{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T20:59:18Z","timestamp":1790801958160,"version":"4.1.0"},"reference-count":73,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2019,10,2]],"date-time":"2019-10-02T00:00:00Z","timestamp":1569974400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,10,2]],"date-time":"2019-10-02T00:00:00Z","timestamp":1569974400000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,10,2]],"date-time":"2019-10-02T00:00:00Z","timestamp":1569974400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010438","name":"Francis Crick Institute","doi-asserted-by":"publisher","award":["FC001189"],"award-info":[{"award-number":["FC001189"]}],"id":[{"id":"10.13039\/100010438","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","award":["AI1218885"],"award-info":[{"award-number":["AI1218885"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","award":["AI139201"],"award-info":[{"award-number":["AI139201"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010438","name":"Francis Crick Institute","doi-asserted-by":"publisher","award":["FC001999"],"award-info":[{"award-number":["FC001999"]}],"id":[{"id":"10.13039\/100010438","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","award":["AI137767"],"award-info":[{"award-number":["AI137767"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Toxoplasma gondii contains a limited subset of actin binding proteins. Here we show that the putative actin regulator cyclase-associated protein (CAP) is present in two different isoforms and its deletion leads to significant defects in some but not all actin dependent processes. We observe defects in cell-cell communication, daughter cell orientation and the juxtanuclear accumulation of actin, but only modest defects in synchronicity of division and no defect in the replication of the apicoplast. 3D electron microscopy reveals that loss of CAP results in a defect in formation of a normal central residual body, but parasites remain connected within the vacuole. This dissociates synchronicity of division and parasite rosetting and reveals that establishment and maintenance of the residual body may be more complex than previously thought. These results highlight the different spatial requirements for F-actin regulation in Toxoplasma which appear to be achieved by partially overlapping functions of actin regulators.<\/jats:p>","DOI":"10.7554\/elife.50598","type":"journal-article","created":{"date-parts":[[2019,10,2]],"date-time":"2019-10-02T08:00:17Z","timestamp":1570003217000},"source":"Crossref","is-referenced-by-count":70,"title":["Differential requirements for cyclase-associated protein (CAP) in actin-dependent processes of Toxoplasma gondii"],"prefix":"10.7554","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7431-7156","authenticated-orcid":true,"given":"Alex","family":"Hunt","sequence":"first","affiliation":[{"name":"Signalling in Apicomplexan Parasites Laboratory, The Francis Crick Institute, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4608-7669","authenticated-orcid":true,"given":"Matthew Robert Geoffrey","family":"Russell","sequence":"additional","affiliation":[{"name":"Electron Microscopy Science Technology Platform, The Francis Crick Institute, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7227-4348","authenticated-orcid":true,"given":"Jeanette","family":"Wagener","sequence":"additional","affiliation":[{"name":"Signalling in Apicomplexan Parasites Laboratory, The Francis Crick Institute, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2584-4694","authenticated-orcid":true,"given":"Robyn","family":"Kent","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, University of Vermont Larner College of Medicine, Burlington, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Romain","family":"Carmeille","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, University of Connecticut, Storrs, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher J","family":"Peddie","sequence":"additional","affiliation":[{"name":"Electron