{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T02:31:58Z","timestamp":1790649118944,"version":"4.1.0"},"reference-count":59,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2020,10,13]],"date-time":"2020-10-13T00:00:00Z","timestamp":1602547200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,10,13]],"date-time":"2020-10-13T00:00:00Z","timestamp":1602547200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,10,13]],"date-time":"2020-10-13T00:00:00Z","timestamp":1602547200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000272","name":"National Institute for Health Research","doi-asserted-by":"publisher","award":["AI 132378"],"award-info":[{"award-number":["AI 132378"]}],"id":[{"id":"10.13039\/501100000272","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000854","name":"Human Frontier Science Program","doi-asserted-by":"publisher","award":["RGY0066\/2016"],"award-info":[{"award-number":["RGY0066\/2016"]}],"id":[{"id":"10.13039\/501100000854","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004440","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["100993\/Z\/13\/Z"],"award-info":[{"award-number":["100993\/Z\/13\/Z"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004440","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["109007\/Z\/15\/A"],"award-info":[{"award-number":["109007\/Z\/15\/A"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elifesciences.org"],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>\n                    Parasites from the genus Plasmodium are the causative agents of malaria. The mobility, infectivity, and ultimately pathogenesis of\n                    <jats:italic>Plasmodium falciparum<\/jats:italic>\n                    rely on a macromolecular complex, called the glideosome. At the core of the glideosome is an essential and divergent Myosin A motor (PfMyoA), a first order drug target against malaria. Here, we present the full-length structure of PfMyoA in two states of its motor cycle. We report novel interactions that are essential for motor priming and the mode of recognition of its two light chains (PfELC and MTIP) by two degenerate IQ motifs. Kinetic and motility assays using PfMyoA variants, along with molecular dynamics, demonstrate how specific priming and atypical sequence adaptations tune the motor\u2019s mechano-chemical properties. Supported by evidence for an essential role of the PfELC in malaria pathogenesis, these structures provide a blueprint for the design of future anti-malarials targeting both the glideosome motor and its regulatory elements.\n                  <\/jats:p>","DOI":"10.7554\/elife.60581","type":"journal-article","created":{"date-parts":[[2020,10,12]],"date-time":"2020-10-12T20:00:16Z","timestamp":1602532816000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.60581","source":"Crossref","is-referenced-by-count":35,"title":["Full-length Plasmodium falciparum myosin A and essential light chain PfELC structures provide new anti-malarial targets"],"prefix":"10.7554","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1605-9619","authenticated-orcid":true,"given":"Dihia","family":"Moussaoui","sequence":"first","affiliation":[{"name":"Structural Motility, Institut Curie, Paris Universit\u00e9 Sciences et Lettres, Sorbonne Universit\u00e9, CNRS UMR144, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James P","family":"Robblee","sequence":"additional","affiliation":[{"name":"Department of Molecular Physiology and Biophysics, University of Vermont, Burlington, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Auguin","sequence":"additional","affiliation":[{"name":"Laboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), Universit\u00e9 d\u2019Orl\u00e9ans, INRAE, USC1328, Orl\u00e9ans, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena B","family":"Krementsova","sequence":"additional","affiliation":[{"name":"Department of Molecular Physiology and Biophysics, University of Vermont, Burlington, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Haase","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, Imperial College London, South Kensington, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8534-0025","authenticated-orcid":true,"given":"Thomas CA","family":"Blake","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, Imperial College London, South Kensington, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0275-352X","authenticated-orcid":true,"given":"Jake","family":"Baum","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, Imperial College London, South Kensington, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6102-2025","authenticated-orcid":true,"given":"Julien","family":"Robert-Paganin","sequence":"additional","affiliation":[{"name":"Structural