{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T00:17:13Z","timestamp":1793060233357,"version":"4.5.0"},"reference-count":89,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2019,12,24]],"date-time":"2019-12-24T00:00:00Z","timestamp":1577145600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,12,24]],"date-time":"2019-12-24T00:00:00Z","timestamp":1577145600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,12,24]],"date-time":"2019-12-24T00:00:00Z","timestamp":1577145600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100004440","name":"Wellcome","doi-asserted-by":"publisher","award":["108070\/Z\/15\/Z"],"award-info":[{"award-number":["108070\/Z\/15\/Z"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000265","name":"Medical Research Council","doi-asserted-by":"publisher","award":["MR\/L018373\/1"],"award-info":[{"award-number":["MR\/L018373\/1"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000265","name":"Medical Research Council","doi-asserted-by":"publisher","award":["MR\/P001602\/1"],"award-info":[{"award-number":["MR\/P001602\/1"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004440","name":"Wellcome","doi-asserted-by":"publisher","award":["WT090323MA"],"award-info":[{"award-number":["WT090323MA"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000265","name":"Medical Research Council","doi-asserted-by":"publisher","award":["MC_UU_12014\/3"],"award-info":[{"award-number":["MC_UU_12014\/3"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["U24 HG006673"],"award-info":[{"award-number":["U24 HG006673"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000265","name":"Medical Research Council","doi-asserted-by":"publisher","award":["MR\/S00971X\/1"],"award-info":[{"award-number":["MR\/S00971X\/1"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Human cytomegalovirus (HCMV) extensively modulates host cells, downregulating &gt;900 human proteins during viral replication and degrading \u2265133 proteins shortly after infection. The mechanism of degradation of most host proteins remains unresolved, and the functions of many viral proteins are incompletely characterised. We performed a mass spectrometry-based interactome analysis of 169 tagged, stably-expressed canonical strain Merlin HCMV proteins, and two non-canonical HCMV proteins, in infected cells. This identified a network of &gt;3400 virus-host and &gt;150 virus-virus protein interactions, providing insights into functions for multiple viral genes. Domain analysis predicted binding of the viral UL25 protein to SH3 domains of NCK Adaptor Protein-1. Viral interacting proteins were identified for 31\/133 degraded host targets. Finally, the uncharacterised, non-canonical ORFL147C protein was found to interact with elements of the mRNA splicing machinery, and a mutational study suggested its importance in viral replication. The interactome data will be important for future studies of herpesvirus infection.<\/jats:p>","DOI":"10.7554\/elife.49894","type":"journal-article","created":{"date-parts":[[2019,12,24]],"date-time":"2019-12-24T08:00:19Z","timestamp":1577174419000},"source":"Crossref","is-referenced-by-count":110,"title":["Human cytomegalovirus interactome analysis identifies degradation hubs, domain associations and viral protein functions"],"prefix":"10.7554","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0467-8989","authenticated-orcid":true,"given":"Luis V","family":"Nobre","sequence":"first","affiliation":[{"name":"Cambridge Institute for Medical Research, University of Cambridge, Cambridge, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9958-4699","authenticated-orcid":true,"given":"Katie","family":"Nightingale","sequence":"additional","affiliation":[{"name":"Cambridge Institute for Medical Research, University of Cambridge, Cambridge, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin J","family":"Ravenhill","sequence":"additional","affiliation":[{"name":"Cambridge Institute for Medical Research, University of Cambridge, Cambridge, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robin","family":"Antrobus","sequence":"additional","affiliation":[{"name":"Cambridge