{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T15:24:26Z","timestamp":1787239466149,"version":"build-2736575974"},"reference-count":88,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2021,7,7]],"date-time":"2021-07-07T00:00:00Z","timestamp":1625616000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,7,7]],"date-time":"2021-07-07T00:00:00Z","timestamp":1625616000000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,7,7]],"date-time":"2021-07-07T00:00:00Z","timestamp":1625616000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100004440","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["102908\/Z\/13\/Z"],"award-info":[{"award-number":["102908\/Z\/13\/Z"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100011725","name":"Meningitis Now","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100011725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elifesciences.org"],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>\n                    Type VI Secretion Systems (T6SSs) are widespread in bacteria and can dictate the development and organisation of polymicrobial ecosystems by mediating contact dependent killing. In\n                    <jats:italic>Neisseria<\/jats:italic>\n                    species, including\n                    <jats:italic>Neisseria cinerea<\/jats:italic>\n                    a commensal of the human respiratory tract, interbacterial contacts are mediated by Type four pili (Tfp) which promote formation of aggregates and govern the spatial dynamics of growing\n                    <jats:italic>Neisseria<\/jats:italic>\n                    microcolonies. Here, we show that\n                    <jats:italic>N. cinerea<\/jats:italic>\n                    expresses a plasmid-encoded T6SS that is active and can limit growth of related pathogens. We explored the impact of Tfp on\n                    <jats:italic>N. cinerea<\/jats:italic>\n                    T6SS-dependent killing within a colony and show that pilus expression by a prey strain enhances susceptibility to T6SS compared to a non-piliated prey, by preventing segregation from a T6SS-wielding attacker. Our findings have important implications for understanding how spatial constraints during contact-dependent antagonism can shape the evolution of microbial communities.\n                  <\/jats:p>","DOI":"10.7554\/elife.63755","type":"journal-article","created":{"date-parts":[[2021,7,7]],"date-time":"2021-07-07T05:00:28Z","timestamp":1625634028000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.63755","source":"Crossref","is-referenced-by-count":34,"title":["Type VI secretion system killing by commensal Neisseria is influenced by expression of type four pili"],"prefix":"10.7554","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7561-5515","authenticated-orcid":true,"given":"Rafael","family":"Custodio","sequence":"first","affiliation":[{"name":"Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rhian M","family":"Ford","sequence":"additional","affiliation":[{"name":"Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cara J","family":"Ellison","sequence":"additional","affiliation":[{"name":"Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangyu","family":"Liu","sequence":"additional","affiliation":[{"name":"Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2477-7565","authenticated-orcid":true,"given":"Gerda","family":"Mickute","sequence":"additional","affiliation":[{"name":"Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8366-3245","authenticated-orcid":true,"given":"Christoph M","family":"Tang","sequence":"additional","affiliation":[{"name":"Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9120-5586","authenticated-orcid":true,"given":"Rachel M","family":"Exley","sequence":"additional","affiliation":[{"name":"Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4374","published-online":{"date-parts":[[2021,7,7]]},"reference":[{"key":"bib1","doi-asserted-by":"publisher","first-page":"1545","DOI":"10.1038\/s41564-019-0479-5","article-title":"DNA-uptake pili of Vibrio cholerae are required for chitin colonization and capable of kin recognition via sequence-specific self-interaction","volume":"4","author":"Adams","year":"2019","journal-title":"Nature 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