{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T01:20:35Z","timestamp":1776475235102,"version":"3.51.2"},"reference-count":34,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2021,11,3]],"date-time":"2021-11-03T00:00:00Z","timestamp":1635897600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"},{"start":{"date-parts":[[2021,11,3]],"date-time":"2021-11-03T00:00:00Z","timestamp":1635897600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"},{"start":{"date-parts":[[2021,11,3]],"date-time":"2021-11-03T00:00:00Z","timestamp":1635897600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01-AI078881"],"award-info":[{"award-number":["R01-AI078881"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01-AI116868"],"award-info":[{"award-number":["R01-AI116868"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["PI15\/00151"],"award-info":[{"award-number":["PI15\/00151"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Secretaria d'Universitats i Recerca del Departament d'Economia i Coneixement","award":["grant 2017_SGR_1753"],"award-info":[{"award-number":["grant 2017_SGR_1753"]}]},{"name":"CERCA Programme\/Generalitat de Catalunya"},{"DOI":"10.13039\/501100001655","name":"Germany Academic Exchange Service","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001655","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R01GM121600"],"award-info":[{"award-number":["R01GM121600"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["PI13\/00155"],"award-info":[{"award-number":["PI13\/00155"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01-OD011095"],"award-info":[{"award-number":["R01-OD011095"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elifesciences.org"],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2021,11,1]]},"abstract":"<jats:p>\n                    While the liver, specifically hepatocytes, are widely accepted as the main source of hepatitis C virus (HCV) production, the role of the liver\/hepatocytes in clearance of circulating HCV remains unknown. Frequent HCV kinetic data were recorded and mathematically modeled from five liver transplant patients throughout the anhepatic (absence of liver) phase and for 4 hr post-reperfusion. During the anhepatic phase, HCV remained at pre-anhepatic levels (\n                    <jats:italic>n<\/jats:italic>\n                    = 3) or declined (\n                    <jats:italic>n<\/jats:italic>\n                    = 2) with\n                    <jats:italic>t<\/jats:italic>\n                    <jats:sub>1\/2<\/jats:sub>\n                    ~1 hr. Immediately post-reperfusion, virus declined in a biphasic manner in four patients consisting of a rapid decline (\n                    <jats:italic>t<\/jats:italic>\n                    <jats:sub>1\/2<\/jats:sub>\n                    = 5 min) followed by a slower decline (\n                    <jats:italic>t<\/jats:italic>\n                    <jats:sub>1\/2<\/jats:sub>\n                    = 67 min). Consistent with the majority of patients in the anhepatic phase, when we monitored HCV clearance at 37\u00b0C from culture medium in the absence\/presence of chronically infected hepatoma cells that were inhibited from secreting HCV, the HCV\n                    <jats:italic>t<\/jats:italic>\n                    <jats:sub>1\/2<\/jats:sub>\n                    in cell culture was longer in the absence of chronically HCV-infected cells. The results suggest that the liver plays a major role in the clearance of circulating HCV and that hepatocytes may be involved.\n                  <\/jats:p>","DOI":"10.7554\/elife.65297","type":"journal-article","created":{"date-parts":[[2021,11,3]],"date-time":"2021-11-03T09:00:28Z","timestamp":1635930028000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.65297","source":"Crossref","is-referenced-by-count":5,"title":["Modeling hepatitis C virus kinetics during liver transplantation reveals the role of the liver in virus clearance"],"prefix":"10.7554","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5273-8363","authenticated-orcid":true,"given":"Louis","family":"Shekhtman","sequence":"first","affiliation":[{"name":"The Program for Experimental & Theoretical Modeling, Division of Hepatology, Department of Medicine, Stritch