{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T11:50:24Z","timestamp":1788609024450,"version":"build-2803163510"},"reference-count":68,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2021,7,8]],"date-time":"2021-07-08T00:00:00Z","timestamp":1625702400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"},{"start":{"date-parts":[[2021,7,8]],"date-time":"2021-07-08T00:00:00Z","timestamp":1625702400000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"},{"start":{"date-parts":[[2021,7,8]],"date-time":"2021-07-08T00:00:00Z","timestamp":1625702400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["F12-GM117582"],"award-info":[{"award-number":["F12-GM117582"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000025","name":"National Institute of Mental Health","doi-asserted-by":"publisher","award":["ZIA-MH002800"],"award-info":[{"award-number":["ZIA-MH002800"]}],"id":[{"id":"10.13039\/100000025","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000062","name":"NIDDK","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000070","name":"NIBIB","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000070","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elifesciences.org"],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>\n                    Identifying neural substrates of behavior requires defining actions in terms that map onto brain activity. Brain and muscle activity naturally correlate via the output of motor neurons, but apart from simple movements it has been difficult to define behavior in terms of muscle contractions. By mapping the musculature of the pupal fruit fly and comprehensively imaging muscle activation at single-cell resolution, we here describe a multiphasic behavioral sequence in\n                    <jats:italic>Drosophila<\/jats:italic>\n                    . Our characterization identifies a previously undescribed behavioral phase and permits extraction of major movements by a convolutional neural network. We deconstruct movements into a syllabary of co-active muscles and identify specific syllables that are sensitive to neuromodulatory manipulations. We find that muscle activity shows considerable variability, with sequential increases in stereotypy dependent upon neuromodulation. Our work provides a platform for studying whole-animal behavior, quantifying its variability across multiple spatiotemporal scales, and analyzing its neuromodulatory regulation at cellular resolution.\n                  <\/jats:p>","DOI":"10.7554\/elife.68656","type":"journal-article","created":{"date-parts":[[2021,7,8]],"date-time":"2021-07-08T08:00:22Z","timestamp":1625731222000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.68656","source":"Crossref","is-referenced-by-count":14,"title":["Pupal behavior emerges from unstructured muscle activity in response to neuromodulation in Drosophila"],"prefix":"10.7554","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3089-1786","authenticated-orcid":true,"given":"Amicia D","family":"Elliott","sequence":"first","affiliation":[{"name":"National Institute of Mental Health, National Institutes of Health, Bethesda, United States"},{"name":"National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adama","family":"Berndt","sequence":"additional","affiliation":[{"name":"National Institute of Mental Health, National Institutes of Health, Bethesda, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Houpert","sequence":"additional","affiliation":[{"name":"National Institute of Mental Health, National Institutes of Health, Bethesda, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Snehashis","family":"Roy","sequence":"additional","affiliation":[{"name":"National Institute of Mental Health, National Institutes of Health, Bethesda, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert L","family":"Scott","sequence":"additional","affiliation":[{"name":"National Institute of Mental Health, National Institutes of Health, Bethesda, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1463-9553","authenticated-orcid":true,"given":"Carson C","family":"Chow","sequence":"additional","affiliation":[{"name":"National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hari","family":"Shroff","sequence":"additional","affiliation":[{"name":"National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0612-8075","authenticated-orcid":true,"given":"Benjamin