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excessive amounts. Heavy-metal transporting P-type (P\n                    <jats:sub>IB<\/jats:sub>\n                    ) ATPases are crucial for homeostasis, conferring cellular detoxification and redistribution through transport of these ions across cellular membranes. No structural information is available for the P\n                    <jats:sub>IB-4<\/jats:sub>\n                    -ATPases, the subclass with the broadest cargo scope, and hence even their topology remains elusive. Here, we present structures and complementary functional analyses of an archetypal P\n                    <jats:sub>IB-4<\/jats:sub>\n                    -ATPase, sCoaT from\n                    <jats:italic>Sulfitobacter<\/jats:italic>\n                    sp. NAS14-1. The data disclose the architecture, devoid of classical so-called heavy-metal-binding domains (HMBDs), and provide fundamentally new insights into the mechanism and diversity of heavy-metal transporters. We reveal several novel P-type ATPase features, including a dual role in heavy-metal release and as an internal counter ion of an invariant histidine. We also establish that the turnover of P\n                    <jats:sub>IB<\/jats:sub>\n                    -ATPases is potassium independent, contrasting to many other P-type ATPases. Combined with new inhibitory compounds, our results open up for efforts in for example drug discovery, since P\n                    <jats:sub>IB-4<\/jats:sub>\n                    -ATPases function as virulence factors in many pathogens.\n                  <\/jats:p>","DOI":"10.7554\/elife.73124","type":"journal-article","created":{"date-parts":[[2021,12,24]],"date-time":"2021-12-24T12:00:10Z","timestamp":1640347210000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.73124","source":"Crossref","is-referenced-by-count":15,"title":["Structure and ion-release mechanism of PIB-4-type ATPases"],"prefix":"10.7554","volume":"10","author":[{"given":"Christina","family":"Gr\u00f8nberg","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/035b05819","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biomedical Sciences, University of Copenhagen","place":["Copenhagen, 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