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Previously, we showed that transcription factor Emx2, expressed in only one of the daughter HCs, generates this bidirectional HC pattern (Jiang et al., 2017). Here, we asked whether Emx2 mediates this effect by changing location of hair bundle establishment or positions of HCs since daughter HCs are known to switch positions with each other. We showed this HC rearrangement, redefined as two processes named Rock and Roll, is required for positional acquisition of HCs. Apical protrusion formation of nascent HCs and planar polarity signaling are both important for the Rock and Roll. Emx2 facilitates Rock and Roll by delaying apical protrusion of its nascent HCs but it does not determine HCs\u2019 ultimate positions, indicating that Emx2 mediates bidirectional HC pattern by changing the location where hair bundle is established in HCs.<\/jats:p>","DOI":"10.7554\/elife.60432","type":"journal-article","created":{"date-parts":[[2020,12,30]],"date-time":"2020-12-30T08:00:26Z","timestamp":1609315226000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.60432","source":"Crossref","is-referenced-by-count":23,"title":["Emx2 regulates hair cell rearrangement but not positional identity within neuromasts"],"prefix":"10.7554","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2421-8093","authenticated-orcid":true,"given":"Sho","family":"Ohta","sequence":"first","affiliation":[{"name":"National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, United 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