{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T14:04:11Z","timestamp":1787753051388,"version":"build-2784847793"},"reference-count":77,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2021,12,8]],"date-time":"2021-12-08T00:00:00Z","timestamp":1638921600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,12,8]],"date-time":"2021-12-08T00:00:00Z","timestamp":1638921600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,12,8]],"date-time":"2021-12-08T00:00:00Z","timestamp":1638921600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009455","name":"West Virginia University","doi-asserted-by":"publisher","award":["Bridge Funding"],"award-info":[{"award-number":["Bridge Funding"]}],"id":[{"id":"10.13039\/100009455","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["RO1EY028959"],"award-info":[{"award-number":["RO1EY028959"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elifesciences.org"],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2021,12,7]]},"abstract":"<jats:p>\n                    Despite mounting evidence that the mammalian retina is exceptionally reliant on proper NAD\n                    <jats:sup>+<\/jats:sup>\n                    homeostasis for health and function, the specific roles of subcellular NAD\n                    <jats:sup>+<\/jats:sup>\n                    pools in retinal development, maintenance, and disease remain obscure. Here, we show that deletion of the nuclear-localized NAD\n                    <jats:sup>+<\/jats:sup>\n                    synthase nicotinamide mononucleotide adenylyltransferase-1 (NMNAT1) in the developing murine retina causes early and severe degeneration of photoreceptors and select inner retinal neurons via multiple distinct cell death pathways. This severe phenotype is associated with disruptions to retinal central carbon metabolism, purine nucleotide synthesis, and amino acid pathways. Furthermore, transcriptomic and immunostaining approaches reveal dysregulation of a collection of photoreceptor and synapse-specific genes in NMNAT1 knockout retinas prior to detectable morphological or metabolic alterations. Collectively, our study reveals previously unrecognized complexity in NMNAT1-associated retinal degeneration and suggests a yet-undescribed role for NMNAT1 in gene regulation during photoreceptor terminal differentiation.\n                  <\/jats:p>","DOI":"10.7554\/elife.71185","type":"journal-article","created":{"date-parts":[[2021,12,8]],"date-time":"2021-12-08T08:01:27Z","timestamp":1638950487000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.71185","source":"Crossref","is-referenced-by-count":26,"title":["Nuclear NAD+-biosynthetic enzyme NMNAT1 facilitates development and early survival of retinal neurons"],"prefix":"10.7554","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6774-4133","authenticated-orcid":true,"given":"David","family":"Sokolov","sequence":"first","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences, Eye Institute, One Medical Center Drive, West Virginia University","place":["Morgantown, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily R","family":"Sechrest","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences, Eye Institute, One Medical Center Drive, West Virginia University","place":["Morgantown, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yekai","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences, Eye Institute, One Medical Center Drive, West Virginia University","place":["Morgantown, United States"]},{"name":"Department of Biochemistry, One Medical Center Drive, West Virginia University","place":["Morgantown, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Connor","family":"Nevin","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences, Eye Institute, One Medical Center Drive, West Virginia University","place":["Morgantown, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianhai","family":"Du","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences, Eye Institute, One Medical Center Drive, West Virginia University","place":["Morgantown, United States"]},{"name":"Department of