School of Medicine, Loyola University Medical Center","place":["Maywood, IL, United States"]},{"name":"Network Science Institute, Northeastern University","place":["Boston, MA, United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3130-9604","authenticated-orcid":true,"given":"Miquel","family":"Navasa","sequence":"first","affiliation":[{"name":"Liver Unit, Hospital Cl\u00ednic, IDIBAPS and CIBEREHD, University of Barcelona","place":["Barcelona, Spain"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natasha","family":"Sansone","sequence":"first","affiliation":[{"name":"The Program for Experimental & Theoretical Modeling, Division of Hepatology, Department of Medicine, Stritch School of Medicine, Loyola University Medical Center","place":["Maywood, IL, United States"]},{"name":"Department of Microbiology & Immunology, University of Illinois Chicago","place":["Chicago, IL, United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gonzalo","family":"Crespo","sequence":"additional","affiliation":[{"name":"Liver Unit, Hospital Cl\u00ednic, IDIBAPS and CIBEREHD, University of Barcelona","place":["Barcelona, Spain"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gitanjali","family":"Subramanya","sequence":"additional","affiliation":[{"name":"The Program for Experimental & Theoretical Modeling, Division of Hepatology, Department of Medicine, Stritch School of Medicine, Loyola University Medical Center","place":["Maywood, IL, United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tje Lin","family":"Chung","sequence":"additional","affiliation":[{"name":"The Program for Experimental & Theoretical Modeling, Division of Hepatology, Department of Medicine, Stritch School of Medicine, Loyola University Medical Center","place":["Maywood, IL, United States"]},{"name":"Institute for Biostatistics and Mathematical Modeling, Department of Medicine, Goethe Universit\u00e4t Frankfurt","place":["Frankfurt, Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8690-9896","authenticated-orcid":true,"given":"E Fabian","family":"Cardozo-Ojeda","sequence":"additional","affiliation":[{"name":"The Program for Experimental & Theoretical Modeling, Division of Hepatology, Department of Medicine, Stritch School of Medicine, Loyola University Medical Center","place":["Maywood, IL, United States"]},{"name":"Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center","place":["Seattle, WA, United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9890-300X","authenticated-orcid":true,"given":"Sof\u00eda","family":"P\u00e9rez-del-Pulgar","sequence":"additional","affiliation":[{"name":"Liver Unit, Hospital Cl\u00ednic, IDIBAPS and CIBEREHD, University of Barcelona","place":["Barcelona, Spain"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2455-0002","authenticated-orcid":true,"given":"Alan S","family":"Perelson","sequence":"additional","affiliation":[{"name":"Theoretical Biology and Biophysics, Los Alamos National Laboratory","place":["Los Alamos, NM, United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Scott J","family":"Cotler","sequence":"additional","affiliation":[{"name":"The Program for Experimental & Theoretical Modeling, Division of Hepatology, Department of Medicine, Stritch School of Medicine, Loyola University Medical Center","place":["Maywood, IL, United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8188-1764","authenticated-orcid":true,"given":"Xavier","family":"Forns","sequence":"additional","affiliation":[{"name":"Liver Unit, Hospital Cl\u00ednic, IDIBAPS and CIBEREHD, University of Barcelona","place":["Barcelona, Spain"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5691-1557","authenticated-orcid":true,"given":"Susan L","family":"Uprichard","sequence":"additional","affiliation":[{"name":"The Program for Experimental & Theoretical Modeling, Division of Hepatology, Department of Medicine, Stritch School of Medicine, Loyola University Medical Center","place":["Maywood, IL, United States"]},{"name":"The Infectious Disease and Immunology Research Institute, Stritch School of Medicine, Loyola University Medical Center","place":["Maywood, IL, United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3357-1817","authenticated-orcid":true,"given":"Harel","family":"Dahari","sequence":"additional","affiliation":[{"name":"The