Motility, Institut Curie, Paris Universit\u00e9 Sciences et Lettres, Sorbonne Universit\u00e9, CNRS UMR144, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5583-8500","authenticated-orcid":true,"given":"Kathleen M","family":"Trybus","sequence":"additional","affiliation":[{"name":"Department of Molecular Physiology and Biophysics, University of Vermont, Burlington, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8566-0336","authenticated-orcid":true,"given":"Anne","family":"Houdusse","sequence":"additional","affiliation":[{"name":"Structural Motility, Institut Curie, Paris Universit\u00e9 Sciences et Lettres, Sorbonne Universit\u00e9, CNRS UMR144, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4374","published-online":{"date-parts":[[2020,10,13]]},"reference":[{"key":"bib1","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1016\/j.softx.2015.06.001","article-title":"GROMACS: high performance molecular simulations through multi-level parallelism from laptops to supercomputers","volume":"1-2","author":"Abraham","year":"2015","journal-title":"SoftwareX"},{"key":"bib2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0032188","article-title":"Spatial localisation of actin filaments across developmental stages of the malaria parasite","volume":"7","author":"Angrisano","year":"2012","journal-title":"PLOS ONE"},{"key":"bib3","doi-asserted-by":"publisher","first-page":"5197","DOI":"10.1074\/jbc.M509807200","article-title":"A conserved molecular motor drives cell invasion and gliding motility across malaria life cycle stages and other apicomplexan parasites","volume":"281","author":"Baum","year":"2006","journal-title":"Journal of Biological Chemistry"},{"key":"bib4","doi-asserted-by":"publisher","first-page":"450","DOI":"10.1038\/nmeth.4223","article-title":"A genetic system to study Plasmodium falciparum protein function","volume":"14","author":"Birnbaum","year":"2017","journal-title":"Nature Methods"},{"key":"bib5","doi-asserted-by":"publisher","DOI":"10.1101\/2020.06.25.171900","article-title":"Actomyosin forces and the energetics of red blood cell invasion by the malaria parasite Plasmodium falciparum","volume-title":"bioRxiv","author":"Blake","year":"2020"},{"key":"bib6","doi-asserted-by":"publisher","first-page":"19290","DOI":"10.1074\/jbc.M117.813972","article-title":"Reconstitution of the core of the malaria parasite glideosome with recombinant Plasmodium class XIV myosin A and Plasmodium actin","volume":"292","author":"Bookwalter","year":"2017","journal-title":"Journal of Biological Chemistry"},{"key":"bib7","doi-asserted-by":"publisher","first-page":"4852","DOI":"10.1073\/pnas.0510907103","article-title":"Structure of the MTIP-MyoA complex, a key component of the malaria parasite invasion motor","volume":"103","author":"Bosch","year":"2006","journal-title":"PNAS"},{"key":"bib8","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1016\/j.jmb.2007.06.016","article-title":"The closed MTIP-myosin A-tail complex from the malaria parasite invasion machinery","volume":"372","author":"Bosch","year":"2007","journal-title":"Journal of Molecular Biology"},{"key":"bib9","doi-asserted-by":"publisher","first-page":"4559","DOI":"10.1182\/blood-2009-09-243725","article-title":"Interactions with heparin-like molecules during erythrocyte invasion by Plasmodium falciparum merozoites","volume":"115","author":"Boyle","year":"2010","journal-title":"Blood"},{"key":"bib10","article-title":"BUSTER","author":"Bricogne","year":"2017","unstructured":"Bricogne G. 2017. BUSTER. Glob Phasing Ltd. 2.10.2. https:\/\/www.globalphasing.com\/buster\/wiki\/index.cgi?BusterCite."},{"key":"bib11","doi-asserted-by":"publisher","first-page":"1545","DOI":"10.1002\/jcc.21287","article-title":"CHARMM: the biomolecular simulation program","volume":"30","author":"Brooks","year":"2009","journal-title":"Journal of Computational Chemistry"},{"key":"bib12","doi-asserted-by":"publisher","first-page":"30935","DOI":"10.1074\/jbc.M504698200","article-title":"Loop 1 of transducer region in mammalian class I myosin, Myo1b, modulates actin affinity, ATPase activity, and nucleotide access","volume":"280","author":"Clark","year":"2005","journal-title":"Journal of Biological Chemistry"},{"key":"bib13","doi-asserted-by":"publisher","first-page":"846","DOI":"10.1016\/j.bpj.2014.07.010","article-title":"Quantitation of malaria parasite-erythrocyte cell-cell interactions using optical tweezers","volume":"107","author":"Crick","year":"2014","journal-title":"Biophysical Journal"},{"key":"bib14","doi-asserted-by":"crossref","first-page":"10327","DOI":"10.1016\/S0021-9258(18)86949-6","article-title":"Biotination