{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T06:13:12Z","timestamp":1777443192272,"version":"3.51.4"},"reference-count":80,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2021,6,9]],"date-time":"2021-06-09T00:00:00Z","timestamp":1623196800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,6,9]],"date-time":"2021-06-09T00:00:00Z","timestamp":1623196800000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,6,9]],"date-time":"2021-06-09T00:00:00Z","timestamp":1623196800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["AI154931"],"award-info":[{"award-number":["AI154931"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["AI128356"],"award-info":[{"award-number":["AI128356"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["T32AI060546"],"award-info":[{"award-number":["T32AI060546"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["DK101585"],"award-info":[{"award-number":["DK101585"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elifesciences.org"],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>\n                    Transient receptor potential (TRP) channels participate in calcium ion (Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ) influx and intracellular Ca\n                    <jats:sup>2+<\/jats:sup>\n                    release. TRP channels have not been studied in\n                    <jats:italic>Toxoplasma gondii<\/jats:italic>\n                    or any other apicomplexan parasite. In this work, we characterize TgGT1_310560, a protein predicted to possess a TRP domain (TgTRPPL-2), and determined its role in Ca\n                    <jats:sup>2+<\/jats:sup>\n                    signaling in\n                    <jats:italic>T. gondii<\/jats:italic>\n                    , the causative agent of toxoplasmosis. TgTRPPL-2 localizes to the plasma membrane and the endoplasmic reticulum (ER) of\n                    <jats:italic>T. gondii<\/jats:italic>\n                    . The\n                    <jats:italic>\u0394TgTRPPL-2<\/jats:italic>\n                    mutant was defective in growth and cytosolic Ca\n                    <jats:sup>2+<\/jats:sup>\n                    influx from both extracellular and intracellular sources. Heterologous expression of TgTRPPL-2 in HEK-3KO cells allowed its functional characterization. Patching of ER-nuclear membranes demonstrates that TgTRPPL-2 is a non-selective cation channel that conducts Ca\n                    <jats:sup>2+<\/jats:sup>\n                    . Pharmacological blockers of TgTRPPL-2 inhibit Ca\n                    <jats:sup>2+<\/jats:sup>\n                    influx and parasite growth. This is the first report of an apicomplexan ion channel that conducts Ca\n                    <jats:sup>2+<\/jats:sup>\n                    and may initiate a Ca\n                    <jats:sup>2+<\/jats:sup>\n                    signaling cascade that leads to the stimulation of motility, invasion, and egress. TgTRPPL-2 is a potential target for combating toxoplasmosis.\n                  <\/jats:p>","DOI":"10.7554\/elife.63417","type":"journal-article","created":{"date-parts":[[2021,6,9]],"date-time":"2021-06-09T08:00:41Z","timestamp":1623225641000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.63417","source":"Crossref","is-referenced-by-count":29,"title":["Calcium signaling through a transient receptor channel is important for Toxoplasma gondii growth"],"prefix":"10.7554","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9459-6882","authenticated-orcid":true,"given":"Karla Marie","family":"M\u00e1rquez-Nogueras","sequence":"first","affiliation":[{"name":"Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, United States"},{"name":"Department of Cell and Molecular Physiology, Stritch School of Medicine, Loyola University Chicago, Maywood, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miryam Andrea","family":"Hortua Triana","sequence":"additional","affiliation":[{"name":"Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathan M","family":"Chasen","sequence":"additional","affiliation":[{"name":"Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9867-2408","authenticated-orcid":true,"given":"Ivana Y","family":"Kuo","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Physiology, Stritch School of Medicine, Loyola University Chicago, Maywood, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2041-6295","authenticated-orcid":true,"given":"Silvia NJ","family":"Moreno","sequence":"additional","affiliation":[{"name":"Center for Tropical and 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