Biochemistry, One Medical Center Drive, West Virginia University","place":["Morgantown, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8552-7850","authenticated-orcid":true,"given":"Saravanan","family":"Kolandaivelu","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences, Eye Institute, One Medical Center Drive, West Virginia University","place":["Morgantown, United States"]},{"name":"Department of Biochemistry, One Medical Center Drive, West Virginia University","place":["Morgantown, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4374","published-online":{"date-parts":[[2021,12,8]]},"reference":[{"key":"bib1","doi-asserted-by":"publisher","first-page":"859","DOI":"10.1038\/ncb2531","article-title":"Metabolic differentiation in the embryonic retina","volume":"14","author":"Agathocleous","year":"2012","journal-title":"Nature Cell Biology"},{"key":"bib2","doi-asserted-by":"publisher","first-page":"484","DOI":"10.1016\/j.tcb.2013.05.004","article-title":"Metabolism in physiological cell proliferation and differentiation","volume":"23","author":"Agathocleous","year":"2013","journal-title":"Trends in Cell Biology"},{"key":"bib3","doi-asserted-by":"publisher","first-page":"550","DOI":"10.1016\/j.neuron.2017.04.022","article-title":"The Dynamic Epigenetic Landscape of the Retina During Development, Reprogramming, and Tumorigenesis","volume":"94","author":"Aldiri","year":"2017","journal-title":"Neuron"},{"key":"bib4","doi-asserted-by":"publisher","first-page":"5225","DOI":"10.1167\/iovs.18-24708","article-title":"RDH12 Mutations Cause a Severe Retinal Degeneration With Relatively Spared Rod Function","volume":"59","author":"Aleman","year":"2018","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"bib5","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1016\/j.exer.2013.03.007","article-title":"The homeobox gene Otx2 in development and disease","volume":"111","author":"Beby","year":"2013","journal-title":"Experimental Eye Research"},{"key":"bib6","doi-asserted-by":"publisher","first-page":"2250","DOI":"10.1093\/hmg\/ddaa112","article-title":"An Alu-mediated duplication in NMNAT1, involved in NAD biosynthesis, causes a novel syndrome, SHILCA, affecting multiple tissues and organs","volume":"29","author":"Bedoni","year":"2020","journal-title":"Human Molecular Genetics"},{"key":"bib7","doi-asserted-by":"publisher","DOI":"10.1167\/tvst.9.5.14","article-title":"Disease Progression in Patients with Autosomal Dominant Retinitis Pigmentosa due to a Mutation in Inosine Monophosphate Dehydrogenase 1 (IMPDH1)","volume":"9","author":"Bennett","year":"2020","journal-title":"Translational Vision Science & Technology"},{"key":"bib8","first-page":"546","article-title":"Specific binding of peanut lectin to a class of retinal photoreceptor cells. A species comparison","volume":"25","author":"Blanks","year":"1984","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"bib9","doi-asserted-by":"publisher","first-page":"34","DOI":"10.1167\/iovs.05-0868","article-title":"Spectrum and frequency of mutations in IMPDH1 associated with autosomal dominant retinitis pigmentosa and leber congenital amaurosis","volume":"47","author":"Bowne","year":"2006","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"bib10","doi-asserted-by":"publisher","first-page":"3263","DOI":"10.1242\/dev.127043","article-title":"Photoreceptor cell fate specification in vertebrates","volume":"142","author":"Brzezinski","year":"2015","journal-title":"Development"},{"key":"bib11","doi-asserted-by":"publisher","first-page":"858","DOI":"10.1016\/j.tibs.2020.05.010","article-title":"Location, Location, Location: Compartmentalization of NAD+ Synthesis and Functions in Mammalian Cells","volume":"45","author":"Cambronne","year":"2020","journal-title":"Trends in Biochemical Sciences"},{"key":"bib12","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1016\/j.cmet.2015.05.023","article-title":"NAD(+) Metabolism and the Control of Energy Homeostasis: A Balancing Act between Mitochondria and the Nucleus","volume":"22","author":"Cant\u00f3","year":"2015","journal-title":"Cell