H","family":"White","sequence":"additional","affiliation":[{"name":"National Institute of Mental Health, National Institutes of Health, Bethesda, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4374","published-online":{"date-parts":[[2021,7,8]]},"reference":[{"key":"bib1","doi-asserted-by":"publisher","first-page":"413","DOI":"10.1038\/nmeth.2434","article-title":"Whole-brain functional imaging at cellular resolution using light-sheet microscopy","volume":"10","author":"Ahrens","year":"2013","journal-title":"Nature Methods"},{"key":"bib2","doi-asserted-by":"publisher","first-page":"18","DOI":"10.1016\/j.neuron.2014.09.005","article-title":"Toward a science of computational ethology","volume":"84","author":"Anderson","year":"2014","journal-title":"Neuron"},{"key":"bib3","doi-asserted-by":"publisher","first-page":"1975","DOI":"10.1016\/j.bpj.2017.02.035","article-title":"Visualizing Calcium Flux in Freely Moving Nematode Embryos","volume":"112","author":"Ardiel","year":"2017","journal-title":"Biophysical journal"},{"key":"bib4","first-page":"57","article-title":"Staging the metamorphosis of Drosophila melanogaster","volume":"66","author":"Bainbridge","year":"1981","journal-title":"Journal of embryology and experimental morphology"},{"key":"bib5","doi-asserted-by":"publisher","DOI":"10.1098\/rsif.2014.0672","article-title":"Mapping the stereotyped behaviour of freely moving fruit flies","volume":"11","author":"Berman","year":"2014","journal-title":"Journal of the Royal Society Interface"},{"key":"bib6","doi-asserted-by":"publisher","first-page":"18","DOI":"10.1038\/nrn2759","article-title":"Mechanisms underlying spontaneous patterned activity in developing neural circuits","volume":"11","author":"Blankenship","year":"2010","journal-title":"Nature reviews. Neuroscience"},{"key":"bib7","doi-asserted-by":"publisher","first-page":"299","DOI":"10.1007\/BF00656847","article-title":"Imaginal ecdysis of cricket (Teleogryllus-Oceanicus). 1. organization of motor programs and roles of central and sensory control","volume":"115","author":"Carlson","year":"1977","journal-title":"Journal of Comparative Physiology"},{"key":"bib8","doi-asserted-by":"publisher","first-page":"1006","DOI":"10.1126\/science.841322","article-title":"Ecdysis: neural orchestration of a complex behavioral performance","volume":"195","author":"Carlson","year":"1977","journal-title":"Science"},{"key":"bib9","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.38554","article-title":"MDN brain descending neurons coordinately activate backward and inhibit forward locomotion","volume":"7","author":"Carreira-Rosario","year":"2018","journal-title":"eLife"},{"key":"bib10","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1002\/dvg.1020050206","article-title":"Pupation in Drosophila Melanogaster and the effect of theLethalcryptocephal mutation","volume":"5","author":"Chadfield","year":"1984","journal-title":"Developmental Genetics"},{"key":"bib11","doi-asserted-by":"publisher","first-page":"1140","DOI":"10.1016\/j.peptides.2008.02.013","article-title":"Evidence for postsynaptic modulation of muscle contraction by a Drosophila neuropeptide","volume":"29","author":"Clark","year":"2008","journal-title":"Peptides"},{"key":"bib12","doi-asserted-by":"publisher","DOI":"10.1186\/s13064-018-0103-z","article-title":"Neural circuits driving larval locomotion in Drosophila","volume":"13","author":"Clark","year":"2018","journal-title":"Neural development"},{"key":"bib13","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.28158","article-title":"Rapid whole brain imaging of neural activity in freely behaving larval zebrafish (Danio rerio)","volume":"6","author":"Cong","year":"2017","journal-title":"eLife"},{"key":"bib14","doi-asserted-by":"publisher","first-page":"3707","DOI":"10.1242\/dev.026773","article-title":"The development of motor coordination in Drosophila embryos","volume":"135","author":"Crisp","year":"2008","journal-title":"Development"},{"key":"bib15","doi-asserted-by":"publisher","first-page":"10445","DOI":"10.1523\/JNEUROSCI.0346-11.2011","article-title":"Endogenous patterns of activity are required for the maturation