Institute for Medical Research, University of Cambridge, Cambridge, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6927-2985","authenticated-orcid":true,"given":"Lior","family":"Soday","sequence":"additional","affiliation":[{"name":"Cambridge Institute for Medical Research, University of Cambridge, Cambridge, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jenna","family":"Nichols","sequence":"additional","affiliation":[{"name":"MRC-University of Glasgow Centre for Virus Research, Glasgow, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3569-4500","authenticated-orcid":true,"given":"James A","family":"Davies","sequence":"additional","affiliation":[{"name":"Division of Infection and Immunity, Cardiff University School of Medicine, Cardiff, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sepehr","family":"Seirafian","sequence":"additional","affiliation":[{"name":"Division of Infection and Immunity, Cardiff University School of Medicine, Cardiff, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2243-4964","authenticated-orcid":true,"given":"Eddie CY","family":"Wang","sequence":"additional","affiliation":[{"name":"Division of Infection and Immunity, Cardiff University School of Medicine, Cardiff, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4991-9128","authenticated-orcid":true,"given":"Andrew J","family":"Davison","sequence":"additional","affiliation":[{"name":"MRC-University of Glasgow Centre for Virus Research, Glasgow, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5623-0126","authenticated-orcid":true,"given":"Gavin WG","family":"Wilkinson","sequence":"additional","affiliation":[{"name":"Division of Infection and Immunity, Cardiff University School of Medicine, Cardiff, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6799-1182","authenticated-orcid":true,"given":"Richard J","family":"Stanton","sequence":"additional","affiliation":[{"name":"Division of Infection and Immunity, Cardiff University School of Medicine, Cardiff, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1822-1173","authenticated-orcid":true,"given":"Edward L","family":"Huttlin","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Harvard Medical School, Boston, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3196-5545","authenticated-orcid":true,"given":"Michael P","family":"Weekes","sequence":"additional","affiliation":[{"name":"Cambridge Institute for Medical Research, University of Cambridge, Cambridge, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4374","published-online":{"date-parts":[[2019,12,24]]},"reference":[{"key":"bib1","doi-asserted-by":"publisher","first-page":"585","DOI":"10.1038\/nature11354","article-title":"Interaction landscape of membrane-protein complexes in Saccharomyces cerevisiae","volume":"489","author":"Babu","year":"2012","journal-title":"Nature"},{"key":"bib2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-16262-z","article-title":"Long-Read sequencing of human Cytomegalovirus transcriptome reveals RNA isoforms carrying distinct coding potentials","volume":"7","author":"Bal\u00e1zs","year":"2017","journal-title":"Scientific Reports"},{"key":"bib3","doi-asserted-by":"publisher","first-page":"68","DOI":"10.1038\/nature09204","article-title":"Network organization of the human autophagy system","volume":"466","author":"Behrends","year":"2010","journal-title":"Nature"},{"key":"bib4","doi-asserted-by":"publisher","first-page":"289","DOI":"10.1111\/j.2517-6161.1995.tb02031.x","article-title":"Controlling the false discovery rate: a practical and powerful approach to multiple testing","volume":"57","author":"Benjamini","year":"1995","journal-title":"Journal of the Royal Statistical Society: Series B"},{"key":"bib5","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms3863","article-title":"Proteasomal degradation of Nck1 but not Nck2 regulates RhoA activation and actin dynamics","volume":"4","author":"Buvall","year":"2013","journal-title":"Nature Communications"},{"key":"bib6","doi-asserted-by":"publisher","first-page":"D588","DOI":"10.1093\/nar\/gku830","article-title":"VirusMentha: a new resource for