{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T22:29:16Z","timestamp":1789770556943,"version":"4.0.1"},"reference-count":59,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2021,1,22]],"date-time":"2021-01-22T00:00:00Z","timestamp":1611273600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,1,22]],"date-time":"2021-01-22T00:00:00Z","timestamp":1611273600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,1,22]],"date-time":"2021-01-22T00:00:00Z","timestamp":1611273600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","award":["R01 DE012711"],"award-info":[{"award-number":["R01 DE012711"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","award":["U01 DE028729"],"award-info":[{"award-number":["U01 DE028729"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elifesciences.org"],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>\n                    Cranial neural crest (CNC) cells give rise to bone, cartilage, tendons, and ligaments of the vertebrate craniofacial musculoskeletal complex, as well as regulate mesoderm-derived craniofacial muscle development through cell-cell interactions. Using the mouse soft palate as a model, we performed an unbiased single-cell RNA-seq analysis to investigate the heterogeneity and lineage commitment of CNC derivatives during craniofacial muscle development. We show that Runx2, a known osteogenic regulator, is expressed in the CNC-derived perimysial and progenitor populations. Loss of\n                    <jats:italic>Runx2<\/jats:italic>\n                    in CNC-derivatives results in reduced expression of perimysial markers (\n                    <jats:italic>Aldh1a2<\/jats:italic>\n                    and\n                    <jats:italic>Hic1<\/jats:italic>\n                    ) as well as soft palate muscle defects in\n                    <jats:italic>\n                      Osr2-Cre;Runx2\n                      <jats:sup>fl\/fl<\/jats:sup>\n                    <\/jats:italic>\n                    mice. We further reveal that Runx2 maintains perimysial marker expression through suppressing\n                    <jats:italic>Twist1,<\/jats:italic>\n                    and that myogenesis is restored in\n                    <jats:italic>\n                      Osr2-Cre;Runx2\n                      <jats:sup>fl\/fl<\/jats:sup>\n                      ;Twist1\n                      <jats:sup>fl\/+<\/jats:sup>\n                    <\/jats:italic>\n                    mice. Collectively, our findings highlight the roles of Runx2, Twist1, and their interaction in regulating the fate of CNC-derived cells as they guide craniofacial muscle development through cell-cell interactions.\n                  <\/jats:p>","DOI":"10.7554\/elife.62387","type":"journal-article","created":{"date-parts":[[2021,1,22]],"date-time":"2021-01-22T13:10:16Z","timestamp":1611321016000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.62387","source":"Crossref","is-referenced-by-count":40,"title":["Runx2-Twist1 interaction coordinates cranial neural crest guidance of soft palate myogenesis"],"prefix":"10.7554","volume":"10","author":[{"given":"Xia","family":"Han","sequence":"first","affiliation":[{"name":"Center for Craniofacial Molecular Biology, University of Southern California, Los Angeles, Los Angeles, United 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