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Here, we demonstrate that Zcwpw1 is an H3K4me3 reader that is required for DSB repair and synapsis in mouse testes. We generated H3K4me3 reader-dead Zcwpw1 mutant mice and found that their spermatocytes were arrested at the pachytene-like stage, which phenocopies the\n                    <jats:italic>Zcwpw1<\/jats:italic>\n                    knock\u2013out mice. Based on various ChIP-seq and immunofluorescence analyses using several mutants, we found that Zcwpw1's occupancy on chromatin is strongly promoted by the histone-modification activity of PRDM9. Zcwpw1 localizes to DMC1-labelled hotspots in a largely Prdm9-dependent manner, where it facilitates completion of synapsis by mediating the DSB repair process. In sum, our study demonstrates the function of ZCWPW1 that acts as part of the selection system for epigenetics-based recombination hotspots in mammals.\n                  <\/jats:p>","DOI":"10.7554\/elife.53459","type":"journal-article","created":{"date-parts":[[2020,5,6]],"date-time":"2020-05-06T08:00:14Z","timestamp":1588752014000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.53459","source":"Crossref","is-referenced-by-count":62,"title":["The histone modification reader ZCWPW1 links histone methylation to PRDM9-induced double-strand break repair"],"prefix":"10.7554","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7086-570X","authenticated-orcid":true,"given":"Tao","family":"Huang","sequence":"first","affiliation":[{"name":"Center for Reproductive Medicine, Cheeloo College of Medicine, Shandong University, Jinan, China"},{"name":"National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Shandong University, Jinan, 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