Metabolism"},{"key":"bib13","doi-asserted-by":"publisher","first-page":"1412","DOI":"10.1016\/j.immuni.2019.04.005","article-title":"Cell Survival and Cytokine Release after Inflammasome Activation Is Regulated by the Toll-IL-1R Protein SARM","volume":"50","author":"Carty","year":"2019","journal-title":"Immunity"},{"key":"bib14","doi-asserted-by":"publisher","first-page":"615","DOI":"10.1038\/nrn3767","article-title":"Intrinsically different retinal progenitor cells produce specific types of progeny","volume":"15","author":"Cepko","year":"2014","journal-title":"Nature Reviews. Neuroscience"},{"key":"bib15","doi-asserted-by":"publisher","first-page":"972","DOI":"10.1038\/ng.2370","article-title":"Exome sequencing identifies NMNAT1 mutations as a cause of Leber congenital amaurosis","volume":"44","author":"Chiang","year":"2012","journal-title":"Nature Genetics"},{"key":"bib16","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.25946","article-title":"Glycolytic reliance promotes anabolism in photoreceptors","volume":"6","author":"Chinchore","year":"2017","journal-title":"eLife"},{"key":"bib17","doi-asserted-by":"publisher","first-page":"8862","DOI":"10.1073\/pnas.1103627108","article-title":"RAS-converting enzyme 1-mediated endoproteolysis is required for trafficking of rod phosphodiesterase 6 to photoreceptor outer segments","volume":"108","author":"Christiansen","year":"2011","journal-title":"PNAS"},{"key":"bib18","doi-asserted-by":"publisher","first-page":"2666","DOI":"10.1111\/j.1742-4658.2011.08193.x","article-title":"Reducing expression of NAD+ synthesizing enzyme NMNAT1 does not affect the rate of Wallerian degeneration","volume":"278","author":"Conforti","year":"2011","journal-title":"The FEBS Journal"},{"key":"bib19","doi-asserted-by":"publisher","first-page":"1188","DOI":"10.1002\/humu.22899","article-title":"Hidden Genetic Variation in LCA9-Associated Congenital Blindness Explained by 5\u2019UTR Mutations and Copy-Number Variations of NMNAT1","volume":"36","author":"Coppieters","year":"2015","journal-title":"Human Mutation"},{"key":"bib20","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.31437","article-title":"The formation of the light-sensing compartment of cone photoreceptors coincides with a transcriptional switch","volume":"6","author":"Daum","year":"2017","journal-title":"eLife"},{"key":"bib21","doi-asserted-by":"publisher","first-page":"1220","DOI":"10.1038\/sj.cdd.4401105","article-title":"Caspase-independent photoreceptor apoptosis in vivo and differential expression of apoptotic protease activating factor-1 and caspase-3 during retinal development","volume":"9","author":"Donovan","year":"2002","journal-title":"Cell Death and Differentiation"},{"key":"bib22","doi-asserted-by":"publisher","first-page":"154","DOI":"10.1016\/j.ydbio.2005.12.026","article-title":"Decreased expression of pro-apoptotic Bcl-2 family members during retinal development and differential sensitivity to cell death","volume":"291","author":"Donovan","year":"2006","journal-title":"Developmental Biology"},{"key":"bib23","doi-asserted-by":"publisher","first-page":"5723","DOI":"10.1523\/JNEUROSCI.23-13-05723.2003","article-title":"Caspase-independent photoreceptor apoptosis in mouse models of retinal degeneration","volume":"23","author":"Doonan","year":"2003","journal-title":"The Journal of Neuroscience"},{"key":"bib24","article-title":"Metabolite Profiles of Rod Photoreceptor Cell Death in Mouse Retinal Degeneration Models","volume":"55","author":"Du","year":"2014","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"bib25","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1016\/j.exer.2018.04.010","article-title":"NMNAT1 E257K variant, associated with Leber Congenital Amaurosis (LCA9), causes a mild retinal degeneration phenotype","volume":"173","author":"Eblimit","year":"2018","journal-title":"Experimental Eye Research"},{"key":"bib26","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1023\/A:1017585016010","article-title":"De Novo Biosynthesis of Purines in the Retina: Evolutionary Aspects","volume":"37","author":"Etingof","year":"2001","journal-title":"Journal of Evolutionary Biochemistry