{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T10:11:01Z","timestamp":1790676661988,"version":"4.1.0"},"reference-count":69,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2022,5,19]],"date-time":"2022-05-19T00:00:00Z","timestamp":1652918400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2022,5,19]],"date-time":"2022-05-19T00:00:00Z","timestamp":1652918400000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2022,5,19]],"date-time":"2022-05-19T00:00:00Z","timestamp":1652918400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007904","name":"Texas A and M University","doi-asserted-by":"publisher","award":["Clinical Science Translational Research Institute Pilot Grant CSTR2016-1"],"award-info":[{"award-number":["Clinical Science Translational Research Institute Pilot Grant CSTR2016-1"]}],"id":[{"id":"10.13039\/100007904","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000185","name":"Defense Advanced Research Projects Agency","doi-asserted-by":"publisher","award":["HR001118A0025-FoF-FP-006"],"award-info":[{"award-number":["HR001118A0025-FoF-FP-006"]}],"id":[{"id":"10.13039\/100000185","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R21AI139738-01A1"],"award-info":[{"award-number":["R21AI139738-01A1"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["1R01AI141607-01A1"],"award-info":[{"award-number":["1R01AI141607-01A1"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["1R21GM132705-01"],"award-info":[{"award-number":["1R21GM132705-01"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008982","name":"National Science Foundation","doi-asserted-by":"publisher","award":["DBI1532188"],"award-info":[{"award-number":["DBI1532188"]}],"id":[{"id":"10.13039\/501100008982","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008982","name":"National Science Foundation","doi-asserted-by":"publisher","award":["NSF0854684"],"award-info":[{"award-number":["NSF0854684"]}],"id":[{"id":"10.13039\/501100008982","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81371773"],"award-info":[{"award-number":["81371773"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["RHD084339"],"award-info":[{"award-number":["RHD084339"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elifesciences.org"],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2022,4,26]]},"abstract":"<jats:p>\n                    The phagocytosis and destruction of pathogens in lysosomes constitute central elements of innate immune defense. Here, we show that\n                    <jats:italic>Brucella<\/jats:italic>\n                    , the causative agent of brucellosis, the most prevalent bacterial zoonosis globally, subverts this immune defense pathway by activating regulated IRE1\u03b1-dependent decay (RIDD) of\n                    <jats:italic>Bloc1s1<\/jats:italic>\n                    mRNA encoding BLOS1, a protein that promotes endosome\u2013lysosome fusion. RIDD-deficient cells and mice harboring a RIDD-incompetent variant of IRE1\u03b1 were resistant to infection. Inactivation of the\n                    <jats:italic>Bloc1s1<\/jats:italic>\n                    gene impaired the ability to assemble BLOC-1-related complex (BORC), resulting in differential recruitment of BORC-related lysosome trafficking components, perinuclear trafficking of\n                    <jats:italic>Brucella<\/jats:italic>\n                    -containing vacuoles (BCVs), and enhanced susceptibility to infection. The RIDD-resistant\n                    <jats:italic>Bloc1s1<\/jats:italic>\n                    variant maintains the integrity of BORC and a higher-level association of BORC-related components that promote centrifugal lysosome trafficking, resulting in enhanced BCV peripheral trafficking and lysosomal destruction, and resistance to infection. These findings demonstrate that host RIDD activity on BLOS1 regulates\n                    <jats:italic>Brucella<\/jats:italic>\n                    intracellular parasitism by disrupting BORC-directed lysosomal trafficking. Notably, coronavirus murine hepatitis virus also subverted the RIDD\u2013BLOS1 axis to promote intracellular replication. Our work establishes BLOS1 as a novel immune defense factor whose activity is hijacked by diverse pathogens.\n                  <\/jats:p>","DOI":"10.7554\/elife.73625","type":"journal-article","created":{"date-parts":[[2022,4,26]],"date-time":"2022-04-26T12:15:33Z","timestamp":1650975333000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.73625","source":"Crossref","is-referenced-by-count":21,"title":["Brucella activates the host RIDD pathway to subvert BLOS1-directed immune defense"],"prefix":"10.7554","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1657-5189","authenticated-orcid":true,"given":"Kelsey Michelle","family":"Wells","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01tx6pn92","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbial Pathogenesis and Immunology, College of Medicine, Texas A&M Health Science Center","place":["Bryan, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5805-3354","authenticated-orcid":true,"given":"Kai","family":"He","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01f5ytq51","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Electrical and Computer Engineering, Texas A&M University","place":["College Station, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aseem","family":"Pandey","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01tx6pn92","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbial Pathogenesis and Immunology, College of Medicine, Texas A&M Health Science Center","place":["Bryan, United States"]},{"id":[{"id":"https:\/\/ror.org\/01f5ytq51","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Veterinary Pathobiology, Texas A&M University","place":["College Station, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Cabello","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01tx6pn92","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbial Pathogenesis and Immunology, College of Medicine, Texas A&M Health Science Center","place":["Bryan, United States"]},{"id":[{"id":"https:\/\/ror.org\/01f5ytq51","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Veterinary Pathobiology, Texas A&M University","place":["College Station, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongmei","family":"Zhang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01tx6pn92","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbial Pathogenesis and Immunology, College of Medicine, Texas A&M Health Science Center","place":["Bryan, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Yang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01tx6pn92","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbial Pathogenesis and Immunology, College of Medicine, Texas A&M Health Science Center","place":["Bryan, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel","family":"Gomez","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01f5ytq51","id-type":"ROR","asserted-by":"publisher"}],"name":"Texas A&M Veterinary Medical Diagnostic Laboratory, Texas A&M University","place":["College Station, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6213-5248","authenticated-orcid":true,"given":"Yue","family":"Liu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00js3aw79","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Plant Sciences, Key Laboratory of Zoonosis Research, Ministry of Education, Jilin