Microscopy Science Technology Platform, The Francis Crick Institute, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucy","family":"Collinson","sequence":"additional","affiliation":[{"name":"Electron Microscopy Science Technology Platform, The Francis Crick Institute, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aoife","family":"Heaslip","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, University of Connecticut, Storrs, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4138-3055","authenticated-orcid":true,"given":"Gary E","family":"Ward","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, University of Vermont Larner College of Medicine, Burlington, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9727-6657","authenticated-orcid":true,"given":"Moritz","family":"Treeck","sequence":"additional","affiliation":[{"name":"Signalling in Apicomplexan Parasites Laboratory, The Francis Crick Institute, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4374","published-online":{"date-parts":[[2019,10,2]]},"reference":[{"key":"bib1","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1038\/nmeth.2301","article-title":"Conditional genome engineering in Toxoplasma gondii uncovers alternative invasion mechanisms","volume":"10","author":"Andenmatten","year":"2013","journal-title":"Nature Methods"},{"key":"bib2","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1080\/00034983.1995.11812940","article-title":"The role of the cytoskeleton in plasmodium falciparum merozoite biology: an electron-microscopic view","volume":"89","author":"Bannister","year":"1995","journal-title":"Annals of Tropical Medicine & Parasitology"},{"key":"bib3","doi-asserted-by":"publisher","first-page":"621","DOI":"10.1038\/nrmicro1465","article-title":"Regulation of apicomplexan actin-based motility","volume":"4","author":"Baum","year":"2006","journal-title":"Nature Reviews Microbiology"},{"key":"bib4","doi-asserted-by":"publisher","first-page":"2324","DOI":"10.1091\/mbc.e04-01-0048","article-title":"Cyclase-associated protein 1 (CAP1) promotes cofilin-induced actin dynamics in mammalian nonmuscle cells","volume":"15","author":"Bertling","year":"2004","journal-title":"Molecular Biology of the Cell"},{"key":"bib5","doi-asserted-by":"publisher","first-page":"9399","DOI":"10.1128\/MCB.20.24.9399-9408.2000","article-title":"Ionophore-resistant mutants of Toxoplasma gondii reveal host cell permeabilization as an early event in egress","volume":"20","author":"Black","year":"2000","journal-title":"Molecular and Cellular Biology"},{"key":"bib6","doi-asserted-by":"publisher","first-page":"607","DOI":"10.1128\/MMBR.64.3.607-623.2000","article-title":"Lytic cycle of Toxoplasma gondii","volume":"64","author":"Black","year":"2000","journal-title":"Microbiology and Molecular Biology Reviews"},{"key":"bib7","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00375-17","article-title":"Plasma membrane association by N-Acylation governs PKG function in Toxoplasma gondii","volume":"8","author":"Brown","year":"2017","journal-title":"mBio"},{"key":"bib8","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003331","article-title":"A nucleotide sugar transporter involved in glycosylation of the Toxoplasma tissue cyst wall is required for efficient persistence of bradyzoites","volume":"9","author":"Caffaro","year":"2013","journal-title":"PLOS Pathogens"},{"key":"bib9","article-title":"NCMIR methods for 3D EM: a new\u00a0protocol for preparation of biological specimens for serial block face scanning electron microscopy","author":"Deerinck","year":"2010","unstructured":"Deerinck TJ, Bushong EA, Thor A, Ellisman MH. 2010. NCMIR methods for 3D EM: a new\u00a0protocol for preparation of biological specimens for serial block face scanning electron microscopy. http:\/\/gatan.actonservice.com\/acton\/attachment\/11413\/f-017e\/1\/-\/-\/-\/-\/sbfsem%20sample%20prep%20protocol.pdf?modal=1 [Accessed November 21, 2018]."