and Physiology"},{"key":"bib27","doi-asserted-by":"publisher","first-page":"1040","DOI":"10.1038\/ng.2361","article-title":"NMNAT1 mutations cause Leber congenital amaurosis","volume":"44","author":"Falk","year":"2012","journal-title":"Nature Genetics"},{"key":"bib28","doi-asserted-by":"publisher","first-page":"1118","DOI":"10.1016\/j.neuron.2021.02.009","article-title":"SARM1 is a metabolic sensor activated by an increased NMN\/NAD+ ratio to trigger axon degeneration","volume":"109","author":"Figley","year":"2021","journal-title":"Neuron"},{"key":"bib29","doi-asserted-by":"publisher","first-page":"130","DOI":"10.1002\/(SICI)1526-968X(200002)26:2<130::AID-GENE9>3.0.CO;2-I","article-title":"Retina- and ventral forebrain-specific Cre recombinase activity in transgenic mice","volume":"26","author":"Furuta","year":"2000","journal-title":"Genesis"},{"key":"bib30","doi-asserted-by":"publisher","first-page":"1925","DOI":"10.1016\/j.ajpath.2016.03.013","article-title":"Mouse Models of NMNAT1-Leber Congenital Amaurosis (LCA9) Recapitulate Key Features of the Human Disease","volume":"186","author":"Greenwald","year":"2016","journal-title":"The American Journal of Pathology"},{"key":"bib31","doi-asserted-by":"publisher","first-page":"644","DOI":"10.1093\/hmg\/ddab070","article-title":"Mutant Nmnat1 leads to a retina-specific decrease of NAD+ accompanied by increased poly(ADP-ribose) in a mouse model of NMNAT1-associated retinal degeneration","volume":"30","author":"Greenwald","year":"2021","journal-title":"Human Molecular Genetics"},{"key":"bib32","doi-asserted-by":"publisher","first-page":"3530","DOI":"10.1073\/pnas.1812941116","article-title":"Loss of MPC1 reprograms retinal metabolism to impair visual function","volume":"116","author":"Grenell","year":"2019","journal-title":"PNAS"},{"key":"bib33","doi-asserted-by":"publisher","first-page":"2156","DOI":"10.4049\/jimmunol.1601757","article-title":"MLKL Activation Triggers NLRP3-Mediated Processing and Release of IL-1\u03b2 Independently of Gasdermin-D","volume":"198","author":"Gutierrez","year":"2017","journal-title":"Journal of Immunology"},{"key":"bib34","doi-asserted-by":"publisher","first-page":"194","DOI":"10.1210\/er.2009-0026","article-title":"The secret life of NAD+: an old metabolite controlling new metabolic signaling pathways","volume":"31","author":"Houtkooper","year":"2010","journal-title":"Endocrine Reviews"},{"key":"bib35","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.aax4917","article-title":"IRF2 transcriptionally induces GSDMD expression for pyroptosis","volume":"12","author":"Kayagaki","year":"2019","journal-title":"Science Signaling"},{"key":"bib36","doi-asserted-by":"publisher","first-page":"357","DOI":"10.1038\/nmeth.3317","article-title":"HISAT: a fast spliced aligner with low memory requirements","volume":"12","author":"Kim","year":"2015","journal-title":"Nature Methods"},{"key":"bib37","doi-asserted-by":"publisher","first-page":"1035","DOI":"10.1038\/ng.2356","article-title":"Mutations in NMNAT1 cause Leber congenital amaurosis and identify a new disease pathway for retinal degeneration","volume":"44","author":"Koenekoop","year":"2012","journal-title":"Nature Genetics"},{"key":"bib38","doi-asserted-by":"publisher","first-page":"1147","DOI":"10.1136\/bjophthalmol-2016-309975","article-title":"Leber congenital amaurosis\/early-onset severe retinal dystrophy: clinical features, molecular genetics and therapeutic interventions","volume":"101","author":"Kumaran","year":"2017","journal-title":"The British Journal of Ophthalmology"},{"key":"bib39","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-018-0907-0","article-title":"Roles of Nmnat1 in the survival of retinal progenitors through the regulation of pro-apoptotic gene expression via histone acetylation","volume":"9","author":"Kuribayashi","year":"2018","journal-title":"Cell Death & Disease"},{"key":"bib40","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1016\/j.celrep.2016.08.073","article-title":"NAMPT-Mediated NAD(+) Biosynthesis Is Essential for Vision In Mice","volume":"17","author":"Lin","year":"2016","journal-title":"Cell