Program for Experimental & Theoretical Modeling, Division of Hepatology, Department of Medicine, Stritch School of Medicine, Loyola University Medical Center","place":["Maywood, IL, United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"4374","published-online":{"date-parts":[[2021,11,3]]},"reference":[{"key":"bib1","doi-asserted-by":"publisher","first-page":"810","DOI":"10.1016\/j.jhep.2018.06.010","article-title":"Impact of DAAs on liver transplantation: Major effects on the evolution of indications and results. An ELITA study based on the ELTR registry","volume":"69","author":"Belli","year":"2018","journal-title":"Journal of Hepatology"},{"key":"bib2","doi-asserted-by":"publisher","first-page":"3252","DOI":"10.1128\/JCM.40.9.3252-3255.2002","article-title":"Detection and characterization of hepatitis C virus RNA in seminal plasma and spermatozoon fractions of semen from patients attempting medically assisted conception","volume":"40","author":"Bourlet","year":"2002","journal-title":"Journal of Clinical Microbiology"},{"key":"bib3","doi-asserted-by":"publisher","first-page":"13963","DOI":"10.1128\/JVI.79.22.13963-13973.2005","article-title":"Robust production of infectious hepatitis C virus (HCV) from stably HCV cDNA-transfected human hepatoma cells","volume":"79","author":"Cai","year":"2005","journal-title":"Journal of Virology"},{"key":"bib4","doi-asserted-by":"publisher","DOI":"10.1016\/j.mbs.2016.10.009","article-title":"On the relationship between sloppiness and identifiability","volume":"282","author":"Chis","year":"2016","journal-title":"Mathematical Biosciences"},{"key":"bib5","doi-asserted-by":"publisher","first-page":"491","DOI":"10.1016\/j.jhep.2004.12.017","article-title":"Second hepatitis C replication compartment indicated by viral dynamics during liver transplantation","volume":"42","author":"Dahari","year":"2005","journal-title":"Journal of Hepatology"},{"key":"bib6","doi-asserted-by":"publisher","first-page":"214","DOI":"10.1007\/s11901-011-0101-7","article-title":"Hepatitis C Viral Kinetics in the Era of Direct Acting Antiviral Agents and IL28B","volume":"10","author":"Dahari","year":"2011","journal-title":"Current Hepatitis Reports"},{"key":"bib7","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-74568-x","article-title":"Response guided therapy for reducing duration of direct acting antivirals in chronic hepatitis C infected patients: a Pilot study","volume":"10","author":"Etzion","year":"2020","journal-title":"Scientific Reports"},{"key":"bib8","doi-asserted-by":"publisher","first-page":"649","DOI":"10.1016\/S2468-1253(20)30081-9","article-title":"Short-course, direct-acting antivirals and ezetimibe to prevent HCV infection in recipients of organs from HCV-infected donors: a phase 3, single-centre, open-label study","volume":"5","author":"Feld","year":"2020","journal-title":"The Lancet. Gastroenterology & Hepatology"},{"key":"bib9","doi-asserted-by":"publisher","first-page":"1351","DOI":"10.1002\/hep.510240606","article-title":"Viral dynamics of hepatitis C early after orthotopic liver transplantation: evidence for rapid turnover of serum virions","volume":"24","author":"Fukumoto","year":"1996","journal-title":"Hepatology"},{"key":"bib10","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002281","article-title":"Rapid and efficient clearance of blood-borne virus by liver sinusoidal endothelium","volume":"7","author":"Ganesan","year":"2011","journal-title":"PLOS Pathogens"},{"key":"bib11","doi-asserted-by":"publisher","first-page":"680","DOI":"10.1053\/jhep.2002.31773","article-title":"Hepatitis C virus kinetics during and immediately after liver transplantation","volume":"35","author":"Garcia-Retortillo","year":"2002","journal-title":"Hepatology"},{"key":"bib12","doi-asserted-by":"publisher","first-page":"963","DOI":"10.1016\/j.jhep.2011.02.011","article-title":"Naringenin inhibits the assembly and long-term production of infectious hepatitis C virus particles through a PPAR-mediated mechanism","volume":"55","author":"Goldwasser","year":"2011","journal-title":"Journal of Hepatology"},{"key":"bib13","doi-asserted-by":"publisher","first-page":"3991","DOI":"10.1073\/pnas.1203110110","article-title":"Modeling