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Since mTEC numbers are limited, their function is complemented by thymic dendritic cells (DCs), which transfer mTEC-produced self-antigens via cooperative antigen transfer (CAT). While CAT is required for effective T cell selection, many aspects remain enigmatic. Given the recently described heterogeneity of mTECs and DCs, it is unclear whether the antigen acquisition from a particular TEC subset is mediated by preferential pairing with a specific subset of DCs. Using several relevant\n                    <jats:italic>Cre<\/jats:italic>\n                    -based mouse models that control for the expression of fluorescent proteins, we have found that, in regards to CAT, each subset of thymic DCs preferentially targets a distinct mTEC subset(s). Importantly, XCR1\n                    <jats:sup>+<\/jats:sup>\n                    -activated DC subset represented the most potent subset in CAT. Interestingly, thymic DCs can also acquire antigens from more than one mTEC, and of these, monocyte-derived dendritic cells (moDCs) were determined to be the most efficient. moDCs also represented the most potent DC subset in the acquisition of antigen from other DCs. These findings suggest a preferential pairing model for the distribution of mTEC-derived antigens among distinct populations of thymic DCs.\n                  <\/jats:p>","DOI":"10.7554\/elife.71578","type":"journal-article","created":{"date-parts":[[2022,1,12]],"date-time":"2022-01-12T09:00:49Z","timestamp":1641978049000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.71578","source":"Crossref","is-referenced-by-count":28,"title":["A model of preferential pairing between epithelial and dendritic cells in thymic antigen transfer"],"prefix":"10.7554","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3672-7629","authenticated-orcid":true,"given":"Matou\u0161","family":"Vobo\u0159il","sequence":"first","affiliation":[{"name":"Laboratory of Immunobiology, Institute of Molecular Genetics of the Czech Academy of Sciences","place":["Prague, Czech 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