{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T01:57:34Z","timestamp":1789610254520,"version":"build-2803163510"},"reference-count":33,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2020,12,7]],"date-time":"2020-12-07T00:00:00Z","timestamp":1607299200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,12,7]],"date-time":"2020-12-07T00:00:00Z","timestamp":1607299200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,12,7]],"date-time":"2020-12-07T00:00:00Z","timestamp":1607299200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["RO1 NS03538"],"award-info":[{"award-number":["RO1 NS03538"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006668","name":"Oregon Health and Science University","doi-asserted-by":"publisher","award":["NIH RO1 NS053538"],"award-info":[{"award-number":["NIH RO1 NS053538"]}],"id":[{"id":"10.13039\/100006668","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elifesciences.org"],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>\n                    Astrocytes exhibit spatially-restricted near-membrane microdomain Ca\n                    <jats:sup>2+<\/jats:sup>\n                    transients in their fine processes. How these transients are generated and regulate brain function in vivo remains unclear. Here we show that\n                    <jats:italic>Drosophila<\/jats:italic>\n                    astrocytes exhibit spontaneous, activity-independent microdomain Ca\n                    <jats:sup>2+<\/jats:sup>\n                    transients in their fine processes. Astrocyte microdomain Ca\n                    <jats:sup>2+<\/jats:sup>\n                    transients are mediated by the TRP channel TrpML, stimulated by reactive oxygen species (ROS), and can be enhanced in frequency by the neurotransmitter tyramine via the TyrRII receptor. Interestingly, many astrocyte microdomain Ca\n                    <jats:sup>2+<\/jats:sup>\n                    transients are closely associated with tracheal elements, which dynamically extend filopodia throughout the central nervous system (CNS) to deliver O\n                    <jats:sub>2<\/jats:sub>\n                    and regulate gas exchange. Many astrocyte microdomain Ca\n                    <jats:sup>2+<\/jats:sup>\n                    transients are spatio-temporally correlated with the initiation of tracheal filopodial retraction. Loss of TrpML leads to increased tracheal filopodial numbers, growth, and increased CNS ROS. We propose that local ROS production can activate astrocyte microdomain Ca\n                    <jats:sup>2+<\/jats:sup>\n                    transients through TrpML, and that a subset of these microdomain transients promotes tracheal filopodial retraction and in turn modulate CNS gas exchange.\n                  <\/jats:p>","DOI":"10.7554\/elife.58952","type":"journal-article","created":{"date-parts":[[2020,12,7]],"date-time":"2020-12-07T12:06:04Z","timestamp":1607342764000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.58952","source":"Crossref","is-referenced-by-count":33,"title":["TrpML-mediated astrocyte microdomain Ca2+ transients regulate astrocyte\u2013tracheal interactions"],"prefix":"10.7554","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2250-1861","authenticated-orcid":true,"given":"Zhiguo","family":"Ma","sequence":"first","affiliation":[{"name":"Vollum Institute, Oregon Health and Science University, Portland, United 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