of a motor network","volume":"31","author":"Crisp","year":"2011","journal-title":"Journal of Neuroscience"},{"key":"bib16","doi-asserted-by":"publisher","first-page":"297","DOI":"10.1038\/nmeth.1310","article-title":"Automated monitoring and analysis of social behavior in Drosophila","volume":"6","author":"Dankert","year":"2009","journal-title":"Nature methods"},{"key":"bib17","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1016\/j.neuron.2019.09.038","article-title":"Computational Neuroethology: A Call to Action","volume":"104","author":"Datta","year":"2019","journal-title":"Neuron"},{"key":"bib18","doi-asserted-by":"publisher","first-page":"243","DOI":"10.1146\/annurev.en.39.010194.001331","article-title":"Metamorphosis behavior of flies","volume":"39","author":"Denlinger","year":"1994","journal-title":"Annual review of entomology"},{"key":"bib19","doi-asserted-by":"publisher","first-page":"1410","DOI":"10.1016\/j.celrep.2015.01.059","article-title":"Plug-and-play genetic access to Drosophila cell types using exchangeable exon cassettes","volume":"10","author":"Diao","year":"2015","journal-title":"Cell reports"},{"key":"bib20","doi-asserted-by":"publisher","first-page":"175","DOI":"10.1534\/genetics.115.182121","article-title":"The splice isoforms of the Drosophila ecdysis triggering hormone receptor have developmentally distinct roles","volume":"202","author":"Diao","year":"2016","journal-title":"Genetics"},{"key":"bib21","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.29797","article-title":"Neuromodulatory connectivity defines the structure of a behavioral neural network","volume":"6","author":"Diao","year":"2017","journal-title":"eLife"},{"key":"bib22","first-page":"1","volume-title":"Hormones, Brain, and Behavior","author":"Ewer","year":"2002"},{"key":"bib23","doi-asserted-by":"publisher","first-page":"303","DOI":"10.1002\/neu.480170405","article-title":"Effects of octopamine and forskolin on excitatory junction potentials of developing and adult moth muscle","volume":"17","author":"Fitch","year":"1986","journal-title":"Journal of neurobiology"},{"key":"bib24","doi-asserted-by":"publisher","first-page":"12460","DOI":"10.1523\/JNEUROSCI.0222-12.2012","article-title":"Characterization of Drosophila larval crawling at the level of organism, segment, and somatic body wall musculature","volume":"32","author":"Heckscher","year":"2012","journal-title":"Journal of Neuroscience"},{"key":"bib25","doi-asserted-by":"publisher","first-page":"E5351","DOI":"10.1073\/pnas.1515982112","article-title":"Automated measurement of mouse social behaviors using depth sensing, video tracking, and machine learning","volume":"112","author":"Hong","year":"2015","journal-title":"PNAS"},{"key":"bib26","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1111\/j.1365-3032.1980.tb00210.x","article-title":"Imaginal ecdysis of the desert Locust, Schistocerca-Gregaria. 1. description of the behavior","volume":"5","author":"Hughes","year":"1980","journal-title":"Physiological Entomology"},{"key":"bib27","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1111\/j.1365-3032.1980.tb00211.x","article-title":"Imaginal ecdysis of the desert Locust, Schistocerca-Gregaria. 2. Motor-Activity underlying the Pre-Emergence and emergence behavior","volume":"5","author":"Hughes","year":"1980","journal-title":"Physiological Entomology"},{"key":"bib28","doi-asserted-by":"publisher","first-page":"141","DOI":"10.1111\/j.1365-3032.1980.tb00221.x","article-title":"The imaginal ecdysis of the desert Locust, Schistocerca-Gregaria. 3. Motor-Activity underlying the expansional and Post-Expansional behavior","volume":"5","author":"Hughes","year":"1980","journal-title":"Physiological Entomology"},{"key":"bib29","doi-asserted-by":"publisher","first-page":"70","DOI":"10.1016\/j.cub.2019.11.026","article-title":"Probabilistic models of larval zebrafish behavior reveal structure on many scales","volume":"30","author":"Johnson","year":"2020","journal-title":"Current Biology"},{"key":"bib30","doi-asserted-by":"publisher","first-page":"64","DOI":"10.1038\/nmeth.2281","article-title":"JAABA: interactive machine learning for automatic annotation of animal behavior","volume":"10","author":"Kabra","year":"2013","journal-title":"Nature