{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T14:56:15Z","timestamp":1790952975682,"version":"4.1.0"},"reference-count":85,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2020,11,19]],"date-time":"2020-11-19T00:00:00Z","timestamp":1605744000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"},{"start":{"date-parts":[[2020,11,19]],"date-time":"2020-11-19T00:00:00Z","timestamp":1605744000000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"},{"start":{"date-parts":[[2020,11,19]],"date-time":"2020-11-19T00:00:00Z","timestamp":1605744000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"funder":[{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["ZIA BC010338-19"],"award-info":[{"award-number":["ZIA BC010338-19"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elifesciences.org"],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>\n                    During vertebrate development, the presomitic mesoderm (PSM) periodically segments into somites, which will form the segmented vertebral column and associated muscle, connective tissue, and dermis. The periodicity of somitogenesis is regulated by a segmentation clock of oscillating Notch activity. Here, we examined mouse mutants lacking only\n                    <jats:italic>Fgf4<\/jats:italic>\n                    or\n                    <jats:italic>Fgf8<\/jats:italic>\n                    , which we previously demonstrated act redundantly to prevent PSM differentiation.\n                    <jats:italic>Fgf8<\/jats:italic>\n                    is not required for somitogenesis, but\n                    <jats:italic>Fgf4<\/jats:italic>\n                    mutants display a range of vertebral defects. We analyzed\n                    <jats:italic>Fgf4<\/jats:italic>\n                    mutants by quantifying mRNAs fluorescently labeled by hybridization chain reaction within Imaris-based volumetric tissue subsets. These data indicate that FGF4 maintains\n                    <jats:italic>Hes7<\/jats:italic>\n                    levels and normal oscillatory patterns. To support our hypothesis that FGF4 regulates somitogenesis through\n                    <jats:italic>Hes7<\/jats:italic>\n                    , we demonstrate genetic synergy between\n                    <jats:italic>Hes7<\/jats:italic>\n                    and\n                    <jats:italic>Fgf4<\/jats:italic>\n                    , but not with\n                    <jats:italic>Fgf8<\/jats:italic>\n                    . Our data indicate that\n                    <jats:italic>Fgf4<\/jats:italic>\n                    is potentially important in a spectrum of human Segmentation Defects of the Vertebrae caused by defective Notch oscillations.\n                  <\/jats:p>","DOI":"10.7554\/elife.55608","type":"journal-article","created":{"date-parts":[[2020,11,19]],"date-time":"2020-11-19T08:00:51Z","timestamp":1605772851000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.55608","source":"Crossref","is-referenced-by-count":60,"title":["Fgf4 maintains Hes7 levels critical for normal somite segmentation clock function"],"prefix":"10.7554","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9387-5743","authenticated-orcid":true,"given":"Matthew J","family":"Anderson","sequence":"first","affiliation":[{"name":"Genetics of Vertebrate Development Section, Cancer and Developmental Biology Laboratory, National Cancer Institute, National Institutes of Health, Frederick, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentin","family":"Magidson","sequence":"additional","affiliation":[{"name":"Optical Microscopy and Analysis Laboratory, Frederick National Laboratory for Cancer Research, Frederick, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryoichiro","family":"Kageyama","sequence":"additional","affiliation":[{"name":"Institute for Frontier Life and Medical Sciences, Kyoto University, Kyoto, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1066-3735","authenticated-orcid":true,"given":"Mark","family":"Lewandoski","sequence":"additional","affiliation":[{"name":"Genetics of Vertebrate Development Section, Cancer and Developmental Biology Laboratory, National Cancer Institute, National Institutes of Health, Frederick, United 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