{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T01:10:41Z","timestamp":1792804241118,"version":"4.5.0"},"reference-count":51,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2021,9,6]],"date-time":"2021-09-06T00:00:00Z","timestamp":1630886400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,9,6]],"date-time":"2021-09-06T00:00:00Z","timestamp":1630886400000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,9,6]],"date-time":"2021-09-06T00:00:00Z","timestamp":1630886400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100013290","name":"National Key Research and Development Program of China Stem Cell and Translational Research","doi-asserted-by":"crossref","award":["2018YFC1005002"],"award-info":[{"award-number":["2018YFC1005002"]}],"id":[{"id":"10.13039\/501100013290","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82070482"],"award-info":[{"award-number":["82070482"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81771971"],"award-info":[{"award-number":["81771971"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81772007"],"award-info":[{"award-number":["81772007"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["21734003"],"award-info":[{"award-number":["21734003"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["51927805"],"award-info":[{"award-number":["51927805"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elifesciences.org"],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2021,8,18]]},"abstract":"<jats:sec id=\"abs1\">\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Bicuspid aortic valve (BAV) is the most common congenital cardiovascular disease in general population and is frequently associated with the development of thoracic aortic aneurysm (TAA). There is no effective strategy to intervene with TAA progression due to an incomplete understanding of the pathogenesis. Insufficiency of NOTCH1 expression is highly related to BAV-TAA, but the underlying mechanism remains to be clarified.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"abs2\">\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>A comparative proteomics analysis was used to explore the biological differences between non-diseased and BAV-TAA aortic tissues. A microfluidics-based aorta smooth muscle-on-a-chip model was constructed to evaluate the effect of NOTCH1 deficiency on contractile phenotype and mitochondrial dynamics of human aortic smooth muscle cells (HAoSMCs).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"abs3\">\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>Protein analyses of human aortic tissues showed the insufficient expression of NOTCH1 and impaired mitochondrial dynamics in BAV-TAA. HAoSMCs with NOTCH1-knockdown exhibited reduced contractile phenotype and were accompanied by attenuated mitochondrial fusion. Furthermore, we identified that mitochondrial fusion activators (leflunomide and teriflunomide) or mitochondrial fission inhibitor (Mdivi-1) partially rescued the disorders of mitochondrial dynamics in HAoSMCs derived from BAV-TAA patients.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"abs4\">\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>The aorta smooth muscle-on-a-chip model simulates the human pathophysiological parameters of aorta biomechanics and provides a platform for molecular mechanism studies of aortic disease and related drug screening. This aorta smooth muscle-on-a-chip model and human tissue proteomic analysis revealed that impaired mitochondrial dynamics could be a potential therapeutic target for BAV-TAA.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"abs5\">\n                    <jats:title>Funding:<\/jats:title>\n                    <jats:p>National Key R and D Program of China, National Natural Science Foundation of China, Shanghai Municipal Science and Technology Major Project, Shanghai Science and Technology Commission, and Shanghai Municipal Education Commission.