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However mechanisms that maintain or compromise choroidal homeostasis are obscure. We discovered that the ablation of choroidal macrophages via CSF1R blockade was associated with choroidal vascular atrophy and retinal pigment epithelial (RPE) changes including structural disruption, downregulation of visual cycle genes, and altered angiogenic factor expression. Suspending CSF1R blockade following ablation enabled spontaneous macrophage regeneration, which fully restored original macrophage distributions and morphologies. Macrophage regeneration was accompanied by arrested vascular degeneration and ameliorated pathological RPE alterations. These findings suggest that choroidal macrophages play a previously unappreciated trophic role in maintaining choroidal vasculature and RPE cells, implicating insufficiency in choroidal macrophage function as a factor in aging- and AMD-associated pathology. Modulating macrophage function may constitute a strategy for the therapeutic preservation of the choroid and RPE in age-related retinal disorders.<\/jats:p>","DOI":"10.7554\/elife.55564","type":"journal-article","created":{"date-parts":[[2020,4,1]],"date-time":"2020-04-01T08:00:18Z","timestamp":1585728018000},"source":"Crossref","is-referenced-by-count":47,"title":["CSF1R blockade induces macrophage ablation and results in mouse choroidal vascular atrophy and RPE disorganization"],"prefix":"10.7554","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0429-9188","authenticated-orcid":true,"given":"Xiao","family":"Yang","sequence":"first","affiliation":[{"name":"Section on Neuron-Glia Interactions in Retinal Disease, National Eye Institute, National Institutes of Health, Bethesda, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lian","family":"Zhao","sequence":"additional","affiliation":[{"name":"Section on Neuron-Glia Interactions in Retinal 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