{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T19:25:46Z","timestamp":1792092346854,"version":"4.4.0"},"reference-count":85,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2020,4,21]],"date-time":"2020-04-21T00:00:00Z","timestamp":1587427200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,4,21]],"date-time":"2020-04-21T00:00:00Z","timestamp":1587427200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,4,21]],"date-time":"2020-04-21T00:00:00Z","timestamp":1587427200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000274","name":"British Heart Foundation","doi-asserted-by":"publisher","award":["FS\/15\/65\/32036"],"award-info":[{"award-number":["FS\/15\/65\/32036"]}],"id":[{"id":"10.13039\/501100000274","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000274","name":"British Heart Foundation","doi-asserted-by":"publisher","award":["PG\/17\/22\/32868"],"award-info":[{"award-number":["PG\/17\/22\/32868"]}],"id":[{"id":"10.13039\/501100000274","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000274","name":"British Heart Foundation","doi-asserted-by":"publisher","award":["FS\/18\/53\/33863"],"award-info":[{"award-number":["FS\/18\/53\/33863"]}],"id":[{"id":"10.13039\/501100000274","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elifesciences.org"],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>\n                    Platelet-neutrophil interactions are important for innate immunity, but also contribute to the pathogenesis of deep vein thrombosis, myocardial infarction and stroke. Here we report that, under flow, von Willebrand factor\/glycoprotein Ib\u03b1-dependent platelet \u2018priming\u2019 induces integrin \u03b1\n                    <jats:sub>IIb<\/jats:sub>\n                    \u03b2\n                    <jats:sub>3<\/jats:sub>\n                    activation that, in turn, mediates neutrophil and T-cell binding. Binding of platelet \u03b1\n                    <jats:sub>IIb<\/jats:sub>\n                    \u03b2\n                    <jats:sub>3<\/jats:sub>\n                    to SLC44A2 on neutrophils leads to mechanosensitive-dependent production of highly prothrombotic neutrophil extracellular traps. A polymorphism in\n                    <jats:italic>SLC44A2<\/jats:italic>\n                    (rs2288904-A) present in 22% of the population causes an R154Q substitution in an extracellular loop of SLC44A2 that is protective against venous thrombosis results in severely impaired binding to both activated \u03b1\n                    <jats:sub>IIb<\/jats:sub>\n                    \u03b2\n                    <jats:sub>3<\/jats:sub>\n                    and VWF-primed platelets. This was confirmed using neutrophils homozygous for the\n                    <jats:italic>SLC44A2<\/jats:italic>\n                    R154Q polymorphism. Taken together, these data reveal a previously unreported mode of platelet-neutrophil crosstalk, mechanosensitive NET production, and provide mechanistic insight into the protective effect of the\n                    <jats:italic>SLC44A2<\/jats:italic>\n                    rs2288904-A polymorphism in venous thrombosis.\n                  <\/jats:p>","DOI":"10.7554\/elife.53353","type":"journal-article","created":{"date-parts":[[2020,4,21]],"date-time":"2020-04-21T08:00:18Z","timestamp":1587456018000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.53353","source":"Crossref","is-referenced-by-count":107,"title":["Activated \u03b1IIb\u03b23 on platelets mediates flow-dependent NETosis via SLC44A2"],"prefix":"10.7554","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1274-2867","authenticated-orcid":true,"given":"Adela","family":"Constantinescu-Bercu","sequence":"first","affiliation":[{"name":"Centre for Haematology, Department of Immunology and Inflammation, Imperial College London, London, United Kingdom"},{"name":"Centre for Inflammatory Disease, Department of Immunology and Inflammation, Imperial College London, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luigi","family":"Grassi","sequence":"additional","affiliation":[{"name":"Department of Haematology, University of Cambridge, Cambridge Biomedical Campus, Cambridge, United Kingdom"},{"name":"National Health Service Blood and Transplant, Cambridge Biomedical Campus, Cambridge, United Kingdom"},{"name":"National Institute for Health Research BioResource, Rare Diseases, Cambridge University Hospitals, Cambridge, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mattia","family":"Frontini","sequence":"additional","affiliation":[{"name":"Department of Haematology, University of Cambridge, Cambridge Biomedical Campus, Cambridge, United Kingdom"},{"name":"National Health Service Blood and Transplant, Cambridge Biomedical Campus, Cambridge, United Kingdom"},{"name":"British Heart Foundation Centre of Excellence, Cambridge Biomedical Campus, Cambridge, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle I","family":"Salles-Crawley","sequence":"additional","affiliation":[{"name":"Centre for Haematology, Department of Immunology and Inflammation, Imperial College London, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9839-5463","authenticated-orcid":true,"given":"Kevin","family":"Woollard","sequence":"additional","affiliation":[{"name":"Centre for Inflammatory Disease, Department of Immunology and Inflammation, Imperial College London, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6723-7841","authenticated-orcid":true,"given":"James TB","family":"Crawley","sequence":"additional","affiliation":[{"name":"Centre for Haematology, 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