{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T06:06:56Z","timestamp":1792390016448,"version":"4.4.0"},"reference-count":26,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2021,10,29]],"date-time":"2021-10-29T00:00:00Z","timestamp":1635465600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,10,29]],"date-time":"2021-10-29T00:00:00Z","timestamp":1635465600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,10,29]],"date-time":"2021-10-29T00:00:00Z","timestamp":1635465600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["5R01NS110552"],"award-info":[{"award-number":["5R01NS110552"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elifesciences.org"],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2021,10,7]]},"abstract":"<jats:p>\n                    Piezo1 is a mechanically activated ion channel involved in sensing forces in various cell types and tissues. Cryo-electron microscopy has revealed that the Piezo1 structure is bowl-shaped and capable of inducing membrane curvature via its extended footprint, which indirectly suggests that Piezo1 ion channels may bias each other\u2019s spatial distribution and interact functionally. Here, we use cell-attached patch-clamp electrophysiology and pressure-clamp stimulation to functionally examine large numbers of membrane patches from cells expressing Piezo1 endogenously at low levels and cells overexpressing Piezo1 at high levels. Our data, together with stochastic simulations of Piezo1 spatial distributions, show that both at endogenous densities (1\u20132 channels\/\u03bcm\n                    <jats:sup>2<\/jats:sup>\n                    ), and at non-physiological densities (10\u2013100 channels\/\u03bcm\n                    <jats:sup>2<\/jats:sup>\n                    ) predicted to cause substantial footprint overlap, Piezo1 density has no effect on its pressure sensitivity or open probability in the nominal absence of membrane tension. The results suggest that Piezo channels, at densities likely to be physiologically relevant, inherently behave as independent mechanotransducers. We propose that this property is essential for cells to transduce forces homogeneously across the entire cell membrane.\n                  <\/jats:p>","DOI":"10.7554\/elife.70988","type":"journal-article","created":{"date-parts":[[2021,10,29]],"date-time":"2021-10-29T03:00:14Z","timestamp":1635476414000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.70988","source":"Crossref","is-referenced-by-count":68,"title":["Piezo1 ion channels inherently function as independent mechanotransducers"],"prefix":"10.7554","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7316-9729","authenticated-orcid":true,"given":"Amanda H","family":"Lewis","sequence":"first","affiliation":[{"name":"Department of Neurobiology, Duke University Medical Center","place":["Durham, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7179-7609","authenticated-orcid":true,"given":"J\u00f6rg","family":"Grandl","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, Duke University Medical Center","place":["Durham, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4374","published-online":{"date-parts":[[2021,10,29]]},"reference":[{"key":"bib1","doi-asserted-by":"crossref","DOI":"10.1201\/b19708","volume-title":"Spatial Point Patterns: Methodology and Applications with R","author":"Baddeley","year":"2015"},{"key":"bib2","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1126\/science.1193270","article-title":"Piezo1 and Piezo2 are essential components of distinct mechanically activated cation channels","volume":"330","author":"Coste","year":"2010","journal-title":"Science"},{"key":"bib3","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms10366","article-title":"Removal of the mechanoprotective influence of the cytoskeleton reveals PIEZO1 is gated by bilayer tension","volume":"7","author":"Cox","year":"2016","journal-title":"Nature Communications"},{"key":"bib4","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-019-0514-3","article-title":"Myosin-II mediated traction forces evoke localized Piezo1-dependent Ca2+ flickers","volume":"2","author":"Ellefsen","year":"2019","journal-title":"Communications Biology"},{"key":"bib5","doi-asserted-by":"publisher","first-page":"438","DOI":"10.1016\/j.neuron.2020.02.010","article-title":"A Plug-and-Latch Mechanism for Gating the Mechanosensitive Piezo Channel","volume":"106","author":"Geng","year":"2020","journal-title":"Neuron"},{"key":"bib6","article-title":"PiezoClusteringModel","volume-title":"Software Heritage","author":"Grandl","year":"2021","unstructured":"Grandl J. 2021. 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