shows that the NS5A inhibitor daclatasvir has two modes of action and yields a shorter estimate of the hepatitis C virus half-life","volume":"110","author":"Guedj","year":"2013","journal-title":"PNAS"},{"key":"bib14","doi-asserted-by":"publisher","DOI":"10.1186\/s40779-020-00288-y","article-title":"Hepatitis C: milestones from discovery to clinical cure","volume":"7","author":"Hu","year":"2020","journal-title":"Military Medical Research"},{"key":"bib15","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0134529","article-title":"Several Human Liver Cell Expressed Apolipoproteins Complement HCV Virus Production with Varying Efficacy Conferring Differential Specific Infectivity to Released Viruses","volume":"10","author":"Hueging","year":"2015","journal-title":"PLOS ONE"},{"key":"bib16","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0180719","article-title":"Rate of hepatitis C viral clearance by human livers in human patients: Liver transplantation modeling primary infection and implications for studying entry inhibition","volume":"12","author":"Hughes","year":"2017","journal-title":"PLOS ONE"},{"key":"bib17","doi-asserted-by":"publisher","first-page":"42","DOI":"10.1016\/S0140-6736(00)02435-1","article-title":"Detection of hepatitis C virus in the semen of infected men","volume":"356","author":"Leruez-Ville","year":"2000","journal-title":"Lancet"},{"key":"bib18","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1007\/7355_2018_47","article-title":"The Discovery and Development of Daclatasvir: An Inhibitor of the Hepatitis C Virus NS5A Replication Complex","volume":"Volume 32","author":"Meanwell","year":"2019","journal-title":"The Journey from Discovery to a Cure"},{"key":"bib19","doi-asserted-by":"publisher","first-page":"231","DOI":"10.1002\/(SICI)1096-9071(199703)51:3<231::AID-JMV15>3.0.CO;2-N","article-title":"Detection and genotyping of the hepatitis C RNA in tear fluid from patients with chronic hepatitis C","volume":"51","author":"Mendel","year":"1997","journal-title":"Journal of Medical Virology"},{"key":"bib20","doi-asserted-by":"publisher","first-page":"355","DOI":"10.1002\/hep.1840170302","article-title":"Hemodynamic and humoral changes after liver transplantation in patients with cirrhosis","volume":"17","author":"Navasa","year":"1993","journal-title":"Hepatology"},{"key":"bib21","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1126\/science.282.5386.103","article-title":"Hepatitis C viral dynamics in vivo and the antiviral efficacy of interferon-alpha therapy","volume":"282","author":"Neumann","year":"1998","journal-title":"Science"},{"key":"bib22","doi-asserted-by":"publisher","first-page":"120","DOI":"10.1002\/jmv.1890410207","article-title":"Demonstration of hepatitis C virus genome in saliva and urine of patients with type C hepatitis: usefulness of the single round polymerase chain reaction method for detection of the HCV genome","volume":"41","author":"Numata","year":"1993","journal-title":"Journal of Medical Virology"},{"key":"bib23","doi-asserted-by":"publisher","first-page":"529","DOI":"10.1002\/jmv.10238","article-title":"Hepatitis C virus replicates in sweat glands and is released into sweat in patients with chronic hepatitis C","volume":"68","author":"Ortiz-Movilla","year":"2002","journal-title":"Journal of Medical Virology"},{"key":"bib24","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.00504","article-title":"Environmental Stability and Infectivity of Hepatitis C Virus (HCV) in Different Human Body Fluids","volume":"9","author":"Pfaender","year":"2018","journal-title":"Frontiers in Microbiology"},{"key":"bib25","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1002\/lt.20572","article-title":"Kinetics of hepatitis C virus reinfection after liver transplantation","volume":"12","author":"Powers","year":"2006","journal-title":"Liver Transplantation"},{"key":"bib26","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-02648-6","article-title":"Glycogen synthase kinase 3\u03b2 inhibitors prevent hepatitis C virus release\/assembly through perturbation of lipid metabolism","volume":"7","author":"Sarhan","year":"2017","journal-title":"Scientific Reports"},{"key":"bib27","doi-asserted-by":"publisher","first-page":"4591","DOI":"10.1128\/JVI.02144-06","article-title":"Analysis