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The transcription factor Myogenin (Myog) regulates myocyte fusion during development, but its role in adult myogenesis remains unclear. In contrast to mice,\n                    <jats:italic>\n                      myog\n                      <jats:sup>-\/-<\/jats:sup>\n                    <\/jats:italic>\n                    zebrafish are viable, but have hypotrophic muscles. By isolating adult myofibres with associated MuSCs, we found that\n                    <jats:italic>myog<\/jats:italic>\n                    <jats:sup>-\/-<\/jats:sup>\n                    myofibres have severely reduced nuclear number, but increased myonuclear domain size. Expression of fusogenic genes is decreased, Pax7 upregulated, MuSCs are fivefold more numerous and mis-positioned throughout the length of\n                    <jats:italic>\n                      myog\n                      <jats:sup>-\/-<\/jats:sup>\n                    <\/jats:italic>\n                    myofibres instead of localising at myofibre ends as in wild-type. Loss of Myog dysregulates mTORC1 signalling, resulting in an \u2018alerted\u2019 state of MuSCs, which display precocious activation and faster cell cycle entry ex vivo, concomitant with\n                    <jats:italic>myod<\/jats:italic>\n                    upregulation. Thus, beyond controlling myocyte fusion, Myog influences the MuSC:niche relationship, demonstrating a multi-level contribution to muscle homeostasis throughout life.\n                  <\/jats:p>","DOI":"10.7554\/elife.60445","type":"journal-article","created":{"date-parts":[[2020,10,1]],"date-time":"2020-10-01T08:00:59Z","timestamp":1601539259000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.60445","source":"Crossref","is-referenced-by-count":147,"title":["Myogenin is an essential regulator of adult myofibre growth and muscle stem cell homeostasis"],"prefix":"10.7554","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3163-9707","authenticated-orcid":true,"given":"Massimo","family":"Ganassi","sequence":"first","affiliation":[{"name":"Randall Centre for Cell and Molecular Biophysics, King\u2019s College London, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8407-8336","authenticated-orcid":true,"given":"Sara","family":"Badodi","sequence":"additional","affiliation":[{"name":"Blizard Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3209-9853","authenticated-orcid":true,"given":"Kees","family":"Wanders","sequence":"additional","affiliation":[{"name":"Randall Centre for Cell and Molecular Biophysics, King\u2019s College London, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9562-3072","authenticated-orcid":true,"given":"Peter S","family":"Zammit","sequence":"additional","affiliation":[{"name":"Randall Centre for Cell and Molecular Biophysics, King\u2019s College London, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8227-9225","authenticated-orcid":true,"given":"Simon M","family":"Hughes","sequence":"additional","affiliation":[{"name":"Randall Centre for Cell and Molecular Biophysics, King\u2019s College London, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4374","published-online":{"date-parts":[[2020,10,1]]},"reference":[{"key":"bib1","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1002\/ar.1091790109","article-title":"Distribution of mitotic nuclei in the biceps brachii of the mouse during post-natal growth","volume":"179","author":"Aziz","year":"1974","journal-title":"The Anatomical Record"},{"key":"bib2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.06541","article-title":"In vivo dynamics of skeletal muscle dystrophin in zebrafish embryos revealed by improved FRAP 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