methods"},{"key":"bib31","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1007\/BF02582971","article-title":"Maturation of the flight motor pattern without movement inManduca sexta","volume":"130","author":"Kammer","year":"1979","journal-title":"Journal of Comparative Physiology A"},{"key":"bib32","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1242\/jeb.65.1.65","article-title":"Adult motor patterns produced by moth pupae during development","volume":"65","author":"Kammer","year":"1976","journal-title":"Journal of Experimental Biology"},{"key":"bib33","doi-asserted-by":"publisher","first-page":"1247","DOI":"10.1242\/jeb.056549","article-title":"The diversity of hydrostatic skeletons","volume":"215","author":"Kier","year":"2012","journal-title":"Journal of Experimental Biology"},{"key":"bib34","doi-asserted-by":"publisher","first-page":"1395","DOI":"10.1016\/j.cub.2006.06.027","article-title":"A command chemical triggers an innate behavior by sequential activation of multiple peptidergic ensembles","volume":"16","author":"Kim","year":"2006","journal-title":"Current Biology"},{"key":"bib35","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1005513","article-title":"Rescheduling behavioral subunits of a fixed action pattern by genetic manipulation of peptidergic signaling","volume":"11","author":"Kim","year":"2015","journal-title":"PLOS Genetics"},{"key":"bib36","article-title":"Adam: a method for stochastic optimization","volume-title":"arXiv","author":"Kingma","year":"2015","unstructured":"Kingma DP, Ba JL. 2015. Adam: a method for stochastic optimization. arXiv. https:\/\/arxiv.org\/abs\/1412.6980."},{"key":"bib37","doi-asserted-by":"publisher","first-page":"227","DOI":"10.1002\/neu.480160307","article-title":"Octopamine enhances neuromuscular transmission in developing and adult moths, Manduca sexta","volume":"16","author":"Klaassen","year":"1985","journal-title":"Journal of Neurobiology"},{"key":"bib38","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-10695-y","article-title":"Regulation of forward and backward locomotion through intersegmental feedback circuits in Drosophila larvae","volume":"10","author":"Kohsaka","year":"2019","journal-title":"Nature Communications"},{"key":"bib39","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/978-1-4612-2104-3","volume-title":"Biomechanics and Neural Control of Posture and Movement","author":"Kristan","year":"2000"},{"key":"bib40","doi-asserted-by":"publisher","first-page":"6819","DOI":"10.1523\/JNEUROSCI.5301-11.2012","article-title":"Genetic analysis of ecdysis behavior in Drosophila reveals partially overlapping functions of two unrelated neuropeptides","volume":"32","author":"Lahr","year":"2012","journal-title":"Journal of Neuroscience"},{"key":"bib41","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0000041","article-title":"Embryonic origins of a motor system: motor dendrites form a myotopic map in Drosophila","volume":"1","author":"Landgraf","year":"2003","journal-title":"PLOS Biology"},{"key":"bib42","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms8924","article-title":"Whole-central nervous system functional imaging in larval Drosophila","volume":"6","author":"Lemon","year":"2015","journal-title":"Nature Communications"},{"key":"bib43","doi-asserted-by":"publisher","first-page":"11624","DOI":"10.1523\/JNEUROSCI.0410-10.2010","article-title":"Distinct presynaptic and postsynaptic dismantling processes of Drosophila neuromuscular junctions during metamorphosis","volume":"30","author":"Liu","year":"2010","journal-title":"Journal of Neuroscience"},{"key":"bib44","doi-asserted-by":"publisher","first-page":"425","DOI":"10.1016\/j.neuron.2006.08.028","article-title":"Refined spatial manipulation of neuronal function by combinatorial restriction of transgene expression","volume":"52","author":"Luan","year":"2006","journal-title":"Neuron"},{"key":"bib45","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.07892","article-title":"A quantitative framework for whole-body coordination reveals specific deficits in freely walking ataxic mice","volume":"4","author":"Machado","year":"2015","journal-title":"eLife"},{"key":"bib46","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.neuron.2012.09.010","article-title":"Neuromodulation of