{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T05:27:30Z","timestamp":1790400450511,"version":"4.1.0"},"reference-count":39,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2020,11,18]],"date-time":"2020-11-18T00:00:00Z","timestamp":1605657600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,11,18]],"date-time":"2020-11-18T00:00:00Z","timestamp":1605657600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,11,18]],"date-time":"2020-11-18T00:00:00Z","timestamp":1605657600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002855","name":"Ministry of Science and Technology of the People's Republic of China","doi-asserted-by":"publisher","award":["Institutional"],"award-info":[{"award-number":["Institutional"]}],"id":[{"id":"10.13039\/501100002855","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002888","name":"Beijing Municipal Commission of Education","doi-asserted-by":"publisher","award":["Institutional"],"award-info":[{"award-number":["Institutional"]}],"id":[{"id":"10.13039\/501100002888","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elifesciences.org"],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>\n                    Casein kinases are a large family of intracellular serine\/threonine kinases that control a variety of cellular signaling functions. Here we report that a member of casein kinase 1 family, casein kinase 1G2, CSNK1G2, binds and inhibits the activation of receptor-interacting kinase 3, RIPK3, thereby attenuating RIPK3-mediated necroptosis. The binding of CSNK1G2 to RIPK3 is triggered by auto-phosphorylation at serine 211\/threonine 215 sites in its C-terminal domain. CSNK1G2-knockout mice showed significantly enhanced necroptosis response and premature aging of their testis, a phenotype that was rescued by either double knockout of the\n                    <jats:italic>Ripk3<\/jats:italic>\n                    gene or feeding the animal with a RIPK1 kinase inhibitor-containing diet. Moreover, CSNK1G2 is also co-expressed with RIPK3 in human testis, and the necroptosis activation marker phospho-MLKL was observed in the testis of old (&gt;80) but not young men, indicating that the testis-aging program carried out by the RIPK3-mediated and CSNK1G2-attenuated necroptosis is evolutionarily conserved between mice and men.\n                  <\/jats:p>","DOI":"10.7554\/elife.61564","type":"journal-article","created":{"date-parts":[[2020,11,18]],"date-time":"2020-11-18T11:00:39Z","timestamp":1605697239000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.61564","source":"Crossref","is-referenced-by-count":29,"title":["Casein kinase 1G2 suppresses necroptosis-promoted testis aging by inhibiting receptor-interacting kinase 3"],"prefix":"10.7554","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5564-3033","authenticated-orcid":true,"given":"Dianrong","family":"Li","sequence":"first","affiliation":[{"name":"National Institute of Biological Sciences, Beijing, China"},{"name":"Tsinghua Institute of Multidisciplinary Biomedical Research, Tsinghua University, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youwei","family":"Ai","sequence":"additional","affiliation":[{"name":"National Institute of Biological Sciences, Beijing, China"},{"name":"Tsinghua Institute of Multidisciplinary Biomedical Research, Tsinghua University, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Guo","sequence":"additional","affiliation":[{"name":"National Institute of Biological Sciences, Beijing, China"},{"name":"Tsinghua Institute of Multidisciplinary Biomedical Research, Tsinghua University, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baijun","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of Urology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Xu","sequence":"additional","affiliation":[{"name":"National Institute of Biological Sciences, Beijing, China"},{"name":"Tsinghua Institute of Multidisciplinary Biomedical Research, Tsinghua University, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengchao","family":"Wang","sequence":"additional","affiliation":[{"name":"National Institute of Biological Sciences, Beijing, China"},{"name":"Tsinghua Institute of Multidisciplinary Biomedical Research, Tsinghua University, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9885-356X","authenticated-orcid":true,"given":"Xiaodong","family":"Wang","sequence":"additional","affiliation":[{"name":"National Institute of Biological Sciences, Beijing, China"},{"name":"Tsinghua Institute of Multidisciplinary Biomedical Research, Tsinghua University, Beijing, 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