Reports"},{"key":"bib41","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biology"},{"key":"bib42","doi-asserted-by":"publisher","first-page":"2116","DOI":"10.1177\/0271678X21992625","article-title":"Metabolomic and transcriptional profiling reveals bioenergetic stress and activation of cell death and inflammatory pathways in vivo after neuronal deletion of NAMPT","volume":"41","author":"Lundt","year":"2021","journal-title":"Journal of Cerebral Blood Flow and Metabolism"},{"key":"bib43","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1016\/j.tins.2019.11.005","article-title":"Fiery Cell Death: Pyroptosis in the Central Nervous System","volume":"43","author":"McKenzie","year":"2020","journal-title":"Trends in Neurosciences"},{"key":"bib44","doi-asserted-by":"publisher","first-page":"206","DOI":"10.1111\/j.1600-065X.2011.01044.x","article-title":"Caspase-1-Induced Pyroptotic Cell Death: Caspase-1-Induced Pyroptotic Cell Death","volume":"243","author":"Miao","year":"2011","journal-title":"Immunological Reviews"},{"key":"bib45","doi-asserted-by":"publisher","first-page":"4913","DOI":"10.4049\/jimmunol.1500953","article-title":"SARM1, Not MyD88, Mediates TLR7\/TLR9-Induced Apoptosis in Neurons","volume":"195","author":"Mukherjee","year":"2015","journal-title":"Journal of Immunology"},{"key":"bib46","doi-asserted-by":"publisher","first-page":"14598","DOI":"10.1073\/pnas.1206937109","article-title":"Receptor interacting protein kinase mediates necrotic cone but not rod cell death in a mouse model of inherited degeneration","volume":"109","author":"Murakami","year":"2012","journal-title":"PNAS"},{"key":"bib47","doi-asserted-by":"publisher","first-page":"428","DOI":"10.1038\/s41431-017-0029-7","article-title":"NMNAT1 variants cause cone and cone-rod dystrophy","volume":"26","author":"Nash","year":"2018","journal-title":"European Journal of Human Genetics"},{"key":"bib48","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-020-00354-w","article-title":"NAD+ metabolism, stemness, the immune response, and cancer","volume":"6","author":"Navas","year":"2021","journal-title":"Signal Transduction and Targeted Therapy"},{"key":"bib49","doi-asserted-by":"publisher","first-page":"367","DOI":"10.1111\/ceo.12462","article-title":"Cancer-like Metabolism of the Mammalian Retina: Mammalian Retina Metabolism","volume":"43","author":"Ng","year":"2015","journal-title":"Clinical & Experimental Ophthalmology"},{"key":"bib50","doi-asserted-by":"publisher","first-page":"284","DOI":"10.3109\/10409238.2015.1028612","article-title":"The human NAD metabolome: Functions, metabolism and compartmentalization","volume":"50","author":"Nikiforov","year":"2015","journal-title":"Critical Reviews in Biochemistry and Molecular Biology"},{"key":"bib51","doi-asserted-by":"publisher","DOI":"10.12688\/wellcomeopenres.14898.2","article-title":"Metabolic tracing reveals novel adaptations to skeletal muscle cell energy production pathways in response to NAD + depletion","volume":"3","author":"Oakey","year":"2018","journal-title":"Wellcome Open Research"},{"key":"bib52","doi-asserted-by":"publisher","first-page":"975","DOI":"10.1038\/ng.2357","article-title":"Mutations in NMNAT1 cause Leber congenital amaurosis with early-onset severe macular and optic atrophy","volume":"44","author":"Perrault","year":"2012","journal-title":"Nature Genetics"},{"key":"bib53","doi-asserted-by":"publisher","first-page":"290","DOI":"10.1038\/nbt.3122","article-title":"StringTie enables improved reconstruction of a transcriptome from RNA-seq reads","volume":"33","author":"Pertea","year":"2015","journal-title":"Nature Biotechnology"},{"key":"bib54","doi-asserted-by":"publisher","first-page":"1650","DOI":"10.1038\/nprot.2016.095","article-title":"Transcript-level expression analysis of RNA-seq experiments with HISAT, StringTie and Ballgown","volume":"11","author":"Pertea","year":"2016","journal-title":"Nature Protocols"},{"key":"bib55","doi-asserted-by":"publisher","first-page":"3677","DOI":"10.1242\/jcs.00735","article-title":"Phosphorylation of serine 10 in histone H3, what