc.M116.770024","article-title":"MicroRNA-processing enzymes are essential for survival and function of mature retinal pigmented epithelial cells in mice","volume":"292","author":"Sundermeier","year":"2017","journal-title":"Journal of Biological Chemistry"},{"key":"bib77","doi-asserted-by":"publisher","first-page":"905","DOI":"10.1172\/JCI0216452","article-title":"Consequences of cellular cholesterol accumulation: basic concepts and physiological implications","volume":"110","author":"Tabas","year":"2002","journal-title":"Journal of Clinical Investigation"},{"key":"bib78","doi-asserted-by":"publisher","first-page":"1917","DOI":"10.1016\/j.ajpath.2012.01.017","article-title":"Evidence for baseline retinal pigment epithelium pathology in the Trp1-Cre mouse","volume":"180","author":"Thanos","year":"2012","journal-title":"The American Journal of Pathology"},{"key":"bib79","doi-asserted-by":"publisher","first-page":"1333","DOI":"10.1172\/JCI200523915","article-title":"Targeted inactivation of hepatic Abca1 causes profound hypoalphalipoproteinemia and kidney hypercatabolism of apoA-I","volume":"115","author":"Timmins","year":"2005","journal-title":"Journal of Clinical Investigation"},{"key":"bib80","first-page":"1319","article-title":"Intraretinal lipid transport is dependent on high density lipoprotein-like particles and class B scavenger receptors","volume":"12","author":"Tserentsoodol","year":"2006","journal-title":"Molecular Vision"},{"key":"bib81","doi-asserted-by":"publisher","first-page":"56","DOI":"10.1016\/j.preteyeres.2018.04.006","article-title":"A new perspective on lipid research in age-related macular degeneration","volume":"67","author":"van Leeuwen","year":"2018","journal-title":"Progress in Retinal and Eye Research"},{"key":"bib82","doi-asserted-by":"publisher","first-page":"156","DOI":"10.1007\/BF02633976","article-title":"An efficient method for routine Epstein-Barr virus immortalization of human B lymphocytes","volume":"31","author":"Wall","year":"1995","journal-title":"In Vitro Cellular & Developmental Biology - Animal"},{"key":"bib83","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0010329","article-title":"Abundant lipid and protein components of drusen","volume":"5","author":"Wang","year":"2010","journal-title":"PLOS ONE"},{"key":"bib84","doi-asserted-by":"publisher","first-page":"450","DOI":"10.1016\/j.exer.2015.09.015","article-title":"An easy, rapid method to isolate RPE cell protein from the mouse eye","volume":"145","author":"Wei","year":"2016","journal-title":"Experimental Eye Research"},{"key":"bib85","doi-asserted-by":"publisher","first-page":"29874","DOI":"10.1074\/jbc.M503603200","article-title":"The retinal G protein-coupled receptor (RGR) enhances isomerohydrolase activity independent of light","volume":"280","author":"Wenzel","year":"2005","journal-title":"Journal of Biological Chemistry"},{"key":"bib86","doi-asserted-by":"publisher","first-page":"195","DOI":"10.1016\/j.stem.2012.04.024","article-title":"Regulation of hematopoietic stem and progenitor cell mobilization by cholesterol efflux pathways","volume":"11","author":"Westerterp","year":"2012","journal-title":"Cell Stem Cell"},{"key":"bib87","doi-asserted-by":"publisher","first-page":"1456","DOI":"10.1161\/CIRCRESAHA.113.301086","article-title":"Deficiency of ATP-binding cassette transporters A1 and G1 in macrophages increases inflammation and accelerates atherosclerosis in mice","volume":"112","author":"Westerterp","year":"2013","journal-title":"Circulation Research"},{"key":"bib88","doi-asserted-by":"publisher","first-page":"1328","DOI":"10.1161\/ATVBAHA.115.306670","article-title":"Deficiency of ATP-Binding cassette transporters A1 and G1 in endothelial cells accelerates atherosclerosis in mice","volume":"36","author":"Westerterp","year":"2016","journal-title":"Arteriosclerosis, Thrombosis, and Vascular Biology"},{"key":"bib89","doi-asserted-by":"publisher","first-page":"407","DOI":"10.1136\/jcp.49.5.407","article-title":"The pathology of cornea in Tangier disease (familial high density lipoprotein deficiency)","volume":"49","author":"Winder","year":"1996","journal-title":"Journal of Clinical Pathology"},{"key":"bib90","doi-asserted-by":"publisher","first-page":"e106","DOI":"10.1016\/S2214-109X(13)70145-1","article-title":"Global prevalence of age-related macular degeneration and disease burden projection for 2020 and 2040: a systematic review and meta-analysis","volume":"2","author":"Wong","year":"2014","journal-title":"The Lancet Global Health"},{"key":"bib91","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0191279","article-title":"Cadherins in the retinal pigment epithelium (RPE) revisited: p-cadherin is the highly dominant cadherin expressed in human and mouse RPE in vivo","volume":"13","author":"Yang","year":"2018","journal-title":"PLOS ONE"},{"key":"bib92","doi-asserted-by":"publisher","first-page":"939","DOI":"10.1080\/15548627.2015.1041699","article-title":"Deletion of autophagy inducer RB1CC1 results in degeneration of the retinal pigment epithelium","volume":"11","author":"Yao","year":"2015","journal-title":"Autophagy"},{"key":"bib93","doi-asserted-by":"publisher","first-page":"4663","DOI":"10.1167\/iovs.10-7070","article-title":"Association of Variants in the LIPC and ABCA1 Genes with Intermediate and Large Drusen and Advanced Age-Related Macular Degeneration","volume":"52","author":"Yu","year":"2011","journal-title":"Investigative Opthalmology & Visual Science"},{"key":"bib94","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0037926","article-title":"Spatial distribution of the pathways of cholesterol homeostasis in human retina","volume":"7","author":"Zheng","year":"2012","journal-title":"PLOS ONE"},{"key":"bib95","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1194\/jlr.M053439","article-title":"Pathways of cholesterol homeostasis in mouse retina responsive to dietary and pharmacologic treatments","volume":"56","author":"Zheng","year":"2015","journal-title":"Journal of Lipid Research"},{"key":"bib96","doi-asserted-by":"publisher","first-page":"1775","DOI":"10.1016\/j.ophtha.2010.01.027","article-title":"Prevalence and significance of subretinal drusenoid deposits (reticular pseudodrusen) in age-related macular degeneration","volume":"117","author":"Zweifel","year":"2010","journal-title":"Ophthalmology"}],"container-title":["eLife"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/cdn.elifesciences.org\/articles\/45100\/elife-45100-v2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/cdn.elifesciences.org\/articles\/45100\/elife-45100-v2.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/elifesciences.org\/articles\/45100","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T20:56:36Z","timestamp":1697057796000},"score":1,"resource":{"primary":{"URL":"https:\/\/elifesciences.org\/articles\/45100"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,3,13]]},"references-count":96,"alternative-id":["10.7554\/eLife.45100"],"URL":"https:\/\/doi.org\/10.7554\/elife.45100","archive":["CLOCKSS"],"relation":{"has-review":[{"id-type":"doi","id":"10.7554\/eLife.45100.025","asserted-by":"object"},{"id-type":"doi","id":"10.7554\/eLife.45100.026","asserted-by":"object"}]},"ISSN":["2050-084X"],"issn-type":[{"value":"2050-084X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,3,13]]},"article-number":"e45100"}}