{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T11:09:48Z","timestamp":1790680188001,"version":"4.1.0"},"reference-count":99,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2020,9,8]],"date-time":"2020-09-08T00:00:00Z","timestamp":1599523200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,9,8]],"date-time":"2020-09-08T00:00:00Z","timestamp":1599523200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,9,8]],"date-time":"2020-09-08T00:00:00Z","timestamp":1599523200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R35GM122461"],"award-info":[{"award-number":["R35GM122461"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elifesciences.org"],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>\n                    Bacteria surround themselves with peptidoglycan, an adaptable enclosure that contributes to cell shape and stability. Peptidoglycan assembly relies on penicillin-binding proteins (PBPs) acting in concert with SEDS-family transglycosylases RodA and FtsW, which support cell elongation and division respectively. In\n                    <jats:italic>Bacillus subtilis<\/jats:italic>\n                    , cells lacking all four PBPs with transglycosylase activity (aPBPs) are viable. Here, we show that the alternative sigma factor \u03c3\n                    <jats:sup>I<\/jats:sup>\n                    is essential in the absence of aPBPs. Defects in aPBP-dependent wall synthesis are compensated by \u03c3\n                    <jats:sup>I<\/jats:sup>\n                    -dependent upregulation of an MreB homolog, MreBH, which localizes the LytE autolysin to the RodA-containing elongasome complex. Suppressor analysis reveals that cells unable to activate this \u03c3\n                    <jats:sup>I<\/jats:sup>\n                    stress response acquire gain-of-function mutations in the essential histidine kinase WalK, which also elevates expression of\n                    <jats:italic>sigI<\/jats:italic>\n                    ,\n                    <jats:italic>mreBH<\/jats:italic>\n                    and\n                    <jats:italic>lytE<\/jats:italic>\n                    . These results reveal compensatory mechanisms that balance the directional peptidoglycan synthesis arising from the elongasome complex with the more diffusive action of aPBPs.\n                  <\/jats:p>","DOI":"10.7554\/elife.57902","type":"journal-article","created":{"date-parts":[[2020,9,8]],"date-time":"2020-09-08T12:00:25Z","timestamp":1599566425000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.57902","source":"Crossref","is-referenced-by-count":53,"title":["A regulatory pathway that selectively up-regulates elongasome function in the absence of class A PBPs"],"prefix":"10.7554","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9888-9888","authenticated-orcid":true,"given":"Yesha","family":"Patel","sequence":"first","affiliation":[{"name":"Department of Microbiology, Cornell University, Ithaca, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7322-5513","authenticated-orcid":true,"given":"Heng","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Cornell University, Ithaca, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3832-3249","authenticated-orcid":true,"given":"John D","family":"Helmann","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Cornell University, Ithaca, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4374","published-online":{"date-parts":[[2020,9,8]]},"reference":[{"key":"bib1","doi-asserted-by":"publisher","first-page":"5421","DOI":"10.1128\/AEM.01453-16","article-title":"Editing of the Bacillus subtilis genome by the CRISPR-Cas9 system","volume":"82","author":"Altenbuchner","year":"2016","journal-title":"Applied and Environmental 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