for?","volume":"116","author":"Prigent","year":"2003","journal-title":"Journal of Cell Science"},{"key":"bib56","doi-asserted-by":"publisher","DOI":"10.1186\/s13072-021-00384-w","article-title":"Epigenetic regulation of retinal development","volume":"14","author":"Raeisossadati","year":"2021","journal-title":"Epigenetics & Chromatin"},{"key":"bib57","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2012.165","article-title":"Dnmt1-dependent DNA methylation is essential for photoreceptor terminal differentiation and retinal neuron survival","volume":"3","author":"Rhee","year":"2012","journal-title":"Cell Death & Disease"},{"key":"bib58","first-page":"1455","article-title":"Embryonic markers of cone differentiation","volume":"22","author":"Rodgers","year":"2016","journal-title":"Molecular Vision"},{"key":"bib59","doi-asserted-by":"publisher","DOI":"10.1126\/science.aan5780","article-title":"Metabolic regulation of transcription through compartmentalized NAD+ biosynthesis","volume":"360","author":"Ryu","year":"2018","journal-title":"Science"},{"key":"bib60","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.13663","article-title":"Disruption of glycolytic flux is a signal for inflammasome signaling and pyroptotic cell death","volume":"5","author":"Sanman","year":"2016","journal-title":"eLife"},{"key":"bib61","doi-asserted-by":"publisher","first-page":"17228","DOI":"10.1074\/jbc.M115.637850","article-title":"Characterization of Leber Congenital Amaurosis-associated NMNAT1 Mutants","volume":"290","author":"Sasaki","year":"2015","journal-title":"The Journal of Biological Chemistry"},{"key":"bib62","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2020.113252","article-title":"cADPR is a gene dosage-sensitive biomarker of SARM1 activity in healthy, compromised, and degenerating axons","volume":"329","author":"Sasaki","year":"2020","journal-title":"Experimental Neurology"},{"key":"bib63","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.62027","article-title":"SARM1 depletion rescues NMNAT1-dependent photoreceptor cell death and retinal degeneration","volume":"9","author":"Sasaki","year":"2020","journal-title":"eLife"},{"key":"bib64","doi-asserted-by":"publisher","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: an open-source platform for biological-image analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nature Methods"},{"key":"bib65","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1006987","article-title":"Tet-mediated DNA hydroxymethylation regulates retinal neurogenesis by modulating cell-extrinsic signaling pathways","volume":"13","author":"Seritrakul","year":"2017","journal-title":"PLOS Genetics"},{"key":"bib66","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2018956118","article-title":"Absence of retbindin blocks glycolytic flux, disrupts metabolic homeostasis, and leads to photoreceptor degeneration","volume":"118","author":"Sinha","year":"2021","journal-title":"PNAS"},{"key":"bib67","doi-asserted-by":"publisher","first-page":"19149","DOI":"10.1074\/jbc.M311058200","article-title":"Phosducin Facilitates Light-driven Transducin Translocation in Rod Photoreceptors","volume":"279","author":"Sokolov","year":"2004","journal-title":"Journal of Biological Chemistry"},{"key":"bib68","doi-asserted-by":"publisher","first-page":"20908","DOI":"10.1074\/jbc.M113.470302","article-title":"The NAD+ synthesis enzyme nicotinamide mononucleotide adenylyltransferase (NMNAT1) regulates ribosomal RNA transcription","volume":"288","author":"Song","year":"2013","journal-title":"The Journal of Biological Chemistry"},{"key":"bib69","doi-asserted-by":"publisher","first-page":"477","DOI":"10.1038\/s41577-019-0165-0","article-title":"The NLRP3 inflammasome: molecular activation and regulation to therapeutics","volume":"19","author":"Swanson","year":"2019","journal-title":"Nature Reviews. Immunology"},{"key":"bib70","doi-asserted-by":"publisher","first-page":"563","DOI":"10.1038\/nrn2880","article-title":"Transcriptional regulation of photoreceptor development and homeostasis in the mammalian retina","volume":"11","author":"Swaroop","year":"2010","journal-title":"Nature