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.7554\/elife.69310","type":"journal-article","created":{"date-parts":[[2021,9,6]],"date-time":"2021-09-06T14:05:44Z","timestamp":1630937144000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.69310","source":"Crossref","is-referenced-by-count":47,"title":["Aorta smooth muscle-on-a-chip reveals impaired mitochondrial dynamics as a therapeutic target for aortic aneurysm in bicuspid aortic valve disease"],"prefix":"10.7554","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5421-9820","authenticated-orcid":true,"given":"Mieradilijiang","family":"Abudupataer","sequence":"first","affiliation":[{"name":"Department of Cardiac Surgery and Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5057-9434","authenticated-orcid":true,"given":"Shichao","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery and Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiqiang","family":"Yan","sequence":"additional","affiliation":[{"name":"Institutes of Biomedical Sciences and the Shanghai Key Laboratory of Medical Epigenetics, Shanghai Medical College, Fudan University","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kehua","family":"Xu","sequence":"additional","affiliation":[{"name":"Institutes of Biomedical Sciences and the Shanghai Key Laboratory of Medical Epigenetics, Shanghai Medical College, Fudan University","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingjing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institutes of Biomedical Sciences and the Shanghai Key Laboratory of Medical Epigenetics, Shanghai Medical College, Fudan University","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaman","family":"Luo","sequence":"additional","affiliation":[{"name":"Institutes of Biomedical Sciences and the Shanghai Key Laboratory of Medical Epigenetics, Shanghai Medical College, Fudan University","place":["Shanghai, China"]},{"name":"The State Key Laboratory of Molecular Engineering of Polymers, Fudan University","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenrui","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery and Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md Fazle","family":"Alam","sequence":"additional","affiliation":[{"name":"Institutes of Biomedical Sciences and the Shanghai Key Laboratory of Medical Epigenetics, Shanghai Medical College, Fudan University","place":["Shanghai, China"]},{"name":"The State Key Laboratory of Molecular Engineering of Polymers, Fudan University","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuyi","family":"Tang","sequence":"additional","affiliation":[{"name":"Institutes of Biomedical Sciences and the Shanghai Key Laboratory of Medical Epigenetics, Shanghai Medical College, Fudan University","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Huang","sequence":"additional","affiliation":[{"name":"Institutes of Biomedical Sciences and the Shanghai Key Laboratory of Medical Epigenetics, Shanghai Medical College, Fudan University","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nan","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery and Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Wang","sequence":"additional","affiliation":[{"name":"Institutes of Biomedical Sciences and the Shanghai Key Laboratory of Medical Epigenetics, Shanghai Medical College, Fudan University","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoquan","family":"Yan","sequence":"additional","affiliation":[{"name":"Institutes of Biomedical Sciences and the Shanghai Key Laboratory of Medical Epigenetics, Shanghai Medical College, Fudan University","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery and Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University","place":["Shanghai, China"]},{"name":"Institutes of Biomedical Sciences and the Shanghai Key Laboratory of Medical Epigenetics, Shanghai Medical College, Fudan University","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Lai","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery and Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunsheng","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery and Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2391-4193","authenticated-orcid":true,"given":"Kai","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery and Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6928-0416","authenticated-orcid":true,"given":"Weijia","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery and Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University","place":["Shanghai, China"]},{"name":"Institutes of Biomedical Sciences and the Shanghai Key Laboratory of Medical Epigenetics, Shanghai Medical College, Fudan