of proteins in vivo. A post-translational modification to label, purify, and study proteins","volume":"265","author":"Cronan","year":"1990","journal-title":"The Journal of Biological Chemistry"},{"key":"bib15","doi-asserted-by":"publisher","first-page":"690","DOI":"10.1038\/nmeth.2075","article-title":"Icy: an open bioimage informatics platform for extended reproducible research","volume":"9","author":"de Chaumont","year":"2012","journal-title":"Nature Methods"},{"key":"bib16","doi-asserted-by":"publisher","first-page":"36968","DOI":"10.1074\/jbc.M112.379842","article-title":"Regulation of the Plasmodium motor complex: phosphorylation of myosin A tail-interacting protein (MTIP) loosens its grip on MyoA","volume":"287","author":"Douse","year":"2012","journal-title":"The Journal of Biological Chemistry"},{"key":"bib17","doi-asserted-by":"publisher","first-page":"2126","DOI":"10.1107\/S0907444904019158","article-title":"Coot: model-building tools for molecular graphics","volume":"60","author":"Emsley","year":"2004","journal-title":"Acta Crystallographica. Section D, Biological Crystallography"},{"key":"bib18","doi-asserted-by":"publisher","first-page":"3681","DOI":"10.1073\/pnas.0506307103","article-title":"New insights into myosin evolution and classification","volume":"103","author":"Foth","year":"2006","journal-title":"PNAS"},{"key":"bib19","doi-asserted-by":"publisher","first-page":"1212","DOI":"10.1107\/S1600576717007786","article-title":"ATSAS 2.8: a comprehensive data analysis suite for small-angle scattering from macromolecular solutions","volume":"50","author":"Franke","year":"2017","journal-title":"Journal of Applied Crystallography"},{"key":"bib20","doi-asserted-by":"publisher","first-page":"645","DOI":"10.1038\/nrmicro.2017.86","article-title":"Gliding motility powers invasion and egress in apicomplexa","volume":"15","author":"Fr\u00e9nal","year":"2017","journal-title":"Nature Reviews Microbiology"},{"key":"bib21","doi-asserted-by":"publisher","first-page":"17857","DOI":"10.1074\/jbc.M117.802769","article-title":"Compositional and expression analyses of the glideosome during the plasmodium life cycle reveal an additional myosin light chain required for maximum motility","volume":"292","author":"Green","year":"2017","journal-title":"Journal of Biological Chemistry"},{"key":"bib22","doi-asserted-by":"publisher","first-page":"E3337","DOI":"10.1073\/pnas.1506633112","article-title":"Mechanochemical tuning of myosin-I by the N-terminal region","volume":"112","author":"Greenberg","year":"2015","journal-title":"PNAS"},{"key":"bib23","doi-asserted-by":"publisher","first-page":"156","DOI":"10.1038\/nrmicro.2017.161","article-title":"Drug resistance in Plasmodium","volume":"16","author":"Haldar","year":"2018","journal-title":"Nature Reviews Microbiology"},{"key":"bib24","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-018-3278-6","article-title":"Distribution of Anopheles vectors and potential malaria transmission stability in Europe and the Mediterranean area under future climate change","volume":"12","author":"Hertig","year":"2019","journal-title":"Parasites & Vectors"},{"key":"bib25","doi-asserted-by":"publisher","first-page":"11238","DOI":"10.1073\/pnas.200376897","article-title":"Three conformational states of scallop myosin S1","volume":"97","author":"Houdusse","year":"2000","journal-title":"PNAS"},{"key":"bib26","doi-asserted-by":"publisher","first-page":"10644","DOI":"10.1073\/pnas.92.23.10644","article-title":"Target sequence recognition by the calmodulin superfamily: implications from light chain binding to the regulatory domain of scallop myosin","volume":"92","author":"Houdusse","year":"1995","journal-title":"PNAS"},{"key":"bib27","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1016\/S0969-2126(96)00006-8","article-title":"Structure of the regulatory domain of scallop myosin at 2 A resolution: implications for regulation","volume":"4","author":"Houdusse","year":"1996","journal-title":"Structure"},{"key":"bib28","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1038\/nmeth.4067","article-title":"CHARMM36m: an improved force field for folded and intrinsically disordered proteins","volume":"14","author":"Huang","year":"2017","journal-title":"Nature Methods"},{"key":"bib29","doi-asserted-by":"publisher","first-page":"1114","DOI":"10.1002\/jcc.24660","article-title":"CHARMM-GUI 10 years for biomolecular modeling and simulation","volume":"38","author":"Jo","year":"2017","journal-title":"Journal of Computational Chemistry"},{"key":"bib30","doi-asserted-by":"publisher","first-page":"246","DOI":"10.1016\/j.chom.2012.06.005","article-title":"Analysis