virus-host protein interactions","volume":"43","author":"Calderone","year":"2015","journal-title":"Nucleic Acids Research"},{"key":"bib7","doi-asserted-by":"publisher","first-page":"264","DOI":"10.1016\/j.virol.2004.05.010","article-title":"Evidence that the human Cytomegalovirus 46-kDa UL72 protein is not an active dUTPase but a late protein dispensable for replication in fibroblasts","volume":"325","author":"Caposio","year":"2004","journal-title":"Virology"},{"key":"bib8","doi-asserted-by":"publisher","first-page":"16546","DOI":"10.1074\/jbc.M109.085308","article-title":"Small molecular compounds inhibit HIV-1 replication through specifically stabilizing APOBEC3G","volume":"285","author":"Cen","year":"2010","journal-title":"Journal of Biological Chemistry"},{"key":"bib9","doi-asserted-by":"publisher","first-page":"D816","DOI":"10.1093\/nar\/gks1158","article-title":"The BioGRID interaction database: 2013 update","volume":"41","author":"Chatr-Aryamontri","year":"2013","journal-title":"Nucleic Acids Research"},{"key":"bib10","doi-asserted-by":"publisher","first-page":"523","DOI":"10.1016\/S0092-8674(02)00735-3","article-title":"Molecular architecture and functional model of the endocytic AP2 complex","volume":"109","author":"Collins","year":"2002","journal-title":"Cell"},{"key":"bib11","doi-asserted-by":"publisher","first-page":"349","DOI":"10.1016\/j.molcel.2014.11.026","article-title":"Global mapping of herpesvirus-host protein complexes reveals a transcription strategy for late genes","volume":"57","author":"Davis","year":"2015","journal-title":"Molecular Cell"},{"key":"bib12","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00907-17","article-title":"A Tyrosine-Based trafficking motif of the tegument protein pUL71 is crucial for human Cytomegalovirus secondary envelopment","volume":"92","author":"Dietz","year":"2018","journal-title":"Journal of Virology"},{"key":"bib13","doi-asserted-by":"publisher","first-page":"1301","DOI":"10.1099\/vir.0.79888-0","article-title":"Genetic content of wild-type human Cytomegalovirus","volume":"85","author":"Dolan","year":"2004","journal-title":"Journal of General Virology"},{"key":"bib14","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1038\/nmeth1019","article-title":"Target-decoy search strategy for increased confidence in large-scale protein identifications by mass spectrometry","volume":"4","author":"Elias","year":"2007","journal-title":"Nature Methods"},{"key":"bib15","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1007\/978-1-60761-444-9_5","article-title":"Target-decoy search strategy for mass spectrometry-based proteomics","volume":"604","author":"Elias","year":"2010","journal-title":"Methods in Molecular Biology"},{"key":"bib16","doi-asserted-by":"publisher","first-page":"976","DOI":"10.1016\/1044-0305(94)80016-2","article-title":"An approach to correlate tandem mass spectral data of peptides with amino acid sequences in a protein database","volume":"5","author":"Eng","year":"1994","journal-title":"Journal of the American Society for Mass Spectrometry"},{"key":"bib17","doi-asserted-by":"publisher","first-page":"D649","DOI":"10.1093\/nar\/gkx1132","article-title":"The reactome pathway knowledgebase","volume":"46","author":"Fabregat","year":"2018","journal-title":"Nucleic Acids Research"},{"key":"bib18","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004058","article-title":"Two novel human Cytomegalovirus NK cell evasion functions target MICA for lysosomal degradation","volume":"10","author":"Fielding","year":"2014","journal-title":"PLOS Pathogens"},{"key":"bib19","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.22206","article-title":"Control of immune ligands by members of a Cytomegalovirus gene expansion suppresses natural killer cell activation","volume":"6","author":"Fielding","year":"2017","journal-title":"eLife"},{"key":"bib20","doi-asserted-by":"publisher","first-page":"D222","DOI":"10.1093\/nar\/gkt1223","article-title":"Pfam: the protein families database","volume":"42","author":"Finn","year":"2014","journal-title":"Nucleic Acids Research"},{"key":"bib21","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0075217","article-title":"Molecular