{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T13:08:14Z","timestamp":1784898494810,"version":"3.55.0"},"reference-count":64,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2021,2,23]],"date-time":"2021-02-23T00:00:00Z","timestamp":1614038400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,2,23]],"date-time":"2021-02-23T00:00:00Z","timestamp":1614038400000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,2,23]],"date-time":"2021-02-23T00:00:00Z","timestamp":1614038400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["GM128168"],"award-info":[{"award-number":["GM128168"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HD080224"],"award-info":[{"award-number":["HD080224"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HD038082"],"award-info":[{"award-number":["HD038082"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["DP1ES025458"],"award-info":[{"award-number":["DP1ES025458"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007919","name":"University of Massachusetts","doi-asserted-by":"publisher","award":["DK32520"],"award-info":[{"award-number":["DK32520"]}],"id":[{"id":"10.13039\/100007919","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elifesciences.org"],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>\n                    The X-linked gene\n                    <jats:italic>Rlim<\/jats:italic>\n                    plays major roles in female mouse development and reproduction, where it is crucial for the maintenance of imprinted X chromosome inactivation in extraembryonic tissues of embryos. However, while females carrying a systemic\n                    <jats:italic>Rlim<\/jats:italic>\n                    knockout (KO) die around implantation, male\n                    <jats:italic>Rlim<\/jats:italic>\n                    KO mice appear healthy and are fertile. Here, we report an important role for\n                    <jats:italic>Rlim<\/jats:italic>\n                    in testis where it is highly expressed in post-meiotic round spermatids as well as in Sertoli cells. Systemic deletion of the\n                    <jats:italic>Rlim<\/jats:italic>\n                    gene results in lower numbers of mature sperm that contains excess cytoplasm, leading to decreased sperm motility and in vitro fertilization rates. Targeting the conditional\n                    <jats:italic>Rlim<\/jats:italic>\n                    cKO specifically to the spermatogenic cell lineage largely recapitulates this phenotype. These results reveal functions of\n                    <jats:italic>Rlim<\/jats:italic>\n                    in male reproduction specifically in round spermatids during spermiogenesis.\n                  <\/jats:p>","DOI":"10.7554\/elife.63556","type":"journal-article","created":{"date-parts":[[2021,2,23]],"date-time":"2021-02-23T08:00:35Z","timestamp":1614067235000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.63556","source":"Crossref","is-referenced-by-count":15,"title":["Deficient spermiogenesis in mice lacking Rlim"],"prefix":"10.7554","volume":"10","author":[{"given":"Feng","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School, Worcester, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5468-2700","authenticated-orcid":true,"given":"Maria 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Massachusetts Amherst, Amherst, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lihua Julie","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School, Worcester, United States"},{"name":"Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, United States"},{"name":"Program in Bioinformatics and Integrative Biology, University of Massachusetts Medical School, Worcester, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1516-9397","authenticated-orcid":true,"given":"Oliver J","family":"Rando","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pablo 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