of protein palmitoylation reveals a pervasive role in Plasmodium\u00a0development and pathogenesis","volume":"12","author":"Jones","year":"2012","journal-title":"Cell Host & Microbe"},{"key":"bib31","doi-asserted-by":"publisher","DOI":"10.1038\/srep21800","article-title":"A versatile strategy for rapid conditional genome engineering using loxP sites in a small synthetic intron in Plasmodium falciparum","volume":"6","author":"Jones","year":"2016","journal-title":"Scientific Reports"},{"key":"bib32","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1107\/S0907444909047337","article-title":"XDS","volume":"66","author":"Kabsch","year":"2010","journal-title":"Acta Crystallographica. Section D, Biological Crystallography"},{"key":"bib33","doi-asserted-by":"publisher","first-page":"1623","DOI":"10.1529\/biophysj.106.097618","article-title":"Mutation of a conserved glycine in the SH1-SH2 Helix affects the load-dependent kinetics of myosin","volume":"92","author":"Kad","year":"2007","journal-title":"Biophysical Journal"},{"key":"bib34","doi-asserted-by":"publisher","first-page":"895","DOI":"10.1083\/jcb.134.4.895","article-title":"Glycine 699 is pivotal for the motor activity of skeletal muscle myosin","volume":"134","author":"Kinose","year":"1996","journal-title":"The Journal of Cell Biology"},{"key":"bib35","doi-asserted-by":"publisher","first-page":"658","DOI":"10.1107\/S0021889807021206","article-title":"Phaser crystallographic software","volume":"40","author":"McCoy","year":"2007","journal-title":"Journal of Applied Crystallography"},{"key":"bib36","doi-asserted-by":"publisher","first-page":"1292","DOI":"10.1073\/pnas.1718316115","article-title":"High-resolution cryo-EM structures of actin-bound myosin states reveal the mechanism of myosin force sensing","volume":"115","author":"Mentes","year":"2018","journal-title":"PNAS"},{"key":"bib37","doi-asserted-by":"publisher","first-page":"2070","DOI":"10.1016\/j.jmb.2014.03.004","article-title":"Crystal structure of human myosin 1c--the motor in GLUT4 exocytosis: implications for Ca2+ regulation and 14-3-3 binding","volume":"426","author":"M\u00fcnnich","year":"2014","journal-title":"Journal of Molecular Biology"},{"key":"bib38","doi-asserted-by":"publisher","first-page":"551","DOI":"10.1016\/j.chom.2009.11.007","article-title":"Plasmodium sporozoite motility is modulated by the turnover of discrete adhesion sites","volume":"6","author":"M\u00fcnter","year":"2009","journal-title":"Cell Host & Microbe"},{"key":"bib39","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(82)85020-9","article-title":"Purification of muscle actin","volume":"85","author":"Pardee","year":"1982","journal-title":"Methods in Enzymology"},{"key":"bib40","doi-asserted-by":"publisher","DOI":"10.1101\/867499","article-title":"Structural role of essential light chains in the apicomplexan glideosome","volume-title":"bioRxiv","author":"Pazicky","year":"2019"},{"key":"bib41","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00905-18","article-title":"The actinomyosin motor drives malaria parasite red blood cell invasion but not egress","volume":"9","author":"Perrin","year":"2018","journal-title":"mBio"},{"key":"bib42","doi-asserted-by":"publisher","first-page":"E10548","DOI":"10.1073\/pnas.1811167115","article-title":"Structural and mechanistic insights into the function of the unconventional class XIV myosin MyoA from Toxoplasma gondii","volume":"115","author":"Powell","year":"2018","journal-title":"PNAS"},{"key":"bib43","doi-asserted-by":"publisher","first-page":"35051","DOI":"10.1074\/jbc.M111.265298","article-title":"Structural basis for the allosteric interference of myosin function by reactive thiol region mutations G680A and G680V","volume":"286","author":"Preller","year":"2011","journal-title":"Journal of Biological Chemistry"},{"key":"bib44","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1073\/pnas.1017676108","article-title":"Essential \u201cankle\u201d in the myosin lever arm: Fig. 1","volume":"108","author":"Pylypenko","year":"2011","journal-title":"PNAS"},{"key":"bib45","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-06191-4","article-title":"Hypertrophic cardiomyopathy disease results from disparate impairments of cardiac myosin function and auto-inhibition","volume":"9","author":"Robert-Paganin","year":"2018","journal-title":"Nature Communications"},{"key":"bib46","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-11120-0","article-title":"Plasmodium myosin A drives parasite invasion by an atypical force generating mechanism","volume":"10","author":"Robert-Paganin","year":"2019","journal-title":"Nature