phylogeny of a RING E3 ubiquitin ligase, conserved in eukaryotic cells and dominated by homologous components, the muskelin\/RanBPM\/CTLH complex","volume":"8","author":"Francis","year":"2013","journal-title":"PLOS\u00a0ONE"},{"key":"bib22","doi-asserted-by":"publisher","DOI":"10.1038\/srep27114","article-title":"A protein interaction map for cell-cell adhesion regulators identifies DUSP23 as a novel phosphatase for \u03b2-catenin","volume":"6","author":"Gallegos","year":"2016","journal-title":"Scientific Reports"},{"key":"bib23","doi-asserted-by":"publisher","first-page":"19755","DOI":"10.1073\/pnas.1115861108","article-title":"High-resolution human cytomegalovirus transcriptome","volume":"108","author":"Gatherer","year":"2011","journal-title":"PNAS"},{"key":"bib24","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01001-18","article-title":"A Single-Cell approach to the elusive latent human Cytomegalovirus transcriptome","volume":"9","author":"Goodrum","year":"2018","journal-title":"mBio"},{"key":"bib25","doi-asserted-by":"publisher","first-page":"1326","DOI":"10.1074\/mcp.M500339-MCP200","article-title":"Optimization and use of peptide mass measurement accuracy in shotgun proteomics","volume":"5","author":"Haas","year":"2006","journal-title":"Molecular & Cellular Proteomics"},{"key":"bib26","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2017.00005","article-title":"The multifaceted role of SNARE proteins in membrane fusion","volume":"8","author":"Han","year":"2017","journal-title":"Frontiers in Physiology"},{"key":"bib27","doi-asserted-by":"publisher","first-page":"1277","DOI":"10.1038\/ncb1490","article-title":"CUL4-DDB1 ubiquitin ligase interacts with multiple WD40-repeat proteins and regulates histone methylation","volume":"8","author":"Higa","year":"2006","journal-title":"Nature Cell Biology"},{"key":"bib28","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004811","article-title":"Plasma membrane profiling defines an expanded class of cell surface proteins selectively targeted for degradation by HCMV US2 in cooperation with UL141","volume":"11","author":"Hsu","year":"2015","journal-title":"PLOS Pathogens"},{"key":"bib29","doi-asserted-by":"publisher","first-page":"44","DOI":"10.1038\/nprot.2008.211","article-title":"Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources","volume":"4","author":"Huang","year":"2009","journal-title":"Nature Protocols"},{"key":"bib30","doi-asserted-by":"publisher","first-page":"1174","DOI":"10.1016\/j.cell.2010.12.001","article-title":"A tissue-specific atlas of mouse protein phosphorylation and expression","volume":"143","author":"Huttlin","year":"2010","journal-title":"Cell"},{"key":"bib31","doi-asserted-by":"publisher","first-page":"425","DOI":"10.1016\/j.cell.2015.06.043","article-title":"The BioPlex network: a systematic exploration of the human interactome","volume":"162","author":"Huttlin","year":"2015","journal-title":"Cell"},{"key":"bib32","doi-asserted-by":"publisher","first-page":"505","DOI":"10.1038\/nature22366","article-title":"Architecture of the human interactome defines protein communities and disease networks","volume":"545","author":"Huttlin","year":"2017","journal-title":"Nature"},{"key":"bib33","doi-asserted-by":"publisher","first-page":"6629","DOI":"10.1128\/JVI.00384-11","article-title":"The human Cytomegalovirus gene products essential for late viral gene expression assemble into prereplication complexes before viral DNA replication","volume":"85","author":"Isomura","year":"2011","journal-title":"Journal of Virology"},{"key":"bib34","doi-asserted-by":"publisher","first-page":"1444","DOI":"10.1111\/j.1600-0854.2011.01236.x","article-title":"The polymorphic HCMV glycoprotein UL20 is targeted for lysosomal degradation by multiple cytoplasmic dileucine motifs","volume":"12","author":"Jelcic","year":"2011","journal-title":"Traffic"},{"key":"bib35","doi-asserted-by":"publisher","first-page":"923","DOI":"10.1038\/nmeth1113","article-title":"Semi-supervised learning for peptide identification from shotgun proteomics