{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T07:06:24Z","timestamp":1793171184975,"version":"4.5.0"},"reference-count":34,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2021,2,12]],"date-time":"2021-02-12T00:00:00Z","timestamp":1613088000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,2,12]],"date-time":"2021-02-12T00:00:00Z","timestamp":1613088000000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,2,12]],"date-time":"2021-02-12T00:00:00Z","timestamp":1613088000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-18-CE16 \"iReelAx\""],"award-info":[{"award-number":["ANR-18-CE16 \"iReelAx\""]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["[ANR-18-IAHU-0001"],"award-info":[{"award-number":["[ANR-18-IAHU-0001"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002915","name":"Fondation pour la Recherche M\u00e9dicale","doi-asserted-by":"publisher","award":["ECO20170637481"],"award-info":[{"award-number":["ECO20170637481"]}],"id":[{"id":"10.13039\/501100002915","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004099","name":"Ligue Contre le Cancer","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004099","id-type":"DOI","asserted-by":"publisher"}]},{"name":"UNADEV\/AVIESAN"},{"DOI":"10.13039\/100011713","name":"Worldwide Cancer Research","doi-asserted-by":"publisher","award":["grant no. 0624"],"award-info":[{"award-number":["grant no. 0624"]}],"id":[{"id":"10.13039\/100011713","id-type":"DOI","asserted-by":"publisher"}]},{"name":"LILT -Trento","award":["Program 5 per mille"],"award-info":[{"award-number":["Program 5 per mille"]}]}],"content-domain":{"domain":["elifesciences.org"],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>\n                    While zebrafish is emerging as a new model system to study human diseases, an efficient methodology to generate precise point mutations at high efficiency is still lacking. Here we show that base editors can generate C-to-T point mutations with high efficiencies without other unwanted on-target mutations. In addition, we established a new editor variant recognizing an NAA protospacer adjacent motif, expanding the base editing possibilities in zebrafish. Using these approaches, we first generated a base change in the\n                    <jats:italic>ctnnb1<\/jats:italic>\n                    gene, mimicking oncogenic an mutation of the human gene known to result in constitutive activation of endogenous Wnt signaling. Additionally, we precisely targeted several cancer-associated genes including\n                    <jats:italic>cbl<\/jats:italic>\n                    . With this last target, we created a new zebrafish dwarfism model. Together our findings expand the potential of zebrafish as a model system allowing new approaches for the endogenous modulation of cell signaling pathways and the generation of precise models of human genetic disease-associated mutations.\n                  <\/jats:p>","DOI":"10.7554\/elife.65552","type":"journal-article","created":{"date-parts":[[2021,2,12]],"date-time":"2021-02-12T08:00:57Z","timestamp":1613116857000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.65552","source":"Crossref","is-referenced-by-count":46,"title":["Precise base editing for the in vivo study of developmental signaling and human pathologies in zebrafish"],"prefix":"10.7554","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3935-6971","authenticated-orcid":true,"given":"Marion","family":"Rosello","sequence":"first","affiliation":[{"name":"Sorbonne Universit\u00e9, INSERM, CNRS, Institut de la Vision, Paris, France"},{"name":"Institut Curie, PSL Research University, Inserm U934, CNRS 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Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Paul","family":"Concordet","sequence":"additional","affiliation":[{"name":"Muse\u0301um National d\u2019Histoire Naturelle, INSERM U1154, CNRS UMR 7196, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3243-7018","authenticated-orcid":true,"given":"Shahad","family":"Albadri","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, INSERM, CNRS, Institut de la Vision, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8551-2846","authenticated-orcid":true,"given":"Filippo","family":"Del Bene","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, INSERM, CNRS, Institut de la Vision, Paris, France"},{"name":"Institut Curie, PSL Research University, Inserm U934, CNRS UMR3215, Paris, 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