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However, insights into organ architecture are currently hampered by an absence of standardized quantitative 3D analysis. We aimed to develop a robust technology to visualize, digitalize, and segment the architecture of two tubular systems in 3D:\n                    <jats:italic>d<\/jats:italic>\n                    o\n                    <jats:italic>u<\/jats:italic>\n                    ble resin\n                    <jats:italic>c<\/jats:italic>\n                    asting micro computed\n                    <jats:italic>t<\/jats:italic>\n                    omography (DUCT). As proof of principle, we applied DUCT to a mouse model for Alagille syndrome (\n                    <jats:italic>\n                      Jag1\n                      <jats:sup>Ndr\/Ndr<\/jats:sup>\n                    <\/jats:italic>\n                    mice), characterized by intrahepatic bile duct paucity, that can spontaneously generate a biliary system in adulthood. DUCT identified increased central biliary branching and peripheral bile duct tortuosity as two compensatory processes occurring in distinct regions of\n                    <jats:italic>\n                      Jag1\n                      <jats:sup>Ndr\/Ndr<\/jats:sup>\n                    <\/jats:italic>\n                    liver, leading to full reconstitution of wild-type biliary volume and phenotypic recovery. DUCT is thus a powerful new technology for 3D analysis, which can reveal novel phenotypes and provide a standardized method of defining liver architecture in mouse models.\n                  <\/jats:p>","DOI":"10.7554\/elife.60916","type":"journal-article","created":{"date-parts":[[2021,2,26]],"date-time":"2021-02-26T09:00:21Z","timestamp":1614330021000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.60916","source":"Crossref","is-referenced-by-count":20,"title":["DUCT reveals architectural mechanisms contributing to bile duct recovery in a mouse model for Alagille syndrome"],"prefix":"10.7554","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7797-3818","authenticated-orcid":true,"given":"Simona","family":"Hankeova","sequence":"first","affiliation":[{"name":"Department of Biosciences and Nutrition, Karolinska Institutet, Solna, Sweden"},{"name":"Department of Experimental Biology, Masaryk University, Brno, Czech 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