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Here, we present structures of two such accessory factors from the oncogenic herpesviruses Kaposi\u2019s sarcoma-associated herpesvirus (KSHV; ORF68) and Epstein\u2013Barr virus (EBV; BFLF1). These homologous proteins form highly similar homopentameric rings with a positively charged central channel that binds double-stranded DNA. Mutation of individual positively charged residues within but not outside the channel ablates DNA binding, and in the context of KSHV infection, these mutants fail to package the viral genome or produce progeny virions. Thus, we propose a model in which ORF68 facilitates the transfer of newly replicated viral genomes to the packaging motor.<\/jats:p>","DOI":"10.7554\/elife.62261","type":"journal-article","created":{"date-parts":[[2021,2,8]],"date-time":"2021-02-08T08:01:09Z","timestamp":1612771269000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.62261","source":"Crossref","is-referenced-by-count":18,"title":["A pentameric protein ring with novel architecture is required for herpesviral packaging"],"prefix":"10.7554","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7277-5233","authenticated-orcid":true,"given":"Allison L","family":"Didychuk","sequence":"first","affiliation":[{"name":"Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4312-2900","authenticated-orcid":true,"given":"Stephanie 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