of hepatitis C virus superinfection exclusion by using novel fluorochrome gene-tagged viral genomes","volume":"81","author":"Schaller","year":"2007","journal-title":"Journal of Virology"},{"key":"bib28","doi-asserted-by":"publisher","first-page":"4413","DOI":"10.1128\/JCM.43.9.4413-4417.2005","article-title":"Quantitative detection of hepatitis C virus (HCV) RNA in saliva and gingival crevicular fluid of HCV-infected patients","volume":"43","author":"Suzuki","year":"2005","journal-title":"Journal of Clinical Microbiology"},{"key":"bib29","doi-asserted-by":"publisher","first-page":"1040","DOI":"10.1099\/jgv.0.000726","article-title":"Usefulness of humanized cDNA-uPA\/SCID mice for the study of hepatitis B virus and hepatitis C virus virology","volume":"98","author":"Uchida","year":"2017","journal-title":"The Journal of General Virology"},{"key":"bib30","doi-asserted-by":"publisher","first-page":"791","DOI":"10.1038\/nm1268","article-title":"Production of infectious hepatitis C virus in tissue culture from a cloned viral genome","volume":"11","author":"Wakita","year":"2005","journal-title":"Nature Medicine"},{"key":"bib31","doi-asserted-by":"publisher","first-page":"672","DOI":"10.1086\/499602","article-title":"High serum hepatitis C virus (HCV) RNA load predicts the presence of HCV RNA in saliva from individuals with chronic and acute HCV infection","volume":"193","author":"Wang","year":"2006","journal-title":"The Journal of Infectious Diseases"},{"key":"bib32","doi-asserted-by":"publisher","DOI":"10.1002\/9780471729259.mc1705s18","article-title":"Cell-based hepatitis C virus infection fluorescence resonance energy transfer (FRET) assay for antiviral compound screening","volume":"Chapter 17","author":"Yu","year":"2010","journal-title":"Current Protocols in Microbiology"},{"key":"bib33","doi-asserted-by":"publisher","first-page":"5271","DOI":"10.1128\/JVI.76.10.5271-5273.2002","article-title":"The liver is a major organ for clearing simian immunodeficiency virus in rhesus monkeys","volume":"76","author":"Zhang","year":"2002","journal-title":"Journal of Virology"},{"key":"bib34","doi-asserted-by":"publisher","first-page":"9294","DOI":"10.1073\/pnas.0503596102","article-title":"Robust hepatitis C virus infection in vitro","volume":"102","author":"Zhong","year":"2005","journal-title":"PNAS"}],"container-title":["eLife"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/cdn.elifesciences.org\/articles\/65297\/elife-65297-v2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/cdn.elifesciences.org\/articles\/65297\/elife-65297-v2.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/elifesciences.org\/articles\/65297","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T21:08:21Z","timestamp":1697058501000},"score":1,"resource":{"primary":{"URL":"https:\/\/elifesciences.org\/articles\/65297"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11,3]]},"references-count":34,"alternative-id":["10.7554\/eLife.65297"],"URL":"https:\/\/doi.org\/10.7554\/elife.65297","archive":["CLOCKSS"],"relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2020.08.03.20167387","asserted-by":"subject"}],"has-review":[{"id-type":"doi","id":"10.7554\/eLife.65297.sa2","asserted-by":"object"},{"id-type":"doi","id":"10.7554\/eLife.65297.sa0","asserted-by":"object"},{"id-type":"doi","id":"10.7554\/eLife.65297.sa1","asserted-by":"object"}]},"ISSN":["2050-084X"],"issn-type":[{"value":"2050-084X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,11,3]]},"assertion":[{"value":"single anonymised","name":"peer_review_transparency","label":"Peer review transparency","group":{"name":"peer_review_taxonomy"}},{"value":"other reviewer(s), editor","name":"peer_review_interaction","label":"Peer review interaction","group":{"name":"peer_review_taxonomy"}},{"value":"review summaries, review reports, author\/editor communication, reviewer identities reviewer opt in, editor identities","name":"peer_review_published","label":"Peer review published","group":{"name":"peer_review_taxonomy"}},{"value":"open (sign in with ORCID iD required)","name":"post_publication_commenting","label":"Post publication commenting","group":{"name":"post_publication_commenting"}}],"article-number":"e65297"}}