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During the development of the nematode\n                    <jats:italic>Caenorhabditis elegans<\/jats:italic>\n                    , many somatic and germ cells undergo apoptosis. These cells are engulfed and degraded by their neighboring cells. We discovered a novel role of autophagosomes in facilitating the degradation of apoptotic cells using a real-time imaging technique. Specifically, the double-membrane autophagosomes in engulfing cells are recruited to the surfaces of phagosomes containing apoptotic cells and subsequently fuse to phagosomes, allowing the inner vesicle to enter the phagosomal lumen. Mutants defective in the production of autophagosomes display significant defects in the degradation of apoptotic cells, demonstrating the importance of autophagosomes to this process. The signaling pathway led by the phagocytic receptor CED-1, the adaptor protein CED-6, and the large GTPase dynamin (DYN-1) promotes the recruitment of autophagosomes to phagosomes. Moreover, the subsequent fusion of autophagosomes with phagosomes requires the functions of the small GTPase RAB-7 and the HOPS complex. Further observations suggest that autophagosomes provide apoptotic cell-degradation activities in addition to and in parallel of lysosomes. Our findings reveal that, unlike the single-membrane,\n                    <jats:italic>L<\/jats:italic>\n                    C3-\n                    <jats:italic>a<\/jats:italic>\n                    ssociated\n                    <jats:underline>p<\/jats:underline>\n                    hagocytosis (LAP) vesicles reported to facilitate phagocytosis in mammals, it is the canonical double-membrane autophagosomes that facilitate the clearance of\n                    <jats:italic>C. elegans<\/jats:italic>\n                    apoptotic cells. These findings add autophagosomes to the collection of intracellular organelles that contribute to phagosome maturation, identify novel crosstalk between the autophagy and phagosome maturation pathways, and discover the upstream signaling molecules that initiate this crosstalk.\n                  <\/jats:p>","DOI":"10.7554\/elife.72466","type":"journal-article","created":{"date-parts":[[2022,1,4]],"date-time":"2022-01-04T08:01:13Z","timestamp":1641283273000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.72466","source":"Crossref","is-referenced-by-count":30,"title":["Autophagosomes fuse to phagosomes and facilitate the degradation of apoptotic cells in Caenorhabditis elegans"],"prefix":"10.7554","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1231-2602","authenticated-orcid":true,"given":"Omar","family":"Pe\u00f1a-Ramos","sequence":"first","affiliation":[{"name":"Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine","place":["Houston, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucia","family":"Chiao","sequence":"additional","affiliation":[{"name":"Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine","place":["Houston, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianghua","family":"Liu","sequence":"additional","affiliation":[{"name":"Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine","place":["Houston, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaomeng","family":"Yu","sequence":"additional","affiliation":[{"name":"Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine","place":["Houston, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianyou","family":"Yao","sequence":"additional","affiliation":[{"name":"Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine","place":["Houston, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henry","family":"He","sequence":"additional","affiliation":[{"name":"Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine","place":["Houston, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2585-0418","authenticated-orcid":true,"given":"Zheng","family":"Zhou","sequence":"additional","affiliation":[{"name":"Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine","place":["Houston, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4374","published-online":{"date-parts":[[2022,1,4]]},"reference":[{"key":"bib1","doi-asserted-by":"publisher","first-page":"1307","DOI":"10.1242\/jcs.107805","article-title":"CORVET and HOPS tethering complexes - coordinators of endosome and lysosome fusion","volume":"126","author":"Balderhaar","year":"2013","journal-title":"Journal of Cell Science"},{"key":"bib2","doi-asserted-by":"publisher","first-page":"3414","DOI":"10.1073\/pnas.94.7.3414","article-title":"The Caenorhabditis elegans gene unc-76 and its human homologs define a new gene family involved in axonal outgrowth and fasciculation","volume":"94","author":"Bloom","year":"1997","journal-title":"PNAS"},{"key":"bib3","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2121-11-40","article-title":"Roles of CUP-5, the Caenorhabditis elegans orthologue of human TRPML1, in lysosome and gut granule biogenesis","volume":"11","author":"Campbell","year":"2010","journal-title":"BMC Cell Biology"},{"key":"bib4","doi-asserted-by":"publisher","first-page":"2022","DOI":"10.4161\/auto.26323","article-title":"Autophagy genes coordinate with the class II PI\/PtdIns 3-kinase PIKI-1 to regulate apoptotic cell clearance in C. elegans","volume":"9","author":"Cheng","year":"2013","journal-title":"Autophagy"},{"key":"bib5","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1895\/wormbook.1.89.1","article-title":"Genetic balancers","author":"Edgley","year":"2006","journal-title":"WormBook\u202f: The Online Review of C. 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