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The\n                    <jats:italic>Arabidopsis<\/jats:italic>\n                    YTH domain protein ECT2 is thought to bind to mRNA at URU(m\n                    <jats:sup>6<\/jats:sup>\n                    A)Y sites, yet RR(m\n                    <jats:sup>6<\/jats:sup>\n                    A)CH is the canonical m\n                    <jats:sup>6<\/jats:sup>\n                    A consensus site in all eukaryotes and ECT2 functions require m\n                    <jats:sup>6<\/jats:sup>\n                    A-binding activity. Here, we apply iCLIP (\n                    <jats:italic>i<\/jats:italic>\n                    ndividual nucleotide resolution\n                    <jats:italic>c<\/jats:italic>\n                    ross\n                    <jats:italic>l<\/jats:italic>\n                    inking and\n                    <jats:italic>i<\/jats:italic>\n                    mmuno\n                    <jats:italic>p<\/jats:italic>\n                    recipitation) and HyperTRIBE (\n                    <jats:italic>t<\/jats:italic>\n                    argets of\n                    <jats:italic>R<\/jats:italic>\n                    NA-binding proteins\n                    <jats:italic>i<\/jats:italic>\n                    dentified\n                    <jats:italic>b<\/jats:italic>\n                    y\n                    <jats:italic>e<\/jats:italic>\n                    diting) to define high-quality target sets of ECT2 and analyze the patterns of enriched sequence motifs around ECT2 crosslink sites. Our analyses show that ECT2 does in fact bind to RR(m\n                    <jats:sup>6<\/jats:sup>\n                    A)CH. Pyrimidine-rich motifs are enriched around, but not at m\n                    <jats:sup>6<\/jats:sup>\n                    A sites, reflecting a preference for\n                    <jats:italic>N6<\/jats:italic>\n                    -adenosine methylation of RRACH\/GGAU islands in pyrimidine-rich regions. Such motifs, particularly oligo-U and UNUNU upstream of m\n                    <jats:sup>6<\/jats:sup>\n                    A sites, are also implicated in ECT2 binding via its intrinsically disordered region (IDR). Finally, URUAY-type motifs are enriched at ECT2 crosslink sites, but their distinct properties suggest function as sites of competition between binding of ECT2 and as yet unidentified RNA-binding proteins. Our study provides coherence between genetic and molecular studies of m\n                    <jats:sup>6<\/jats:sup>\n                    A-YTH function in plants and reveals new insight into the mode of RNA recognition by YTH domain-containing proteins.\n                  <\/jats:p>","DOI":"10.7554\/elife.72375","type":"journal-article","created":{"date-parts":[[2021,9,30]],"date-time":"2021-09-30T12:01:08Z","timestamp":1633003268000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.72375","source":"Crossref","is-referenced-by-count":78,"title":["Principles of mRNA targeting via the Arabidopsis m6A-binding protein ECT2"],"prefix":"10.7554","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0605-0407","authenticated-orcid":true,"given":"Laura","family":"Arribas-Hern\u00e1ndez","sequence":"first","affiliation":[{"name":"University of Copenhagen, Copenhagen Plant Science Center","place":["Copenhagen N, Denmark"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Rennie","sequence":"first","affiliation":[{"name":"Department of Biology, University of Copenhagen","place":["Copenhagen, Denmark"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tino","family":"K\u00f6ster","sequence":"additional","affiliation":[{"name":"University of Bielefeld, Faculty of Biology, RNA Biology and Molecular Physiology","place":["Bielefeld, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4675-4898","authenticated-orcid":true,"given":"Carlotta","family":"Porcelli","sequence":"additional","affiliation":[{"name":"University of Copenhagen, Copenhagen Plant Science Center","place":["Copenhagen N, Denmark"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Lewinski","sequence":"additional","affiliation":[{"name":"University of Bielefeld, Faculty of Biology, RNA Biology and Molecular Physiology","place":["Bielefeld, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1341-1381","authenticated-orcid":true,"given":"Dorothee","family":"Staiger","sequence":"additional","affiliation":[{"name":"University of Bielefeld, Faculty of Biology, RNA Biology and Molecular Physiology","place":["Bielefeld, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1516-879X","authenticated-orcid":true,"given":"Robin","family":"Andersson","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Copenhagen","place":["Copenhagen, Denmark"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1083-1150","authenticated-orcid":true,"given":"Peter","family":"Brodersen","sequence":"additional","affiliation":[{"name":"University of Copenhagen, Copenhagen Plant Science Center","place":["Copenhagen N, Denmark"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4374","published-online":{"date-parts":[[2021,9,30]]},"reference":[{"key":"bib1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1002732","article-title":"RNA methylation by the MIS complex regulates a cell fate decision in yeast","volume":"8","author":"Agarwala","year":"2012","journal-title":"PLOS Genetics"},{"key":"bib2","doi-asserted-by":"publisher","DOI":"10.1038\/npre.2012.6837.1","article-title":"Detecting differential usage of exons from RNA-seq data","volume":"1","author":"Anders","year":"2012","journal-title":"Nature Precedings"},{"key":"bib3","doi-asserted-by":"publisher","first-page":"1146","DOI":"10.1016\/j.celrep.2018.10.020","article-title":"N6-Methyladenosine Inhibits Local Ribonucleolytic Cleavage to Stabilize mRNAs in Arabidopsis","volume":"25","author":"Anderson","year":"2018","journal-title":"Cell 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