{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T11:19:23Z","timestamp":1790421563945,"version":"4.1.0"},"reference-count":54,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2020,9,23]],"date-time":"2020-09-23T00:00:00Z","timestamp":1600819200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"},{"start":{"date-parts":[[2020,9,23]],"date-time":"2020-09-23T00:00:00Z","timestamp":1600819200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"},{"start":{"date-parts":[[2020,9,23]],"date-time":"2020-09-23T00:00:00Z","timestamp":1600819200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["1ZIADC000021"],"award-info":[{"award-number":["1ZIADC000021"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JSPS Research Fellowship for Japanese Biomedical and Behavioral Researchers at NIH"],"award-info":[{"award-number":["JSPS Research Fellowship for Japanese Biomedical and Behavioral Researchers at NIH"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elifesciences.org"],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>\n                    Directional sensitivity of hair cells (HCs) is conferred by the aymmetric apical hair bundle, comprised of a kinocilium and stereocilia staircase. The mother centriole (MC) forms the base of the kinocilium and the stereocilia develop adjacent to it. Previously, we showed that transcription factor Emx2 reverses hair bundle orientation and its expression in the mouse vestibular utricle is restricted, resulting in two regions of opposite bundle orientation (Jiang et al., 2017). Here, we investigated establishment of opposite bundle orientation in embryonic utricles by live-imaging GFP-labeled centrioles in HCs. The daughter centriole invariably migrated ahead of the MC from the center to their respective peripheral locations in HCs. Comparing HCs between utricular regions, centriole trajectories were similar but they migrated toward opposite directions, suggesting that Emx2 pre-patterned HCs prior to centriole migration. Ectopic\n                    <jats:italic>Emx2<\/jats:italic>\n                    , however, reversed centriole trajectory within hours during a critical time-window when centriole trajectory was responsive to Emx2.\n                  <\/jats:p>","DOI":"10.7554\/elife.59282","type":"journal-article","created":{"date-parts":[[2020,9,23]],"date-time":"2020-09-23T08:00:29Z","timestamp":1600848029000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.59282","source":"Crossref","is-referenced-by-count":18,"title":["Live imaging of hair bundle polarity acquisition demonstrates a critical timeline for transcription factor Emx2"],"prefix":"10.7554","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6868-8057","authenticated-orcid":true,"given":"Yosuke","family":"Tona","sequence":"first","affiliation":[{"name":"National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1400-3558","authenticated-orcid":true,"given":"Doris K","family":"Wu","sequence":"additional","affiliation":[{"name":"National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4374","published-online":{"date-parts":[[2020,9,23]]},"reference":[{"key":"bib1","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1101\/gad.1596308","article-title":"beta-Catenin is a Nek2 substrate involved in centrosome separation","volume":"22","author":"Bahmanyar","year":"2008","journal-title":"Genes & Development"},{"key":"bib2","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1007\/978-1-4939-3615-1_1","article-title":"Helios(R) Gene Gun-Mediated transfection of the inner ear sensory epithelium: recent 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