datasets","volume":"4","author":"K\u00e4ll","year":"2007","journal-title":"Nature Methods"},{"key":"bib36","doi-asserted-by":"publisher","first-page":"12799","DOI":"10.1074\/jbc.M116.724245","article-title":"Nephrin suppresses hippo signaling through the adaptor proteins nck and WTIP","volume":"291","author":"Keyvani Chahi","year":"2016","journal-title":"Journal of Biological Chemistry"},{"key":"bib37","doi-asserted-by":"publisher","first-page":"325","DOI":"10.1016\/j.molcel.2011.08.025","article-title":"Systematic and quantitative assessment of the ubiquitin-modified proteome","volume":"44","author":"Kim","year":"2011","journal-title":"Molecular Cell"},{"key":"bib38","doi-asserted-by":"publisher","first-page":"196","DOI":"10.1099\/jgv.0.000336","article-title":"Degradation of host ubiquitin E3 ligase itch by human Cytomegalovirus UL42","volume":"97","author":"Koshizuka","year":"2016","journal-title":"Journal of General Virology"},{"key":"bib39","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms14321","article-title":"Targeting the latent Cytomegalovirus reservoir with an antiviral fusion toxin protein","volume":"8","author":"Krishna","year":"2017","journal-title":"Nature Communications"},{"key":"bib40","doi-asserted-by":"publisher","DOI":"10.3390\/v10080445","article-title":"US28: hcmv\u2019s Swiss Army Knife","volume":"10","author":"Krishna","year":"2018","journal-title":"Viruses"},{"key":"bib41","doi-asserted-by":"publisher","first-page":"567","DOI":"10.1006\/jmbi.2000.4315","article-title":"Predicting transmembrane protein topology with a hidden markov model: application to complete genomes","volume":"305","author":"Krogh","year":"2001","journal-title":"Journal of Molecular Biology"},{"key":"bib42","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1007\/s12551-012-0081-z","article-title":"SH3 domains: modules of protein-protein interactions","volume":"5","author":"Kurochkina","year":"2013","journal-title":"Biophysical Reviews"},{"key":"bib43","doi-asserted-by":"publisher","first-page":"794","DOI":"10.1038\/cmi.2017.76","article-title":"Interferons command Trim22 to fight against viruses","volume":"14","author":"Lian","year":"2017","journal-title":"Cellular & Molecular Immunology"},{"key":"bib44","doi-asserted-by":"publisher","first-page":"D857","DOI":"10.1093\/nar\/gkr930","article-title":"MINT, the molecular interaction database: 2012 update","volume":"40","author":"Licata","year":"2012","journal-title":"Nucleic Acids Research"},{"key":"bib45","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt972","article-title":"A fluorescence-based assay suitable for quantitative analysis of deadenylase enzyme activity","volume":"42","author":"Maryati","year":"2014","journal-title":"Nucleic Acids Research"},{"key":"bib46","doi-asserted-by":"publisher","first-page":"7150","DOI":"10.1021\/ac502040v","article-title":"MultiNotch MS3 enables accurate, sensitive, and multiplexed detection of differential expression across Cancer cell line proteomes","volume":"86","author":"McAlister","year":"2014","journal-title":"Analytical Chemistry"},{"key":"bib47","doi-asserted-by":"publisher","first-page":"325","DOI":"10.2174\/1389203013380964","article-title":"Evolution of the dUTPase gene of mammalian and avian herpesviruses","volume":"2","author":"McGeehan","year":"2001","journal-title":"Current Protein & Peptide Science"},{"key":"bib48","doi-asserted-by":"publisher","first-page":"855","DOI":"10.1099\/0022-1317-82-4-855","article-title":"Human telomerase reverse transcriptase-immortalized MRC-5 and HCA2 human fibroblasts are fully permissive for human Cytomegalovirus","volume":"82","author":"McSharry","year":"2001","journal-title":"Journal of General Virology"},{"key":"bib49","doi-asserted-by":"publisher","first-page":"502","DOI":"10.1152\/physiolgenomics.00031.2016","article-title":"Comprehensive database of human E3 ubiquitin ligases: application to aquaporin-2 regulation","volume":"48","author":"Medvar","year":"2016","journal-title":"Physiological Genomics"},{"key":"bib50","doi-asserted-by":"publisher","first-page":"3370","DOI":"10.1128\/JVI.06556-11","article-title":"A leucine zipper motif of a