{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T23:17:56Z","timestamp":1790291876608,"version":"4.1.0"},"reference-count":90,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2020,3,16]],"date-time":"2020-03-16T00:00:00Z","timestamp":1584316800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,3,16]],"date-time":"2020-03-16T00:00:00Z","timestamp":1584316800000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,3,16]],"date-time":"2020-03-16T00:00:00Z","timestamp":1584316800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000011","name":"Howard Hughes Medical Institute","doi-asserted-by":"publisher","award":["Allan Spradling"],"award-info":[{"award-number":["Allan Spradling"]}],"id":[{"id":"10.13039\/100000011","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Adult Drosophila Malpighian tubules have low rates of cell turnover but are vulnerable to damage caused by stones, like their mammalian counterparts, kidneys. We show that Drosophilarenal stem cells (RSCs) in the ureter and lower tubules comprise a unique, unipotent regenerative compartment. RSCs respond only to loss of nearby principal cells (PCs), cells critical for maintaining ionic balance. Large polyploid PCs are outnumbered by RSCs, which replace each lost cell with multiple PCs of lower ploidy. Notably, RSCs do not replenish principal cells or stellate cells in the upper tubules. RSCs generate daughters by asymmetric Notch signaling, yet RSCs remain quiescent (cell cycle-arrested) without damage. Nevertheless, the capacity for RSC-mediated repair extends the lifespan of flies carrying kidney stones. We propose that abundant, RSC-like stem cells exist in other tissues with low rates of turnover where they may have been mistaken for differentiated tissue cells.<\/jats:p>","DOI":"10.7554\/elife.54096","type":"journal-article","created":{"date-parts":[[2020,3,16]],"date-time":"2020-03-16T09:00:20Z","timestamp":1584349220000},"source":"Crossref","is-referenced-by-count":32,"title":["An abundant quiescent stem cell population in Drosophila Malpighian tubules protects principal cells from kidney stones"],"prefix":"10.7554","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7408-234X","authenticated-orcid":true,"given":"Chenhui","family":"Wang","sequence":"first","affiliation":[{"name":"Howard Hughes Medical Institute Research Laboratories, Department of Embryology, Carnegie Institution for Science, Baltimore, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5251-1801","authenticated-orcid":true,"given":"Allan C","family":"Spradling","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute Research Laboratories, Department of Embryology, Carnegie Institution for Science, Baltimore, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4374","published-online":{"date-parts":[[2020,3,16]]},"reference":[{"key":"bib1","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1002\/gene.10110","article-title":"Puckered-GAL4 driving in JNK-active cells","volume":"34","author":"Adachi-Yamada","year":"2002","journal-title":"Genesis"},{"key":"bib2","doi-asserted-by":"publisher","first-page":"274","DOI":"10.5152\/tud.2013.066","article-title":"Classical xanthinuria: a rare cause of pediatric urolithiasis","volume":"39","author":"Ak\u0131nc\u0131","year":"2013","journal-title":"T\u00fcrk \u00dcroloji Dergisi\/Turkish Journal of Urology"},{"key":"bib3","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1016\/j.stem.2008.10.016","article-title":"Tissue damage-induced intestinal stem cell division inDrosophila Cell","volume":"4","author":"Amcheslavsky","year":"2009","journal-title":"Stem Cell"},{"key":"bib4","doi-asserted-by":"publisher","first-page":"1714","DOI":"10.1002\/stem.1130","article-title":"Characterization of renal progenitors committed toward tubular lineage and their regenerative potential in renal tubular injury","volume":"30","author":"Angelotti","year":"2012","journal-title":"Stem Cells"},{"key":"bib5","doi-asserted-by":"publisher","first-page":"323","DOI":"10.1016\/j.modgep.2006.08.003","article-title":"GFP reporters detect the activation of the Drosophila JAK\/STAT pathway in vivo","volume":"7","author":"Bach","year":"2007","journal-title":"Gene Expression Patterns"},{"key":"bib6","doi-asserted-by":"publisher","first-page":"442","DOI":"10.1016\/j.stem.2008.07.024","article-title":"JNK activity in somatic stem cells causes loss of tissue homeostasis in the aging Drosophila gut","volume":"3","author":"Biteau","year":"2008","journal-title":"Cell Stem Cell"},{"key":"bib7","first-page":"1027","article-title":"[Studies on the chemical nature and formation of the urinary conglomerate in the malpighian vessels of the rosy mutant of Drosophila melanogaster]","volume":"22","author":"Bonse","year":"1967","journal-title":"Zeitschrift Fur Naturforschung. 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