},{"key":"bib10","doi-asserted-by":"publisher","first-page":"613","DOI":"10.1083\/jcb.200012116","article-title":"Toxoplasma gondii myosins B\/C: one gene, Two tails, Two Localizations, and a role in parasite division","volume":"155","author":"Delbac","year":"2001","journal-title":"The Journal of Cell Biology"},{"key":"bib11","doi-asserted-by":"publisher","first-page":"253","DOI":"10.1002\/(SICI)1097-0169(1997)37:3<253::AID-CM7>3.0.CO;2-7","article-title":"Actin in the Toxoplasma gondii is encoded by a single copy gene,ACT1 and exists primarily in a globular form","volume":"37","author":"Dobrowolski","year":"1997","journal-title":"Cell Motility"},{"key":"bib12","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0091819","article-title":"The Toxoplasma Acto-MyoA motor complex is important but not essential for gliding motility and host cell invasion","volume":"9","author":"Egarter","year":"2014","journal-title":"PLOS ONE"},{"key":"bib13","doi-asserted-by":"publisher","first-page":"501","DOI":"10.1016\/j.chom.2015.09.006","article-title":"Global analysis of palmitoylated proteins in Toxoplasma gondii","volume":"18","author":"Foe","year":"2015","journal-title":"Cell Host & Microbe"},{"key":"bib14","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00393-18","article-title":"The Toxoplasma gondii active serine hydrolase 4 regulates parasite division and intravacuolar parasite architecture","volume":"3","author":"Foe","year":"2018","journal-title":"mSphere"},{"key":"bib15","doi-asserted-by":"publisher","first-page":"520","DOI":"10.1128\/EC.00357-08","article-title":"Efficient gene replacements in Toxoplasma gondii strains deficient for nonhomologous end joining","volume":"8","author":"Fox","year":"2009","journal-title":"Eukaryotic Cell"},{"key":"bib16","doi-asserted-by":"publisher","first-page":"1193","DOI":"10.1128\/EC.00297-10","article-title":"Type II Toxoplasma gondii KU80 knockout strains enable functional analysis of genes required for cyst development and latent infection","volume":"10","author":"Fox","year":"2011","journal-title":"Eukaryotic Cell"},{"key":"bib17","doi-asserted-by":"publisher","first-page":"645","DOI":"10.1038\/nrmicro.2017.86","article-title":"Gliding motility powers invasion and egress in apicomplexa","volume":"15","author":"Fr\u00e9nal","year":"2017","journal-title":"Nature Reviews Microbiology"},{"key":"bib18","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms15710","article-title":"Myosin-dependent cell-cell communication controls synchronicity of division in acute and chronic stages of Toxoplasma gondii","volume":"8","author":"Fr\u00e9nal","year":"2017","journal-title":"Nature Communications"},{"key":"bib19","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003049","article-title":"A forward genetic screen reveals that Calcium-dependent protein kinase 3 regulates egress in\u00a0Toxoplasma","volume":"11","author":"Garrison","year":"2012","journal-title":"PLOS Pathogens"},{"key":"bib20","doi-asserted-by":"publisher","first-page":"901","DOI":"10.1038\/nmeth.1515","article-title":"Chemical synthesis of the mouse mitochondrial genome","volume":"7","author":"Gibson","year":"2010","journal-title":"Nature Methods"},{"key":"bib21","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005388","article-title":"The conoid associated motor MyoH is indispensable for Toxoplasma gondii entry and exit from host cells","volume":"12","author":"Graindorge","year":"2016","journal-title":"PLOS Pathogens"},{"key":"bib22","doi-asserted-by":"publisher","first-page":"3001","DOI":"10.1091\/mbc.E14-10-1427","article-title":"Disassembly activity of actin-depolymerizing factor (ADF) is associated with distinct cellular processes in apicomplexan parasites","volume":"26","author":"Haase","year":"2015","journal-title":"Molecular Biology of the Cell"},{"key":"bib23","doi-asserted-by":"publisher","first-page":"615","DOI":"10.1128\/CMR.00005-17","article-title":"Toxoplasma effectors targeting host signaling and transcription","volume":"30","author":"Hakimi","year":"2017","journal-title":"Clinical Microbiology