tegument protein triggers final envelopment of human Cytomegalovirus","volume":"86","author":"Meissner","year":"2012","journal-title":"Journal of Virology"},{"key":"bib51","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.40009","article-title":"The sterol-responsive RNF145 E3 ubiquitin ligase mediates the degradation of HMG-CoA reductase together with gp78 and Hrd1","volume":"7","author":"Menzies","year":"2018","journal-title":"eLife"},{"key":"bib52","volume-title":"Cytomegaloviruses","author":"Mocarski","year":"2013"},{"key":"bib53","volume-title":"Human Herpesviruses: Biology, Therapy, and Immunoprophylaxis","author":"Mocarski Jr","year":"2007"},{"key":"bib54","doi-asserted-by":"publisher","first-page":"1187","DOI":"10.1038\/nbt.1496","article-title":"Small-molecule inhibition of HIV-1 vif","volume":"26","author":"Nathans","year":"2008","journal-title":"Nature Biotechnology"},{"key":"bib55","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003224","article-title":"Structure of human Cytomegalovirus UL141 binding to TRAIL-R2 reveals novel, non-canonical death receptor interactions","volume":"9","author":"Nem\u010dovi\u010dov\u00e1","year":"2013","journal-title":"PLOS\u00a0Pathogens"},{"key":"bib56","doi-asserted-by":"publisher","DOI":"10.1186\/1478-811X-12-21","article-title":"Non-overlapping functions of Nck1 and Nck2 adaptor proteins in T cell activation","volume":"12","author":"Ngoenkam","year":"2014","journal-title":"Cell Communication and Signaling"},{"key":"bib57","doi-asserted-by":"publisher","first-page":"273","DOI":"10.1086\/338624","article-title":"High risk of death due to bacterial and fungal infection among Cytomegalovirus (CMV)-seronegative recipients of stem cell transplants from seropositive donors: evidence for indirect effects of primary CMV infection","volume":"185","author":"Nichols","year":"2002","journal-title":"The Journal of Infectious Diseases"},{"key":"bib58","doi-asserted-by":"publisher","first-page":"447","DOI":"10.1016\/j.chom.2018.07.011","article-title":"High-Definition analysis of host protein stability during human Cytomegalovirus infection reveals antiviral factors and viral evasion mechanisms","volume":"24","author":"Nightingale","year":"2018","journal-title":"Cell Host & Microbe"},{"key":"bib59","doi-asserted-by":"publisher","first-page":"8651","DOI":"10.1128\/JVI.01052-13","article-title":"Cytomegalovirus UL91 is essential for transcription of viral true late (\u03b32) genes","volume":"87","author":"Omoto","year":"2013","journal-title":"Journal of Virology"},{"key":"bib60","doi-asserted-by":"publisher","first-page":"120","DOI":"10.1128\/JVI.02983-13","article-title":"Transcription of true late (\u03b32) cytomegalovirus genes requires UL92 function that is conserved among beta- and gammaherpesviruses","volume":"88","author":"Omoto","year":"2014","journal-title":"Journal of Virology"},{"key":"bib61","doi-asserted-by":"publisher","first-page":"D358","DOI":"10.1093\/nar\/gkt1115","article-title":"The MIntAct project--IntAct as a common curation platform for 11 molecular interaction databases","volume":"42","author":"Orchard","year":"2014","journal-title":"Nucleic Acids Research"},{"key":"bib62","doi-asserted-by":"publisher","first-page":"2033","DOI":"10.1084\/jem.20091793","article-title":"The HCMV membrane glycoprotein US10 selectively targets HLA-G for degradation","volume":"207","author":"Park","year":"2010","journal-title":"The Journal of Experimental Medicine"},{"key":"bib63","doi-asserted-by":"publisher","first-page":"10504","DOI":"10.1074\/jbc.M114.626903","article-title":"Identification of a novel HIV-1 inhibitor targeting Vif-dependent degradation of human APOBEC3G protein","volume":"290","author":"Pery","year":"2015","journal-title":"Journal of Biological Chemistry"},{"key":"bib64","doi-asserted-by":"publisher","first-page":"5502","DOI":"10.1128\/JVI.67.9.5502-5513.1993","article-title":"Spliced transcripts of human Cytomegalovirus","volume":"67","author":"Rawlinson","year":"1993","journal-title":"Journal of Virology"},{"key":"bib65","doi-asserted-by":"publisher","first-page":"4140","DOI":"10.1073\/pnas.0408994102","article-title":"Latency, chromatin