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In a parasite that must adapt to the regulatory requirements of its multi-host life style, there is a need to adopt additional means to partition the distinct transcriptional signatures of the closely and tandemly arranged stage-specific genes. In this study, we report our findings in\n                    <jats:italic>T. gondii<\/jats:italic>\n                    of an m6A-dependent 3\u2019end polyadenylation serving as a transcriptional barrier at these\n                    <jats:italic>loci<\/jats:italic>\n                    . We identify the core polyadenylation complex within\n                    <jats:italic>T. gondii<\/jats:italic>\n                    and establish CPSF4 as a reader for m6A-modified mRNAs, via a YTH domain within its C-terminus, a feature which is shared with plants. We bring evidence of the specificity of this interaction both biochemically, and by determining the crystal structure at high resolution of the\n                    <jats:italic>T. gondii<\/jats:italic>\n                    CPSF4-YTH in complex with an m6A-modified RNA. We show that the loss of m6A, both at the level of its deposition or its recognition is associated with an increase in aberrantly elongated chimeric mRNAs emanating from impaired transcriptional termination, a phenotype previously noticed in the plant model\n                    <jats:italic>Arabidopsis thaliana<\/jats:italic>\n                    . Nanopore direct RNA sequencing shows the occurrence of transcriptional read-through breaching into downstream repressed stage-specific genes, in the absence of either CPSF4 or the m6A RNA methylase components in both\n                    <jats:italic>T. gondii<\/jats:italic>\n                    and\n                    <jats:italic>A. thaliana<\/jats:italic>\n                    . Taken together, our results shed light on an essential regulatory mechanism coupling the pathways of m6A metabolism directly to the cleavage and polyadenylation processes, one that interestingly seem to serve, in both\n                    <jats:italic>T. gondii<\/jats:italic>\n                    and\n                    <jats:italic>A. thaliana<\/jats:italic>\n                    , as a guardian against aberrant transcriptional read-throughs.\n                  <\/jats:p>","DOI":"10.7554\/elife.68312","type":"journal-article","created":{"date-parts":[[2021,7,15]],"date-time":"2021-07-15T08:00:45Z","timestamp":1626336045000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.68312","source":"Crossref","is-referenced-by-count":37,"title":["A plant-like mechanism coupling m6A reading to polyadenylation safeguards transcriptome integrity and developmental gene partitioning in Toxoplasma"],"prefix":"10.7554","volume":"10","author":[{"given":"Dayana C","family":"Farhat","sequence":"first","affiliation":[{"name":"IAB,Team Host-Pathogen Interactions & Immunity to Infection, INSERMU1209, CNRSUMR5309, Grenoble Alpes University, Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0465-3351","authenticated-orcid":true,"given":"Matthew W","family":"Bowler","sequence":"additional","affiliation":[{"name":"European Molecular Biology Laboratory, Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillaume","family":"Communie","sequence":"additional","affiliation":[{"name":"Institut Laue-Langevin, Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominique","family":"Pontier","sequence":"additional","affiliation":[{"name":"Laboratoire Ge\u0301nome et De\u0301veloppement des Plantes (LGDP), UMR5096, Centre National de la Recherche Scientifique (CNRS), Universite\u0301 de Perpignan via Domitia (UPVD), Perpignan, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucid","family":"Belmudes","sequence":"additional","affiliation":[{"name":"Univ. Grenoble Alpes, INSERM, CEA, UMR BioSant\u00e9 U1292, CNRS, CEA, Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline","family":"Mas","sequence":"additional","affiliation":[{"name":"Integrated Structural Biology Grenoble (ISBG) CNRS, CEA, Universit\u00e9 Grenoble Alpes, EMBL, Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlotte","family":"Corrao","sequence":"additional","affiliation":[{"name":"IAB,Team Host-Pathogen Interactions & Immunity to Infection, INSERMU1209, CNRSUMR5309, Grenoble Alpes University, Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3896-6196","authenticated-orcid":true,"given":"Yohann","family":"Cout\u00e9","sequence":"additional","affiliation":[{"name":"Univ. 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