Reviews. Neuroscience"},{"key":"bib71","doi-asserted-by":"publisher","first-page":"700","DOI":"10.1038\/nrm2970","article-title":"Molecular mechanisms of necroptosis: an ordered cellular explosion","volume":"11","author":"Vandenabeele","year":"2010","journal-title":"Nature Reviews. Molecular Cell Biology"},{"key":"bib72","doi-asserted-by":"publisher","DOI":"10.1101\/210757","article-title":"Targeted Deletion of Nmnat1 in Mouse Retina Leads to Early Severe Retinal Dystrophy","volume-title":"bioRxiv","author":"Wang","year":"2017"},{"key":"bib73","doi-asserted-by":"publisher","first-page":"756","DOI":"10.1126\/science.aal0092","article-title":"Vitamin B3 modulates mitochondrial vulnerability and prevents glaucoma in aged mice","volume":"355","author":"Williams","year":"2017","journal-title":"Science"},{"key":"bib74","doi-asserted-by":"publisher","first-page":"10278","DOI":"10.1074\/jbc.RA119.007983","article-title":"Proline mediates metabolic communication between retinal pigment epithelial cells and the retina","volume":"294","author":"Yam","year":"2019","journal-title":"The Journal of Biological Chemistry"},{"key":"bib75","doi-asserted-by":"publisher","first-page":"12405","DOI":"10.1074\/jbc.M111.304469","article-title":"Regulation of poly(ADP-ribose) polymerase-1-dependent gene expression through promoter-directed recruitment of a nuclear NAD+ synthase","volume":"287","author":"Zhang","year":"2012","journal-title":"The Journal of Biological Chemistry"},{"key":"bib76","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-020-03103-7","article-title":"Selective knockdown of hexokinase 2 in rods leads to age-related photoreceptor degeneration and retinal metabolic remodeling","volume":"11","author":"Zhang","year":"2020","journal-title":"Cell Death & Disease"},{"key":"bib77","doi-asserted-by":"publisher","first-page":"452","DOI":"10.1016\/j.isci.2019.05.001","article-title":"A Cell-Permeant Mimetic of NMN Activates SARM1 to Produce Cyclic ADP-Ribose and Induce Non-apoptotic Cell Death","volume":"15","author":"Zhao","year":"2019","journal-title":"IScience"}],"container-title":["eLife"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/cdn.elifesciences.org\/articles\/71185\/elife-71185-v2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/cdn.elifesciences.org\/articles\/71185\/elife-71185-v2.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/elifesciences.org\/articles\/71185","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T21:21:01Z","timestamp":1697059261000},"score":1,"resource":{"primary":{"URL":"https:\/\/elifesciences.org\/articles\/71185"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,12,8]]},"references-count":77,"alternative-id":["10.7554\/eLife.71185"],"URL":"https:\/\/doi.org\/10.7554\/elife.71185","archive":["CLOCKSS"],"relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2021.05.05.442836","asserted-by":"subject"},{"id-type":"doi","id":"10.1101\/2021.05.05.442836","asserted-by":"object"}],"is-supplemented-by":[{"id-type":"accession","id":"GSE178312","asserted-by":"subject"}],"has-review":[{"id-type":"doi","id":"10.7554\/eLife.71185.sa0","asserted-by":"object"},{"id-type":"doi","id":"10.7554\/eLife.71185.sa1","asserted-by":"object"},{"id-type":"doi","id":"10.7554\/eLife.71185.sa2","asserted-by":"object"}]},"ISSN":["2050-084X"],"issn-type":[{"value":"2050-084X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,12,8]]},"assertion":[{"value":"single anonymised","name":"peer_review_transparency","label":"Peer review transparency","group":{"name":"peer_review_taxonomy"}},{"value":"other reviewer(s), editor","name":"peer_review_interaction","label":"Peer review interaction","group":{"name":"peer_review_taxonomy"}},{"value":"review summaries, review reports, author\/editor communication, reviewer identities reviewer opt in, editor identities","name":"peer_review_published","label":"Peer review published","group":{"name":"peer_review_taxonomy"}},{"value":"open (sign in with ORCID iD required)","name":"post_publication_commenting","label":"Post publication commenting","group":{"name":"post_publication_commenting"}}],"article-number":"e71185"}}