remodeling, and reactivation of human Cytomegalovirus in the dendritic cells of healthy carriers","volume":"102","author":"Reeves","year":"2005","journal-title":"PNAS"},{"key":"bib66","doi-asserted-by":"publisher","first-page":"D1040","DOI":"10.1093\/nar\/gks1215","article-title":"Interferome v2.0: an updated database of annotated interferon-regulated genes","volume":"41","author":"Rusinova","year":"2013","journal-title":"Nucleic Acids Research"},{"key":"bib67","doi-asserted-by":"publisher","DOI":"10.1098\/rsob.170081","article-title":"Cell signalling pathway regulation by RanBPM: molecular insights and disease implications","volume":"7","author":"Salemi","year":"2017","journal-title":"Open Biology"},{"key":"bib68","doi-asserted-by":"publisher","first-page":"2463","DOI":"10.1128\/JVI.01926-12","article-title":"Human Cytomegalovirus pUL29\/28 and pUL38 repression of p53-regulated p21CIP1 and caspase 1 promoters during infection","volume":"87","author":"Savaryn","year":"2013","journal-title":"Journal of Virology"},{"key":"bib69","doi-asserted-by":"publisher","first-page":"337","DOI":"10.1038\/nature10098","article-title":"Global quantification of mammalian gene expression control","volume":"473","author":"Schwanh\u00e4usser","year":"2011","journal-title":"Nature"},{"key":"bib70","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00047-19","article-title":"The transcriptome of latent human Cytomegalovirus","volume":"93","author":"Schwartz","year":"2019","journal-title":"Journal of Virology"},{"key":"bib71","article-title":"An Analysis of Human Cytomegalovirus Gene Usage","author":"Seirafian","year":"2012","unstructured":"Seirafian S. 2012. An Analysis of Human Cytomegalovirus Gene Usage. Cardiff University. http:\/\/orca.cf.ac.uk\/46644\/."},{"key":"bib72","doi-asserted-by":"publisher","first-page":"2498","DOI":"10.1101\/gr.1239303","article-title":"Cytoscape: a software environment for integrated models of biomolecular interaction networks","volume":"13","author":"Shannon","year":"2003","journal-title":"Genome Research"},{"key":"bib73","doi-asserted-by":"publisher","first-page":"324","DOI":"10.1016\/j.chom.2013.02.003","article-title":"Human Cytomegalovirus glycoprotein UL141 targets the TRAIL death receptors to thwart host innate antiviral defenses","volume":"13","author":"Smith","year":"2013","journal-title":"Cell Host & Microbe"},{"key":"bib74","doi-asserted-by":"publisher","first-page":"389","DOI":"10.1016\/j.cell.2009.04.042","article-title":"Defining the human deubiquitinating enzyme interaction landscape","volume":"138","author":"Sowa","year":"2009","journal-title":"Cell"},{"key":"bib75","doi-asserted-by":"publisher","first-page":"7860","DOI":"10.1128\/JVI.02247-06","article-title":"Cytomegalovirus destruction of focal adhesions revealed in a high-throughput western blot analysis of cellular protein expression","volume":"81","author":"Stanton","year":"2007","journal-title":"Journal of Virology"},{"key":"bib76","doi-asserted-by":"publisher","first-page":"3191","DOI":"10.1172\/JCI42955","article-title":"Reconstruction of the complete human Cytomegalovirus genome in a BAC reveals RL13 to be a potent inhibitor of replication","volume":"120","author":"Stanton","year":"2010","journal-title":"Journal of Clinical Investigation"},{"key":"bib77","doi-asserted-by":"publisher","first-page":"201","DOI":"10.1016\/j.chom.2014.07.005","article-title":"HCMV pUL135 remodels the actin cytoskeleton to impair immune recognition of infected cells","volume":"16","author":"Stanton","year":"2014","journal-title":"Cell Host & Microbe"},{"key":"bib78","doi-asserted-by":"publisher","first-page":"1088","DOI":"10.1126\/science.1227919","article-title":"Decoding human cytomegalovirus","volume":"338","author":"Stern-Ginossar","year":"2012","journal-title":"Science"},{"key":"bib79","doi-asserted-by":"publisher","first-page":"1759","DOI":"10.1099\/0022-1317-65-10-1759","article-title":"Enhanced replication of human Cytomegalovirus in human fibroblasts treated with dexamethasone","volume":"65 ( Pt 10","author":"Tanaka","year":"1984","journal-title":"Journal of