University","place":["Shanghai, China"]},{"name":"The State Key Laboratory of Molecular Engineering of Polymers, Fudan University","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4374","published-online":{"date-parts":[[2021,9,6]]},"reference":[{"key":"bib1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1002\/adhm.201700506","article-title":"Organ-On-A-Chip platforms: a convergence of advanced materials, cells, and microscale technologies","volume":"2","author":"Ahadian","year":"2018","journal-title":"Advanced Healthcare Materials"},{"key":"bib2","doi-asserted-by":"publisher","first-page":"2369","DOI":"10.1253\/circj.CJ-16-0739","article-title":"Decreased aortic elasticity in children with marfan syndrome or Loeys-Dietz syndrome","volume":"80","author":"Akazawa","year":"2016","journal-title":"Circulation Journal"},{"key":"bib3","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-32170-2","article-title":"Deregulation of Notch1 pathway and circulating endothelial progenitor cell (EPC) number in patients with bicuspid aortic valve with and without ascending aorta aneurysm","volume":"8","author":"Balistreri","year":"2018","journal-title":"Scientific Reports"},{"key":"bib4","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.114.001536","article-title":"Longitudinal and circumferential strain of the proximal aorta","volume":"3","author":"Bell","year":"2014","journal-title":"Journal of the American Heart Association"},{"key":"bib5","doi-asserted-by":"publisher","first-page":"872","DOI":"10.1038\/ncb1161","article-title":"Dysregulation of HSG triggers vascular proliferative disorders","volume":"6","author":"Chen","year":"2004","journal-title":"Nature Cell Biology"},{"key":"bib6","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-018-1083-y","article-title":"Positive feedback loop between mitochondrial fission and Notch signaling promotes survivin-mediated survival of TNBC cells","volume":"9","author":"Chen","year":"2018","journal-title":"Cell Death & Disease"},{"key":"bib7","doi-asserted-by":"publisher","first-page":"2780","DOI":"10.1161\/CIR.0b013e3181e4d033","article-title":"Surgical management of descending thoracic aortic disease: open and endovascular approaches","volume":"121","author":"Coady","year":"2010","journal-title":"Circulation"},{"key":"bib8","doi-asserted-by":"publisher","first-page":"971","DOI":"10.1093\/cvr\/cvaa133","article-title":"Targeting mitochondrial fission as a potential therapeutic for abdominal aortic aneurysm","volume":"117","author":"Cooper","year":"2021","journal-title":"Cardiovascular Research"},{"key":"bib9","doi-asserted-by":"publisher","first-page":"S186","DOI":"10.1161\/CIRCULATIONAHA.112.000363","article-title":"Reproducible porcine model of thoracic aortic aneurysm","volume":"128","author":"Eckhouse","year":"2013","journal-title":"Circulation"},{"key":"bib10","doi-asserted-by":"publisher","first-page":"270","DOI":"10.1038\/nature03940","article-title":"Mutations in NOTCH1 cause aortic valve disease","volume":"437","author":"Garg","year":"2005","journal-title":"Nature"},{"key":"bib11","doi-asserted-by":"publisher","first-page":"327","DOI":"10.1161\/CIRCRESAHA.113.300675","article-title":"Genetics of thoracic aortic aneurysm: at the crossroad of transforming growth factor-\u03b2 signaling and vascular smooth muscle cell contractility","volume":"113","author":"Gillis","year":"2013","journal-title":"Circulation Research"},{"key":"bib12","doi-asserted-by":"publisher","first-page":"1725","DOI":"10.1016\/j.jacc.2014.08.025","article-title":"Thoracic aortic aneurysm and dissection","volume":"64","author":"Goldfinger","year":"2014","journal-title":"Journal of the American College of Cardiology"},{"key":"bib13","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1093\/ejcts\/ezy464","article-title":"Defective NOTCH signalling drives smooth muscle cell death and differentiation in bicuspid aortic valve aortopathy","volume":"56","author":"Harrison","year":"2019","journal-title":"European Journal of Cardio-Thoracic Surgery"},{"key":"bib14","doi-asserted-by":"publisher","first-page":"2741","DOI":"10.1161\/CIRCULATIONAHA.118.034365","article-title":"Proteomic architecture of human coronary and aortic atherosclerosis","volume":"137","author":"Herrington","year":"2018","journal-title":"Circulation"},{"key":"bib15","doi-asserted-by":"publisher","first-page":"353","DOI":"10.1172\/JCI30070","article-title":"An