Reviews"},{"key":"bib24","first-page":"125","volume-title":"Handbook of Clinical Neurology","author":"Halonen","year":"2013"},{"key":"bib25","doi-asserted-by":"publisher","first-page":"2080","DOI":"10.1091\/mbc.E15-12-0824","article-title":"Myosin and actin filaments","volume":"27","author":"Heaslip","year":"2016","journal-title":"Molecular Biology of the Cell"},{"key":"bib26","doi-asserted-by":"publisher","first-page":"11572","DOI":"10.1074\/jbc.M109.054916","article-title":"Structure and function of a G-actin sequestering protein with a vital role in malaria oocyst development inside the mosquito vector","volume":"285","author":"Hliscs","year":"2010","journal-title":"Journal of Biological Chemistry"},{"key":"bib27","doi-asserted-by":"publisher","first-page":"1145","DOI":"10.1021\/cb501004q","article-title":"Identification of potent phosphodiesterase inhibitors that demonstrate cyclic nucleotide-dependent functions in apicomplexan parasites","volume":"10","author":"Howard","year":"2015","journal-title":"ACS Chemical Biology"},{"key":"bib28","doi-asserted-by":"publisher","first-page":"530","DOI":"10.1128\/EC.00358-08","article-title":"Tagging of endogenous genes in a Toxoplasma gondii strain lacking Ku80","volume":"8","author":"Huynh","year":"2009","journal-title":"Eukaryotic Cell"},{"key":"bib29","doi-asserted-by":"publisher","first-page":"1702","DOI":"10.1038\/emboj.2013.113","article-title":"Toxoplasma gondii myosin F, an essential motor for centrosomes positioning and apicoplast inheritance","volume":"32","author":"Jacot","year":"2013","journal-title":"The EMBO Journal"},{"key":"bib30","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0018556","article-title":"High cleavage efficiency of a 2A peptide derived from porcine teschovirus-1 in human cell lines, zebrafish and mice","volume":"6","author":"Kim","year":"2011","journal-title":"PLOS ONE"},{"key":"bib31","doi-asserted-by":"publisher","first-page":"8125","DOI":"10.1093\/nar\/15.20.8125","article-title":"An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs","volume":"15","author":"Kozak","year":"1987","journal-title":"Nucleic Acids Research"},{"key":"bib32","doi-asserted-by":"publisher","first-page":"947","DOI":"10.1016\/0022-2836(87)90418-9","article-title":"At least six nucleotides preceding the AUG initiator Codon enhance translation in mammalian cells","volume":"196","author":"Kozak","year":"1987","journal-title":"Journal of Molecular Biology"},{"key":"bib33","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1006\/jsbi.1996.0013","article-title":"Computer visualization of three-dimensional image data using IMOD","volume":"116","author":"Kremer","year":"1996","journal-title":"Journal of Structural Biology"},{"key":"bib34","doi-asserted-by":"publisher","first-page":"249","DOI":"10.1016\/0166-6851(91)90092-K","article-title":"Characterization of the protein contents of rhoptries and dense granules of Toxoplasma gondii tachyzoites by subcellular fractionation and monoclonal antibodies","volume":"45","author":"Leriche","year":"1991","journal-title":"Molecular and Biochemical Parasitology"},{"key":"bib35","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0085763","article-title":"Disruption of TgPHIL1 alters specific parameters of Toxoplasma gondii motility measured in a quantitative, three-dimensional live motility assay","volume":"9","author":"Leung","year":"2014","journal-title":"PLOS ONE"},{"key":"bib36","doi-asserted-by":"publisher","first-page":"1069","DOI":"10.1002\/cm.21077","article-title":"Targeted proteomic dissection of Toxoplasma cytoskeleton sub-compartments using MORN1","volume":"69","author":"Lorestani","year":"2012","journal-title":"Cytoskeleton"},{"key":"bib37","doi-asserted-by":"publisher","first-page":"4524","DOI":"10.1038\/emboj.2012.299","article-title":"Distinct signalling pathways control Toxoplasma egress and host-cell invasion","volume":"31","author":"Lourido","year":"2012","journal-title":"The EMBO Journal"},{"key":"bib38","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1002\/jemt.20182","article-title":"Intravacuolar