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However, how these chondrocytes are maintained in the resting zone remains undefined. We undertook a genetic pulse-chase approach to isolate slow cycling, label-retaining chondrocytes (LRCs) using a chondrocyte-specific doxycycline-controllable Tet-Off system regulating expression of histone 2B-linked GFP. Comparative RNA-seq analysis identified significant enrichment of inhibitors and activators for Wnt signaling in LRCs and non-LRCs, respectively. Activation of Wnt\/\u03b2-catenin signaling in PTHrP\n                    <jats:sup>+<\/jats:sup>\n                    resting chondrocytes using\n                    <jats:italic>Pthlh-creER<\/jats:italic>\n                    and\n                    <jats:italic>Apc<\/jats:italic>\n                    -floxed allele impaired their ability to form columnar chondrocytes. Therefore, slow-cycling chondrocytes are maintained in a Wnt-inhibitory environment within the resting zone, unraveling a novel mechanism regulating maintenance and differentiation of PTHrP\n                    <jats:sup>+<\/jats:sup>\n                    skeletal stem cells of the postnatal growth plate.\n                  <\/jats:p>","DOI":"10.7554\/elife.64513","type":"journal-article","created":{"date-parts":[[2021,7,26]],"date-time":"2021-07-26T06:00:26Z","timestamp":1627279226000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.64513","source":"Crossref","is-referenced-by-count":68,"title":["Chondrocytes in the resting zone of the growth plate are maintained in a Wnt-inhibitory environment"],"prefix":"10.7554","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1472-7502","authenticated-orcid":true,"given":"Shawn A","family":"Hallett","sequence":"first","affiliation":[{"name":"University of Michigan School of Dentistry, Ann Arbor, United 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