essential role for notch in neural crest during cardiovascular development and smooth muscle differentiation","volume":"117","author":"High","year":"2007","journal-title":"Journal of Clinical Investigation"},{"key":"bib16","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.60145","article-title":"Global and local tension measurements in biomimetic skeletal muscle tissues reveals early mechanical homeostasis","volume":"10","author":"Hofemeier","year":"2021","journal-title":"eLife"},{"key":"bib17","doi-asserted-by":"publisher","first-page":"1662","DOI":"10.1126\/science.1188302","article-title":"Reconstituting organ-level lung functions on a chip","volume":"328","author":"Huh","year":"2010","journal-title":"Science"},{"key":"bib18","doi-asserted-by":"publisher","first-page":"157","DOI":"10.1016\/S0022-4804(03)00193-8","article-title":"A murine model of thoracic aortic aneurysms","volume":"115","author":"Ikonomidis","year":"2003","journal-title":"Journal of Surgical Research"},{"key":"bib19","doi-asserted-by":"publisher","first-page":"1105","DOI":"10.1016\/j.cell.2016.02.049","article-title":"Reverse engineering human pathophysiology with Organs-on-Chips","volume":"164","author":"Ingber","year":"2016","journal-title":"Cell"},{"key":"bib20","doi-asserted-by":"publisher","first-page":"2516","DOI":"10.1161\/CIRCULATIONAHA.116.009762","article-title":"Hereditary influence in thoracic aortic aneurysm and dissection","volume":"133","author":"Isselbacher","year":"2016","journal-title":"Circulation"},{"key":"bib21","doi-asserted-by":"publisher","first-page":"734","DOI":"10.1126\/science.1241359","article-title":"Mitochondrial fusion directs cardiomyocyte differentiation via calcineurin and notch signaling","volume":"342","author":"Kasahara","year":"2013","journal-title":"Science"},{"key":"bib22","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.91353","article-title":"Notch1 haploinsufficiency causes ascending aortic aneurysms in mice","volume":"2","author":"Koenig","year":"2017","journal-title":"JCI Insight"},{"key":"bib23","doi-asserted-by":"publisher","first-page":"1374","DOI":"10.1161\/CIRCULATIONAHA.120.046528","article-title":"Single-Cell transcriptome analysis reveals dynamic cell populations and differential gene expression patterns in control and aneurysmal human aortic tissue","volume":"142","author":"Li","year":"2020","journal-title":"Circulation"},{"key":"bib24","doi-asserted-by":"publisher","first-page":"648","DOI":"10.1002\/jcb.25016","article-title":"Regulation of mitochondrial morphology by positive feedback interaction between pkc\u03b4 and Drp1 in vascular smooth muscle cell","volume":"116","author":"Lim","year":"2015","journal-title":"Journal of Cellular Biochemistry"},{"key":"bib25","doi-asserted-by":"publisher","first-page":"631","DOI":"10.1161\/CIRCRESAHA.118.312439","article-title":"Pharmacologic management of aneurysms","volume":"124","author":"Lindeman","year":"2019","journal-title":"Circulation Research"},{"key":"bib26","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2019.165631","article-title":"Notch signaling in the pathogenesis of thoracic aortic aneurysms: a bridge between embryonic and adult states","volume":"1866","author":"Malashicheva","year":"2020","journal-title":"Biochimica Et Biophysica Acta (BBA) - Molecular Basis of Disease"},{"key":"bib27","doi-asserted-by":"publisher","first-page":"62","DOI":"10.1093\/icvts\/ivv074","article-title":"Phenotypic switching of vascular smooth muscle cells in animal model of rat thoracic aortic aneurysm","volume":"21","author":"Mao","year":"2015","journal-title":"Interactive CardioVascular and Thoracic Surgery"},{"key":"bib28","doi-asserted-by":"publisher","first-page":"578","DOI":"10.1093\/cvr\/cvy006","article-title":"From genetics to response to injury: vascular smooth muscle cells in aneurysms and dissections of the ascending aorta","volume":"114","author":"Michel","year":"2018","journal-title":"Cardiovascular Research"},{"key":"bib29","doi-asserted-by":"publisher","first-page":"1104","DOI":"10.1001\/jama.2011.1286","article-title":"Incidence of aortic complications in patients with bicuspid aortic valves","volume":"306","author":"Michelena","year":"2011","journal-title":"Jama"},{"key":"bib30","doi-asserted-by":"publisher","first-page":"283","DOI":"10.1146\/annurev.genom.8.080706.092303","article-title":"Genetic