network may act as a mechanical support for Toxoplasma gondii inside the parasitophorous vacuole","volume":"67","author":"Magno","year":"2005","journal-title":"Microscopy Research and Technique"},{"key":"bib39","doi-asserted-by":"publisher","first-page":"984","DOI":"10.1074\/jbc.M112.435719","article-title":"Mammalian and malaria parasite cyclase-associated proteins catalyze nucleotide exchange on G-actin through a conserved mechanism","volume":"288","author":"Makkonen","year":"2013","journal-title":"Journal of Biological Chemistry"},{"key":"bib40","doi-asserted-by":"publisher","first-page":"5158","DOI":"10.1091\/mbc.e04-06-0444","article-title":"A high-affinity interaction with ADP-actin monomers underlies the mechanism and in vivo function of Srv2\/cyclase-associated protein","volume":"15","author":"Mattila","year":"2004","journal-title":"Molecular Biology of the Cell"},{"key":"bib41","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003066","article-title":"TgCDPK3 regulates calcium-dependent egress of Toxoplasma gondii from host cells","volume":"8","author":"McCoy","year":"2012","journal-title":"PLOS Pathogens"},{"key":"bib42","doi-asserted-by":"publisher","first-page":"1290","DOI":"10.1091\/mbc.e10-12-0939","article-title":"Actin depolymerizing factor controls actin turnover and gliding motility in Toxoplasma gondii","volume":"22","author":"Mehta","year":"2011","journal-title":"Molecular Biology of the Cell"},{"key":"bib43","doi-asserted-by":"publisher","DOI":"10.1155\/2011\/473983","article-title":"Contribution of the residual body in the spatial organization of Toxoplasma gondii tachyzoites within the parasitophorous vacuole","volume":"2011","author":"Mu\u00f1iz-Hern\u00e1ndez","year":"2011","journal-title":"BioMed Research International"},{"key":"bib44","doi-asserted-by":"publisher","first-page":"861","DOI":"10.1093\/nar\/gkm1102","article-title":"Diversity of preferred nucleotide sequences around the translation initiation codon in eukaryote genomes","volume":"36","author":"Nakagawa","year":"2008","journal-title":"Nucleic Acids Research"},{"key":"bib45","doi-asserted-by":"publisher","first-page":"3249","DOI":"10.1242\/jcs.128231","article-title":"The role of cyclase-associated protein in regulating actin filament dynamics - more than a monomer-sequestration factor","volume":"126","author":"Ono","year":"2013","journal-title":"Journal of Cell Science"},{"key":"bib46","doi-asserted-by":"publisher","DOI":"10.1099\/mgen.0.000033","article-title":"EuPaGDT: a web tool tailored to design CRISPR guide RNAs for eukaryotic pathogens","volume":"1","author":"Peng","year":"2015","journal-title":"Microbial Genomics"},{"key":"bib47","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.24119","article-title":"Toxoplasma gondii F-actin forms an extensive filamentous network required for material exchange and parasite maturation","volume":"6","author":"Periz","year":"2017","journal-title":"eLife"},{"key":"bib48","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1016\/j.chom.2008.01.001","article-title":"Toxoplasma profilin is essential for host cell invasion and TLR11-dependent induction of an interleukin-12 response","volume":"3","author":"Plattner","year":"2008","journal-title":"Cell Host & Microbe"},{"key":"bib49","doi-asserted-by":"publisher","first-page":"10636","DOI":"10.1073\/pnas.1707506114","article-title":"Near-atomic structure of jasplakinolide-stabilized malaria parasite F-actin reveals the structural basis of filament instability","volume":"114","author":"Pospich","year":"2017","journal-title":"PNAS"},{"key":"bib50","doi-asserted-by":"publisher","first-page":"2424","DOI":"10.1091\/mbc.e10-12-0950","article-title":"Progressive sheet-to-tubule transformation is a general mechanism for endoplasmic reticulum partitioning in dividing mammalian cells","volume":"23","author":"Puhka","year":"2012","journal-title":"Molecular Biology of the Cell"},{"key":"bib51","doi-asserted-by":"publisher","first-page":"9625","DOI":"10.1073\/pnas.1015980108","article-title":"Polymorphic