neuronal circuits: back to the future","volume":"76","author":"Marder","year":"2012","journal-title":"Neuron"},{"key":"bib47","doi-asserted-by":"publisher","first-page":"1281","DOI":"10.1038\/s41593-018-0209-y","article-title":"DeepLabCut: markerless pose estimation of user-defined body parts with deep learning","volume":"21","author":"Mathis","year":"2018","journal-title":"Nature Neuroscience"},{"key":"bib48","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.19686","article-title":"Stereotyped responses of Drosophila peptidergic neuronal ensemble depend on downstream neuromodulators","volume":"5","author":"Mena","year":"2016","journal-title":"eLife"},{"key":"bib49","doi-asserted-by":"publisher","first-page":"E1074","DOI":"10.1073\/pnas.1507110112","article-title":"Whole-brain calcium imaging with cellular resolution in freely behaving Caenorhabditis elegans","volume":"113","author":"Nguyen","year":"2016","journal-title":"PNAS"},{"key":"bib50","doi-asserted-by":"publisher","first-page":"94","DOI":"10.1016\/S0959-4388(99)80012-9","article-title":"The origin of spontaneous activity in developing networks of the vertebrate nervous system","volume":"9","author":"O'Donovan","year":"1999","journal-title":"Current Opinion in Neurobiology"},{"key":"bib51","doi-asserted-by":"publisher","first-page":"2645","DOI":"10.1242\/dev.00503","article-title":"Targeted ablation of CCAP neuropeptide-containing neurons of Drosophila causes specific defects in execution and circadian timing of ecdysis behavior","volume":"130","author":"Park","year":"2003","journal-title":"Development"},{"key":"bib52","doi-asserted-by":"publisher","first-page":"R173","DOI":"10.1152\/ajpregu.1982.242.3.R173","article-title":"The good enough calculi of evolving control systems: evolution is not engineering","volume":"242","author":"Partridge","year":"1982","journal-title":"American Journal of Physiology-Regulatory, Integrative and Comparative Physiology"},{"key":"bib53","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1016\/S0074-7742(06)75004-8","article-title":"Organization of the efferent system and structure of neuromuscular junctions in Drosophila","volume":"75","author":"Prokop","year":"2006","journal-title":"Fly Neuromuscular Junction: Structure and Function"},{"key":"bib54","doi-asserted-by":"publisher","first-page":"2564","DOI":"10.1152\/jn.00731.2015","article-title":"Imaging fictive locomotor patterns in larval Drosophila","volume":"114","author":"Pulver","year":"2015","journal-title":"Journal of Neurophysiology"},{"key":"bib55","doi-asserted-by":"publisher","first-page":"351","DOI":"10.1002\/jmor.1050590207","article-title":"The metamorphosis of Drosophila Melanogaster, including an accurately timed account of the principal morphological changes","volume":"59","author":"Robertson","year":"1936","journal-title":"Journal of Morphology"},{"key":"bib56","doi-asserted-by":"publisher","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: an open-source platform for biological-image analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nature methods"},{"key":"bib57","doi-asserted-by":"publisher","DOI":"10.1109\/CVPR.1997.609286","article-title":"Going deeper with convolutions","author":"Szegedy","year":"2015","unstructured":"Szegedy C, Liu W, Jia Y, Sermanet P, Reed S, Anguelov D, Erhan D, Vanhoucke V, Rabinovich A. 2015. Going deeper with convolutions. IEEE Conference on Computer Vision and Pattern Recognition."},{"key":"bib58","doi-asserted-by":"publisher","first-page":"1807","DOI":"10.1016\/j.cub.2019.05.012","article-title":"Mapping the Whole-Body Muscle Activity of Hydra vulgaris","volume":"29","author":"Szymanski","year":"2019","journal-title":"Current biology : CB"},{"key":"bib59","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.38740","article-title":"Sensorimotor pathway controlling stopping behavior during chemotaxis in the Drosophila Melanogaster larva","volume":"7","author":"Tastekin","year":"2018","journal-title":"eLife"},{"key":"bib60","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/S0083-6729(05)73001-6","article-title":"Hormonal control of insect ecdysis: endocrine cascades for coordinating behavior with physiology","volume":"73","author":"Truman","year":"2005","journal-title":"Vitamins and