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Here, we describe a broadly applicable strategy, based on llama-derived single-domain antibodies (VHHs), for the development of bunyavirus biotherapeutics. The method was validated using the zoonotic Rift Valley fever virus (RVFV) and Schmallenberg virus (SBV), an emerging pathogen of ruminants, as model pathogens. VHH building blocks were assembled into highly potent neutralizing complexes using bacterial superglue technology. The multimeric complexes were shown to reduce and prevent virus-induced morbidity and mortality in mice upon prophylactic administration. Bispecific molecules engineered to present two different VHHs fused to an Fc domain were further shown to be effective upon therapeutic administration. The presented VHH-based technology holds great promise for the development of bunyavirus antiviral therapies.<\/jats:p>","DOI":"10.7554\/elife.52716","type":"journal-article","created":{"date-parts":[[2020,4,21]],"date-time":"2020-04-21T10:00:12Z","timestamp":1587463212000},"source":"Crossref","is-referenced-by-count":41,"title":["Multimeric single-domain antibody complexes protect against bunyavirus infections"],"prefix":"10.7554","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9790-2438","authenticated-orcid":true,"given":"Paul J","family":"Wichgers Schreur","sequence":"first","affiliation":[{"name":"Department of Virology, Wageningen Bioveterinary Research, Lelystad, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"van de Water","sequence":"additional","affiliation":[{"name":"Department of Virology, Wageningen Bioveterinary Research, Lelystad, 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