General Virology"},{"key":"bib80","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000965","article-title":"Human Cytomegalovirus UL29\/28 protein interacts with components of the NuRD complex which promote accumulation of immediate-early RNA","volume":"6","author":"Terhune","year":"2010","journal-title":"PLOS Pathogens"},{"key":"bib81","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1007\/978-3-642-59421-2_3","article-title":"The HCMV gene products US2 and US11 target MHC class I molecules for degradation in the cytosol","volume":"269","author":"van der Wal","year":"2002","journal-title":"Current Topics in Microbiology and Immunology"},{"key":"bib82","doi-asserted-by":"publisher","first-page":"D447","DOI":"10.1093\/nar\/gkv1145","article-title":"2016 update of the PRIDE database and its related tools","volume":"44","author":"Vizca\u00edno","year":"2016","journal-title":"Nucleic Acids Research"},{"key":"bib83","doi-asserted-by":"publisher","first-page":"631","DOI":"10.1002\/cphy.c170023","article-title":"Thick filament protein network, functions, and disease association","volume":"8","author":"Wang","year":"2018","journal-title":"Comprehensive Physiology"},{"key":"bib84","doi-asserted-by":"publisher","first-page":"199","DOI":"10.1126\/science.1235047","article-title":"Latency-associated degradation of the MRP1 drug transporter during latent human Cytomegalovirus infection","volume":"340","author":"Weekes","year":"2013","journal-title":"Science"},{"key":"bib85","doi-asserted-by":"publisher","first-page":"1460","DOI":"10.1016\/j.cell.2014.04.028","article-title":"Quantitative temporal viromics: an approach to investigate host-pathogen interaction","volume":"157","author":"Weekes","year":"2014","journal-title":"Cell"},{"key":"bib86","doi-asserted-by":"publisher","first-page":"769","DOI":"10.1016\/S0092-8674(00)81054-5","article-title":"The human Cytomegalovirus US11 gene product dislocates MHC class I heavy chains from the endoplasmic reticulum to the cytosol","volume":"84","author":"Wiertz","year":"1996","journal-title":"Cell"},{"key":"bib87","doi-asserted-by":"publisher","first-page":"206","DOI":"10.1016\/j.jcv.2007.10.027","article-title":"Modulation of natural killer cells by human Cytomegalovirus","volume":"41","author":"Wilkinson","year":"2008","journal-title":"Journal of Clinical Virology"},{"key":"bib88","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M111.009654","article-title":"Correct interpretation of comprehensive phosphorylation dynamics requires normalization by protein expression changes","volume":"10","author":"Wu","year":"2011","journal-title":"Molecular & Cellular Proteomics"},{"key":"bib89","doi-asserted-by":"publisher","first-page":"1081","DOI":"10.1016\/j.molcel.2018.05.009","article-title":"PABP cooperates with the CCR4-NOT complex to promote mRNA deadenylation and block precocious decay","volume":"70","author":"Yi","year":"2018","journal-title":"Molecular Cell"}],"container-title":["eLife"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/cdn.elifesciences.org\/articles\/49894\/elife-49894-v2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/cdn.elifesciences.org\/articles\/49894\/elife-49894-v2.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/elifesciences.org\/articles\/49894","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T21:03:15Z","timestamp":1697058195000},"score":1,"resource":{"primary":{"URL":"https:\/\/elifesciences.org\/articles\/49894"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,12,24]]},"references-count":89,"alternative-id":["10.7554\/eLife.49894"],"URL":"https:\/\/doi.org\/10.7554\/elife.49894","archive":["CLOCKSS"],"relation":{"references":[{"id-type":"accession","id":"PXD009945","asserted-by":"subject"}],"is-supplemented-by":[{"id-type":"accession","id":"PXD014845","asserted-by":"subject"}],"has-review":[{"id-type":"doi","id":"10.7554\/eLife.49894.sa2","asserted-by":"object"},{"id-type":"doi","id":"10.7554\/eLife.49894.sa1","asserted-by":"object"}]},"ISSN":["2050-084X"],"issn-type":[{"value":"2050-084X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,12,24]]},"article-number":"e49894"}}