{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T23:21:53Z","timestamp":1782861713437,"version":"3.54.5"},"reference-count":63,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2021,10,25]],"date-time":"2021-10-25T00:00:00Z","timestamp":1635120000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,10,25]],"date-time":"2021-10-25T00:00:00Z","timestamp":1635120000000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,10,25]],"date-time":"2021-10-25T00:00:00Z","timestamp":1635120000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["Grant-in-Aid for Early-Career Scientists 19K16700"],"award-info":[{"award-number":["Grant-in-Aid for Early-Career Scientists 19K16700"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elifesciences.org"],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2021,10,21]]},"abstract":"<jats:p>\n                    Antigens (Ags) with multivalent and repetitive structure elicit IgG production in a T-cell-independent manner. However, the mechanisms by which such T-cell-independent type-2 (TI-2) Ags induce IgG responses remain obscure. Here, we report that B-cell receptor (BCR) engagement with a TI-2 Ag but not with a T-cell-dependent (TD) Ag was able to induce the transcription of\n                    <jats:italic>Aicda<\/jats:italic>\n                    encoding activation-induced cytidine deaminase (AID) and efficient class switching to IgG3 upon costimulation with IL-1 or IFN-\u03b1 in mouse B cells. TI-2 Ags strongly induced the phosphorylation of protein kinase C (PKC)\u03b4 and PKC\u03b4 mediated the\n                    <jats:italic>Aicda<\/jats:italic>\n                    transcription through the induction of BATF, the key transcriptional regulator of\n                    <jats:italic>Aicda<\/jats:italic>\n                    . In PKC\u03b4-deficient mice, production of IgG was intact against TD Ag but abrogated against typical TI-2 Ags as well as commensal bacteria, and experimental disruption of the gut epithelial barrier resulted in fatal bacteremia. Thus, our results have revealed novel molecular requirements for class switching in the TI-2 response and highlighted its importance in homeostatic commensal-specific IgG production.\n                  <\/jats:p>","DOI":"10.7554\/elife.72116","type":"journal-article","created":{"date-parts":[[2021,10,25]],"date-time":"2021-10-25T08:00:42Z","timestamp":1635148842000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.72116","source":"Crossref","is-referenced-by-count":7,"title":["Protein kinase C\u03b4 is essential for the IgG response against T-cell-independent type 2 antigens and commensal bacteria"],"prefix":"10.7554","volume":"10","author":[{"given":"Saori","family":"Fukao","sequence":"first","affiliation":[{"name":"Division of Cancer Cell Biology, Research Institute for Biomedical Sciences (RIBS), Tokyo University of Science","place":["Noda, 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