family of injected pseudokinases is paramount in Toxoplasma virulence","volume":"108","author":"Reese","year":"2011","journal-title":"PNAS"},{"key":"bib52","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0159306","article-title":"Phenotypes associated with knockouts of eight dense granule gene loci (GRA2-9) in virulent Toxoplasma gondii","volume":"11","author":"Rommereim","year":"2016","journal-title":"PLOS ONE"},{"key":"bib53","doi-asserted-by":"publisher","DOI":"10.1101\/646463","article-title":"Apicomplexan F-actin is required for efficient nuclear entry during host cell invasion","volume-title":"bioRxiv","author":"Rosario","year":"2019"},{"key":"bib54","doi-asserted-by":"publisher","first-page":"480","DOI":"10.1038\/nmeth.3372","article-title":"ClearVolume: open-source live 3D visualization for light-sheet microscopy","volume":"12","author":"Royer","year":"2015","journal-title":"Nature Methods"},{"key":"bib55","doi-asserted-by":"publisher","first-page":"278","DOI":"10.1242\/jcs.188433","article-title":"3d correlative light and electron microscopy of cultured cells using serial blockface scanning electron microscopy","volume":"130","author":"Russell","year":"2017","journal-title":"Journal of Cell Science"},{"key":"bib56","doi-asserted-by":"publisher","first-page":"895","DOI":"10.1091\/mbc.e05-06-0512","article-title":"Unusual kinetic and structural properties control rapid assembly and turnover of actin in the parasite Toxoplasma gondii","volume":"17","author":"Sahoo","year":"2006","journal-title":"Molecular Biology of the Cell"},{"key":"bib57","doi-asserted-by":"publisher","first-page":"4729","DOI":"10.1096\/fj.14-252569","article-title":"Structure of Toxoplasma gondii coronin, an actin-binding protein that relocalizes to the posterior pole of invasive parasites and contributes to invasion and egress","volume":"28","author":"Salamun","year":"2014","journal-title":"The FASEB Journal"},{"key":"bib58","doi-asserted-by":"publisher","first-page":"2234","DOI":"10.1091\/mbc.E15-10-0734","article-title":"Comparative Plasmodium gene overexpression reveals distinct perturbation of sporozoite transmission by profilin","volume":"27","author":"Sato","year":"2016","journal-title":"Molecular Biology of the Cell"},{"key":"bib59","doi-asserted-by":"publisher","first-page":"83","DOI":"10.1083\/jcb.201809107","article-title":"Dynamic nanoscale morphology of the ER surveyed by STED microscopy","volume":"218","author":"Schroeder","year":"2019","journal-title":"The Journal of Cell Biology"},{"key":"bib60","doi-asserted-by":"publisher","first-page":"309","DOI":"10.1007\/s004360050254","article-title":"Consensus sequence of translational initiation sites from Toxoplasma gondii genes","volume":"83","author":"Seeber","year":"1997","journal-title":"Parasitology Research"},{"key":"bib61","doi-asserted-by":"publisher","first-page":"9095","DOI":"10.1073\/pnas.96.16.9095","article-title":"Induction of an acrosomal process in Toxoplasma gondii: visualization of actin filaments in a protozoan parasite","volume":"96","author":"Shaw","year":"1999","journal-title":"PNAS"},{"key":"bib62","doi-asserted-by":"publisher","first-page":"209","DOI":"10.2307\/3276925","article-title":"The fine structure and reproduction of Toxoplasma gondii","volume":"54","author":"Sheffield","year":"1968","journal-title":"The Journal of Parasitology"},{"key":"bib63","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01114-14","article-title":"Efficient gene disruption in diverse strains of Toxoplasma gondii using CRISPR\/CAS9","volume":"5","author":"Shen","year":"2014","journal-title":"mBio"},{"key":"bib64","doi-asserted-by":"publisher","first-page":"234","DOI":"10.1016\/j.vetpar.2006.08.028","article-title":"Evaluation of serological tests for the diagnosis of Neospora caninum infection in dogs: optimization of cut off titers and inhibition studies of cross-reactivity with Toxoplasma gondii","volume":"143","author":"Silva","year":"2007","journal-title":"Veterinary