basis of thoracic aortic aneurysms and dissections: focus on smooth muscle cell contractile dysfunction","volume":"9","author":"Milewicz","year":"2008","journal-title":"Annual Review of Genomics and Human Genetics"},{"key":"bib31","doi-asserted-by":"publisher","first-page":"268","DOI":"10.1016\/j.chembiol.2017.12.001","article-title":"Identification of new activators of mitochondrial fusion reveals a link between mitochondrial morphology and pyrimidine metabolism","volume":"25","author":"Miret-Casals","year":"2018","journal-title":"Cell Chemical Biology"},{"key":"bib32","doi-asserted-by":"publisher","first-page":"1029","DOI":"10.1016\/j.jvs.2008.11.056","article-title":"Circumferential and longitudinal cyclic strain of the human thoracic aorta: Age-related changes","volume":"49","author":"Morrison","year":"2009","journal-title":"Journal of Vascular Surgery"},{"key":"bib33","doi-asserted-by":"publisher","first-page":"1960","DOI":"10.1161\/RES.0000000000000104","article-title":"Mitochondrial function, biology, and role in disease: a scientific statement from the american heart association","volume":"118","author":"Murphy","year":"2016","journal-title":"Circulation Research"},{"key":"bib34","doi-asserted-by":"publisher","first-page":"1468","DOI":"10.1161\/01.RES.0000229683.81357.26","article-title":"Smooth muscle alpha-actin is a direct target of notch\/CSL","volume":"98","author":"Noseda","year":"2006","journal-title":"Circulation Research"},{"key":"bib35","doi-asserted-by":"publisher","first-page":"2091","DOI":"10.1161\/CIRCULATIONAHA.120.051171","article-title":"Extracellular tuning of mitochondrial respiration leads to aortic aneurysm","volume":"143","author":"Oller","year":"2021","journal-title":"Circulation"},{"key":"bib36","doi-asserted-by":"publisher","first-page":"2611","DOI":"10.1161\/CIRCULATIONAHA.106.630400","article-title":"Thoracic aortic aneurysm and dissection: increasing prevalence and improved outcomes reported in a nationwide population-based study of more than 14,000 cases from 1987 to 2002","volume":"114","author":"Olsson","year":"2006","journal-title":"Circulation"},{"key":"bib37","doi-asserted-by":"publisher","first-page":"960","DOI":"10.1126\/science.aaw7894","article-title":"Organoids-on-a-chip","volume":"364","author":"Park","year":"2019","journal-title":"Science"},{"key":"bib38","doi-asserted-by":"publisher","first-page":"1351","DOI":"10.1161\/ATVBAHA.119.312787","article-title":"Role of vascular smooth muscle cell phenotypic switching and calcification in aortic aneurysm formation","volume":"39","author":"Petsophonsakul","year":"2019","journal-title":"Arteriosclerosis, Thrombosis, and Vascular Biology"},{"key":"bib39","doi-asserted-by":"publisher","first-page":"47","DOI":"10.14573\/altex.1811301","article-title":"3d human microvessel-on-a-chip model for studying monocyte-to-endothelium adhesion under flow - application in systems toxicology","volume":"1","author":"Poussin","year":"2020","journal-title":"Altex"},{"key":"bib40","doi-asserted-by":"publisher","first-page":"832","DOI":"10.1016\/j.jacc.2014.04.073","article-title":"A roadmap to investigate the genetic basis of bicuspid aortic valve and its complications: insights from the international BAVCon (Bicuspid aortic valve consortium)","volume":"64","author":"Prakash","year":"2014","journal-title":"Journal of the American College of Cardiology"},{"key":"bib41","doi-asserted-by":"publisher","DOI":"10.1002\/smll.201603737","article-title":"Biomechanical strain exacerbates inflammation on a progeria\u2010on\u2010a\u2010chip model","volume":"13","author":"Ribas","year":"2017","journal-title":"Small"},{"key":"bib42","doi-asserted-by":"publisher","first-page":"542","DOI":"10.1016\/j.redox.2013.10.011","article-title":"Mitochondrial fission induced by platelet-derived growth factor regulates vascular smooth muscle cell bioenergetics and cell proliferation","volume":"1","author":"Salabei","year":"2013","journal-title":"Redox Biology"},{"key":"bib43","doi-asserted-by":"publisher","first-page":"268","DOI":"10.1007\/s00380-012-0238-5","article-title":"Anti-correlation between longevity gene SirT1 and Notch signaling in ascending aorta biopsies from patients with bicuspid aortic valve