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Whereas hundreds of genes are known to be essential for male germline proliferation and differentiation, the contribution of several genes remains uncharacterized. The predominant expression of the latest globin family member, androglobin (Adgb), in mammalian testis tissue prompted us to assess its physiological function in spermatogenesis. Adgb knockout mice display male infertility, reduced testis weight, impaired maturation of elongating spermatids, abnormal sperm shape, and ultrastructural defects in microtubule and mitochondrial organization. Epididymal sperm from Adgb knockout animals display multiple flagellar malformations including coiled, bifid or shortened flagella, and erratic acrosomal development. Following immunoprecipitation and mass spectrometry, we could identify septin 10 (Sept10) as interactor of Adgb. The Sept10-Adgb interaction was confirmed both\n                    <jats:italic>in vivo<\/jats:italic>\n                    using testis lysates and\n                    <jats:italic>in vitro<\/jats:italic>\n                    by reciprocal co-immunoprecipitation experiments. Furthermore, the absence of Adgb leads to mislocalization of Sept10 in sperm, indicating defective manchette and sperm annulus formation. Finally,\n                    <jats:italic>in vitro<\/jats:italic>\n                    data suggest that Adgb contributes to Sept10 proteolysis in a calmodulin-dependent manner. Collectively, our results provide evidence that Adgb is essential for murine spermatogenesis and further suggest that Adgb is required for sperm head shaping via the manchette and proper flagellum formation.\n                  <\/jats:p>","DOI":"10.7554\/elife.72374","type":"journal-article","created":{"date-parts":[[2022,6,14]],"date-time":"2022-06-14T06:15:23Z","timestamp":1655187323000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.72374","source":"Crossref","is-referenced-by-count":33,"title":["Androglobin, a chimeric mammalian globin, is required for male fertility"],"prefix":"10.7554","volume":"11","author":[{"given":"Anna","family":"Keppner","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/022fs9h90","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Endocrinology, Metabolism and Cardiovascular system, University of Fribourg","place":["Fribourg, 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