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Through a combination of equilibrium and nonequilibrium molecular dynamics simulations, we identify allosteric effects and communication pathways in two prototypical class A \u03b2-lactamases, TEM-1 and KPC-2, which are important determinants of antibiotic resistance. The nonequilibrium simulations reveal pathways of communication operating over distances of 30 \u00c5 or more. Propagation of the signal occurs through cooperative coupling of loop dynamics. Notably, 50% or more of clinically relevant amino acid substitutions map onto the identified signal transduction pathways. This suggests that clinically important variation may affect, or be driven by, differences in allosteric behavior, providing a mechanism by which amino acid substitutions may affect the relationship between spectrum of activity, catalytic turnover, and potential allosteric behavior in this clinically important enzyme family. Simulations of the type presented here will help in identifying and analyzing such differences.<\/jats:p>","DOI":"10.7554\/elife.66567","type":"journal-article","created":{"date-parts":[[2021,3,23]],"date-time":"2021-03-23T09:01:50Z","timestamp":1616490110000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.66567","source":"Crossref","is-referenced-by-count":90,"title":["Allosteric communication in class A \u03b2-lactamases occurs via cooperative coupling of loop dynamics"],"prefix":"10.7554","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2136-9723","authenticated-orcid":true,"given":"Ioannis","family":"Galdadas","sequence":"first","affiliation":[{"name":"University College London, Department of Chemistry, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shen","family":"Qu","sequence":"additional","affiliation":[{"name":"University College London School of Pharmacy, Pharmaceutical and Biological Chemistry, London, United 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