disease","volume":"28","author":"Sciacca","year":"2013","journal-title":"Heart and Vessels"},{"key":"bib44","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-015-0295-3","article-title":"Notch1 signaling regulates the epithelial\u2013mesenchymal transition and invasion of breast cancer in a Slug-dependent manner","volume":"14","author":"Shao","year":"2015","journal-title":"Molecular Cancer"},{"key":"bib45","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.59961","article-title":"A lung-on-chip model of early Mycobacterium tuberculosis infection reveals an essential role for alveolar epithelial cells in controlling bacterial growth","volume":"9","author":"Thacker","year":"2020","journal-title":"eLife"},{"key":"bib46","doi-asserted-by":"publisher","first-page":"1776","DOI":"10.1093\/cvr\/cvy150","article-title":"Decreased mitochondrial respiration in aneurysmal aortas of Fibulin-4 mutant mice is linked to PGC1A regulation","volume":"114","author":"van der Pluijm","year":"2018","journal-title":"Cardiovascular Research"},{"key":"bib47","doi-asserted-by":"publisher","first-page":"509","DOI":"10.1113\/JP271301","article-title":"Mitochondrial dynamics, mitophagy and cardiovascular disease","volume":"594","author":"V\u00e1squez-Trincado","year":"2016","journal-title":"The Journal of Physiology"},{"key":"bib48","doi-asserted-by":"publisher","first-page":"1920","DOI":"10.1056\/NEJMra1207059","article-title":"Aortic dilatation in patients with bicuspid aortic valve","volume":"370","author":"Verma","year":"2014","journal-title":"New England Journal of Medicine"},{"key":"bib49","doi-asserted-by":"publisher","first-page":"2660","DOI":"10.1039\/C5LC00021A","article-title":"Artery-on-a-chip platform for automated, multimodal assessment of cerebral blood vessel structure and function","volume":"15","author":"Yasotharan","year":"2015","journal-title":"Lab on a Chip"},{"key":"bib50","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1038\/s41578-018-0034-7","article-title":"Advances in organ-on-a-chip engineering","volume":"3","author":"Zhang","year":"2018","journal-title":"Nature Reviews Materials"},{"key":"bib51","doi-asserted-by":"publisher","first-page":"913","DOI":"10.1016\/j.cell.2018.11.042","article-title":"A platform for generation of Chamber-Specific cardiac tissues and disease modeling","volume":"176","author":"Zhao","year":"2019","journal-title":"Cell"}],"container-title":["eLife"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/cdn.elifesciences.org\/articles\/69310\/elife-69310-v2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/cdn.elifesciences.org\/articles\/69310\/elife-69310-v2.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/elifesciences.org\/articles\/69310","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T21:18:22Z","timestamp":1697059102000},"score":1,"resource":{"primary":{"URL":"https:\/\/elifesciences.org\/articles\/69310"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9,6]]},"references-count":51,"alternative-id":["10.7554\/eLife.69310"],"URL":"https:\/\/doi.org\/10.7554\/elife.69310","archive":["CLOCKSS"],"relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2021.07.07.21260132","asserted-by":"subject"},{"id-type":"doi","id":"10.1101\/2021.07.07.21260132","asserted-by":"object"}],"is-supplemented-by":[{"id-type":"accession","id":"PXD026303","asserted-by":"subject"}],"has-review":[{"id-type":"doi","id":"10.7554\/eLife.69310.sa2","asserted-by":"object"},{"id-type":"doi","id":"10.7554\/eLife.69310.sa1","asserted-by":"object"}]},"ISSN":["2050-084X"],"issn-type":[{"value":"2050-084X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,9,6]]},"assertion":[{"value":"single anonymised","name":"peer_review_transparency","label":"Peer review transparency","group":{"name":"peer_review_taxonomy"}},{"value":"other reviewer(s), editor","name":"peer_review_interaction","label":"Peer review interaction","group":{"name":"peer_review_taxonomy"}},{"value":"review summaries, review reports, author\/editor communication, reviewer identities reviewer opt in, editor identities","name":"peer_review_published","label":"Peer review published","group":{"name":"peer_review_taxonomy"}},{"value":"open (sign in with ORCID iD required)","name":"post_publication_commenting","label":"Post publication commenting","group":{"name":"post_publication_commenting"}}],"article-number":"e69310"}}