Communications"},{"key":"bib47","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1021\/acs.chemrev.9b00264","article-title":"Force generation by myosin motors: a structural perspective","volume":"120","author":"Robert-Paganin","year":"2020","journal-title":"Chemical Reviews"},{"key":"bib48","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms12456","article-title":"The myosin X motor is optimized for movement on actin bundles","volume":"7","author":"Ropars","year":"2016","journal-title":"Nature Communications"},{"key":"bib49","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-020-03224-6","article-title":"Shifting transmission risk for malaria in Africa with climate change: a framework for planning and intervention","volume":"19","author":"Ryan","year":"2020","journal-title":"Malaria Journal"},{"key":"bib50","doi-asserted-by":"publisher","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: an open-source platform for biological-image analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nature Methods"},{"key":"bib51","doi-asserted-by":"publisher","first-page":"W424","DOI":"10.1093\/nar\/gkw389","article-title":"FoXS, FoXSDock and MultiFoXS: single-state and multi-state structural modeling of proteins and their complexes based on SAXS profiles","volume":"44","author":"Schneidman-Duhovny","year":"2016","journal-title":"Nucleic Acids Research"},{"key":"bib52","doi-asserted-by":"publisher","first-page":"2116","DOI":"10.1073\/pnas.1321022111","article-title":"A vertebrate myosin-I structure reveals unique insights into myosin mechanochemical tuning","volume":"111","author":"Shuman","year":"2014","journal-title":"PNAS"},{"key":"bib53","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01170-19","article-title":"A novel tool for the generation of conditional knockouts to study gene function across the Plasmodium falciparum life cycle","volume":"10","author":"Tib\u00farcio","year":"2019","journal-title":"mBio"},{"key":"bib54","doi-asserted-by":"publisher","first-page":"673","DOI":"10.1126\/science.781840","article-title":"Human malaria parasites in continuous culture","volume":"193","author":"Trager","year":"1976","journal-title":"Science"},{"key":"bib55","doi-asserted-by":"publisher","first-page":"12403","DOI":"10.1073\/pnas.91.26.12403","article-title":"The essential light chain is required for full force production by skeletal muscle myosin","volume":"91","author":"VanBuren","year":"1994","journal-title":"PNAS"},{"key":"bib56","doi-asserted-by":"publisher","first-page":"293","DOI":"10.1107\/S0907444911007773","article-title":"Data processing and analysis with the autoPROC toolbox","volume":"67","author":"Vonrhein","year":"2011","journal-title":"Acta Crystallographica. Section D, Biological Crystallography"},{"key":"bib57","doi-asserted-by":"publisher","first-page":"W296","DOI":"10.1093\/nar\/gky427","article-title":"SWISS-MODEL: homology modelling of protein structures and complexes","volume":"46","author":"Waterhouse","year":"2018","journal-title":"Nucleic Acids Research"},{"key":"bib58","volume-title":"World Malaria Report 2018","author":"WHO","year":"2018","unstructured":"WHO. 2018. World Health Organization. World Malaria Report 2018."},{"key":"bib59","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00845-15","article-title":"Two essential light chains regulate the MyoA lever arm to promote Toxoplasma gliding motility","volume":"6","author":"Williams","year":"2015","journal-title":"mBio"}],"container-title":["eLife"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/cdn.elifesciences.org\/articles\/60581\/elife-60581-v1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/cdn.elifesciences.org\/articles\/60581\/elife-60581-v1.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/elifesciences.org\/articles\/60581","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T21:15:34Z","timestamp":1697058934000},"score":1,"resource":{"primary":{"URL":"https:\/\/elifesciences.org\/articles\/60581"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10,13]]},"references-count":59,"alternative-id":["10.7554\/eLife.60581"],"URL":"https:\/\/doi.org\/10.7554\/elife.60581","archive":["CLOCKSS"],"relation":{"is-supplemented-by":[{"id-type":"accession","id":"6YCY","asserted-by":"subject"},{"id-type":"accession","id":"6YCZ","asserted-by":"subject"},{"id-type":"accession","id":"6YCX","asserted-by":"subject"}],"has-review":[{"id-type":"doi","id":"10.7554\/eLife.60581.sa2","asserted-by":"object"},{"id-type":"doi","id":"10.7554\/eLife.60581.sa1","asserted-by":"object"}]},"ISSN":["2050-084X"],"issn-type":[{"value":"2050-084X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,10,13]]},"article-number":"e60581"}}