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Susceptibility to brief mating-induced death is caused by the loss of protection upon self-sperm depletion. Self-sperm maintains the expression of a DAF-2 insulin-like antagonist, INS-37, which promotes the nuclear localization of intestinal HLH-30\/TFEB, a key pro-longevity regulator. Mating induces the agonist INS-8, promoting HLH-30 nuclear exit and subsequent death. In opposition to the protective role of HLH-30 and DAF-16\/FOXO, TOR\/LET-363 and the IIS-regulated Zn-finger transcription factor PQM-1 promote seminal-fluid-induced killing. Self-sperm maintenance of nuclear HLH-30\/TFEB allows hermaphrodites to resist mating-induced death until self-sperm are exhausted, increasing the chances that mothers will survive through reproduction. Mothers combat males\u2019 hijacking of their IIS pathway by expressing an insulin antagonist that keeps her healthy through the activity of pro-longevity factors, as long as she has her own sperm to utilize.<\/jats:p>","DOI":"10.7554\/elife.46413","type":"journal-article","created":{"date-parts":[[2019,7,8]],"date-time":"2019-07-08T10:00:19Z","timestamp":1562580019000},"source":"Crossref","is-referenced-by-count":27,"title":["Insulin-like peptides and the mTOR-TFEB pathway protect Caenorhabditis elegans hermaphrodites from mating-induced death"],"prefix":"10.7554","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0365-8273","authenticated-orcid":true,"given":"Cheng","family":"Shi","sequence":"first","affiliation":[{"name":"Department of Molecular Biology, Princeton University, Princeton, United States"},{"name":"LSI Genomics, Princeton University, Princeton, United 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