Parasitology"},{"key":"bib65","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002280","article-title":"Evolutionarily divergent, unstable filamentous actin is essential for gliding motility in apicomplexan parasites","volume":"7","author":"Skillman","year":"2011","journal-title":"PLOS Pathogens"},{"key":"bib66","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.49030","article-title":"Formin-2 drives polymerisation of actin filaments enabling segregation of apicoplasts and cytokinesis in plasmodium falciparum","volume":"8","author":"Stortz","year":"2019","journal-title":"eLife"},{"key":"bib67","doi-asserted-by":"publisher","first-page":"254","DOI":"10.1080\/10409238.2017.1290043","article-title":"Genomics of apicomplexan parasites","volume":"52","author":"Swapna","year":"2017","journal-title":"Critical Reviews in Biochemistry and Molecular Biology"},{"key":"bib68","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003629","article-title":"Protein trafficking through the endosomal system prepares intracellular parasites for a home invasion","volume":"9","author":"Tomavo","year":"2013","journal-title":"PLOS Pathogens"},{"key":"bib69","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.42669","article-title":"Three F-actin assembly centers regulate organelle inheritance, cell-cell communication and motility in Toxoplasma gondii","volume":"8","author":"Tosetti","year":"2019","journal-title":"eLife"},{"key":"bib70","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004197","article-title":"The calcium-dependent protein kinase 3 of Toxoplasma influences basal calcium levels and functions beyond egress as revealed by quantitative phosphoproteome analysis","volume":"10","author":"Treeck","year":"2014","journal-title":"PLOS Pathogens"},{"key":"bib71","doi-asserted-by":"publisher","first-page":"1380","DOI":"10.1111\/j.1365-2958.2006.05343.x","article-title":"The cell biology of secondary endosymbiosis ? how parasites build, divide and segregate the apicoplast","volume":"61","author":"Vaishnava","year":"2006","journal-title":"Molecular Microbiology"},{"key":"bib72","doi-asserted-by":"publisher","first-page":"333","DOI":"10.1083\/jcb.201011039","article-title":"A 3D analysis of yeast ER structure reveals how ER domains are organized by membrane curvature","volume":"193","author":"West","year":"2011","journal-title":"The Journal of Cell Biology"},{"key":"bib73","doi-asserted-by":"publisher","DOI":"10.1186\/s12915-016-0343-5","article-title":"Surface attachment, promoted by the actomyosin system of Toxoplasma gondii is important for efficient gliding motility and invasion","volume":"15","author":"Whitelaw","year":"2017","journal-title":"BMC Biology"}],"container-title":["eLife"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/cdn.elifesciences.org\/articles\/50598\/elife-50598-v2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/cdn.elifesciences.org\/articles\/50598\/elife-50598-v2.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/elifesciences.org\/articles\/50598","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T21:04:00Z","timestamp":1697058240000},"score":1,"resource":{"primary":{"URL":"https:\/\/elifesciences.org\/articles\/50598"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,10,2]]},"references-count":73,"alternative-id":["10.7554\/eLife.50598"],"URL":"https:\/\/doi.org\/10.7554\/elife.50598","archive":["CLOCKSS"],"relation":{"is-supplemented-by":[{"id-type":"accession","id":"EMPIAR-10324","asserted-by":"subject"},{"id-type":"accession","id":"EMPIAR-10325","asserted-by":"subject"},{"id-type":"accession","id":"EMPIAR-10326","asserted-by":"subject"},{"id-type":"accession","id":"EMPIAR-10327","asserted-by":"subject"}],"has-review":[{"id-type":"doi","id":"10.7554\/eLife.50598.050","asserted-by":"object"},{"id-type":"doi","id":"10.7554\/eLife.50598.051","asserted-by":"object"}]},"ISSN":["2050-084X"],"issn-type":[{"value":"2050-084X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,10,2]]},"article-number":"e50598"}}