hormones"},{"key":"bib61","doi-asserted-by":"publisher","first-page":"935","DOI":"10.1016\/j.cub.2019.01.060","article-title":"Characterization of Proprioceptive System Dynamics in Behaving Drosophila Larvae Using High-Speed Volumetric Microscopy","volume":"29","author":"Vaadia","year":"2019","journal-title":"Current biology : CB"},{"key":"bib62","doi-asserted-by":"publisher","first-page":"3147","DOI":"10.1242\/dev.064105","article-title":"Retrograde BMP signaling controls Drosophila behavior through regulation of a peptide hormone battery","volume":"138","author":"Veverytsa","year":"2011","journal-title":"Development"},{"key":"bib63","article-title":"Classify muscle activity from Ca images of flies","volume-title":"Software Heritage","author":"White","year":"2021","unstructured":"White BH. 2021. Classify muscle activity from Ca images of flies. Software Heritage. swh:1:rev:66456f6ff61e8faa9fe4b442b91ef3fce3b178f9. https:\/\/archive.softwareheritage.org\/swh:1:rev:66456f6ff61e8faa9fe4b442b91ef3fce3b178f9."},{"key":"bib64","doi-asserted-by":"publisher","first-page":"363","DOI":"10.1146\/annurev-ento-011613-162028","article-title":"Neural and hormonal control of postecdysial behaviors in insects","volume":"59","author":"White","year":"2014","journal-title":"Annual review of entomology"},{"key":"bib65","doi-asserted-by":"publisher","first-page":"1121","DOI":"10.1016\/j.neuron.2015.11.031","article-title":"Mapping Sub-Second Structure in Mouse Behavior","volume":"88","author":"Wiltschko","year":"2015","journal-title":"Neuron"},{"key":"bib66","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.51781","article-title":"A multilayer circuit architecture for the generation of distinct locomotor behaviors in Drosophila","volume":"8","author":"Zarin","year":"2019","journal-title":"eLife"},{"key":"bib67","doi-asserted-by":"publisher","first-page":"917","DOI":"10.1016\/0022-1910(86)90139-3","article-title":"Pupal ecdysis in flies: mechanisms of evagination of the head and expansion of the thoracic appendages","volume":"32","author":"Zdarek","year":"1986","journal-title":"Journal of Insect Physiology"},{"key":"bib68","doi-asserted-by":"publisher","first-page":"253","DOI":"10.1016\/C2009-0-62089-4","volume-title":"Insect Endocrinology","author":"Zitnan","year":"2012"}],"container-title":["eLife"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/cdn.elifesciences.org\/articles\/68656\/elife-68656-v2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/cdn.elifesciences.org\/articles\/68656\/elife-68656-v2.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/elifesciences.org\/articles\/68656","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T21:16:39Z","timestamp":1697058999000},"score":1,"resource":{"primary":{"URL":"https:\/\/elifesciences.org\/articles\/68656"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,7,8]]},"references-count":68,"alternative-id":["10.7554\/eLife.68656"],"URL":"https:\/\/doi.org\/10.7554\/elife.68656","archive":["CLOCKSS"],"relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2021.03.10.434785","asserted-by":"subject"},{"id-type":"doi","id":"10.1101\/2021.03.10.434785","asserted-by":"object"}],"is-supplemented-by":[{"id-type":"doi","id":"10.6084\/m9.figshare.c.5489637","asserted-by":"subject"}],"has-review":[{"id-type":"doi","id":"10.7554\/eLife.68656.sa1","asserted-by":"object"},{"id-type":"doi","id":"10.7554\/eLife.68656.sa2","asserted-by":"object"}]},"ISSN":["2050-084X"],"issn-type":[{"value":"2050-084X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,7,8]]},"assertion":[{"value":"single anonymised","name":"peer_review_transparency","label":"Peer review transparency","group":{"name":"peer_review_taxonomy"}},{"value":"other reviewer(s), editor","name":"peer_review_interaction","label":"Peer review interaction","group":{"name":"peer_review_taxonomy"}},{"value":"review summaries, review reports, author\/editor communication, reviewer identities reviewer opt in, editor identities","name":"peer_review_published","label":"Peer review published","group":{"name":"peer_review_taxonomy"}},{"value":"open (sign in with ORCID iD required)","name":"post_publication_commenting","label":"Post publication commenting","group":{"name":"post_publication_commenting"}}],"article-number":"e68656"}}