University","place":["Jilin, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haowu","family":"Chang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00js3aw79","id-type":"ROR","asserted-by":"publisher"}],"name":"Key Laboratory of Symbolic Computation and Knowledge Engineering, Ministry of Education, College of Computer Science and Technology, Jilin University","place":["Changchun, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueqiang","family":"Li","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00js3aw79","id-type":"ROR","asserted-by":"publisher"}],"name":"Key Laboratory of Symbolic Computation and Knowledge Engineering, Ministry of Education, College of Computer Science and Technology, Jilin University","place":["Changchun, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2058-7123","authenticated-orcid":true,"given":"Hao","family":"Zhang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00js3aw79","id-type":"ROR","asserted-by":"publisher"}],"name":"Key Laboratory of Symbolic Computation and Knowledge Engineering, Ministry of Education, College of Computer Science and Technology, Jilin University","place":["Changchun, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuehuang","family":"Feng","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01tx6pn92","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbial Pathogenesis and Immunology, College of Medicine, Texas A&M Health Science Center","place":["Bryan, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciana Fachini","family":"da Costa","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01f5ytq51","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Veterinary Pathobiology, Texas A&M University","place":["College Station, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9876-3385","authenticated-orcid":true,"given":"Richard","family":"Metz","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01f5ytq51","id-type":"ROR","asserted-by":"publisher"}],"name":"Genomics and Bioinformatics Services, Texas A&M University","place":["College Station, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3892-7082","authenticated-orcid":true,"given":"Charles D","family":"Johnson","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01f5ytq51","id-type":"ROR","asserted-by":"publisher"}],"name":"Genomics and Bioinformatics Services, Texas A&M University","place":["College Station, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cameron Lee","family":"Martin","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01f5ytq51","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Poultry Science, Texas A&M University","place":["College Station, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jill","family":"Skrobarczyk","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01f5ytq51","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Poultry Science, Texas A&M University","place":["College Station, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luc R","family":"Berghman","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01f5ytq51","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Poultry Science, Texas A&M University","place":["College Station, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2442-4679","authenticated-orcid":true,"given":"Kristin L","family":"Patrick","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01tx6pn92","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbial Pathogenesis and Immunology, College of Medicine, Texas A&M Health Science Center","place":["Bryan, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3485-3506","authenticated-orcid":true,"given":"Julian","family":"Leibowitz","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01tx6pn92","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbial Pathogenesis and Immunology, College of Medicine, Texas A&M Health Science Center","place":["Bryan, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allison","family":"Ficht","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01f5ytq51","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Molecular and Cellular Medicine, College of Medicine, Texas A&M Health Science Center","place":["College Station, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sing-Hoi","family":"Sze","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01f5ytq51","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Computer Science and Engineering, Dwight Look College of Engineering, Texas A&M University","place":["College Station, United States"]},{"id":[{"id":"https:\/\/ror.org\/01f5ytq51","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biochemistry & Biophysics, Texas A&M University","place":["College Station, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianxun","family":"Song","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01tx6pn92","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbial Pathogenesis and Immunology, College of Medicine, Texas A&M Health Science Center","place":["Bryan, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4347-2476","authenticated-orcid":true,"given":"Xiaoning","family":"Qian","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01f5ytq51","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Electrical and Computer Engineering, Texas A&M University","place":["College Station, United States"]},{"id":[{"id":"https:\/\/ror.org\/01f5ytq51","id-type":"ROR","asserted-by":"publisher"}],"name":"TEES-AgriLife Center for Bioinformatics & Genomic Systems Engineering, Texas A&M University","place":["College Station, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1723-6867","authenticated-orcid":true,"given":"Qing-Ming","family":"Qin","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01tx6pn92","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbial Pathogenesis and Immunology, College of Medicine, Texas A&M Health Science Center","place":["Bryan, United States"]},{"id":[{"id":"https:\/\/ror.org\/00js3aw79","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Plant Sciences, Key Laboratory of Zoonosis Research, Ministry of Education, Jilin University","place":["Jilin, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas A","family":"Ficht","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01f5ytq51","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Veterinary Pathobiology, Texas A&M University","place":["College Station, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3878-5064","authenticated-orcid":true,"given":"Paul","family":"de Figueiredo","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01tx6pn92","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbial Pathogenesis and Immunology, College of Medicine, Texas A&M Health Science Center","place":["Bryan, United States"]},{"id":[{"id":"https:\/\/ror.org\/01f5ytq51","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Veterinary Pathobiology, Texas A&M University","place":["College Station, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4374","published-online":{"date-parts":[[2022,5,19]]},"reference":[{"key":"bib1","doi-asserted-by":"publisher","DOI":"10.1038\/npre.2010.4282.2","article-title":"Differential expression analysis for sequence count data","volume":"1","author":"Anders","year":"2010","journal-title":"Nature Precedings"},{"key":"bib2","doi-asserted-by":"publisher","first-page":"1118","DOI":"10.1083\/jcb.201809027","article-title":"Degradation of Blos1 mRNA by IRE1 repositions lysosomes and protects cells from stress","volume":"218","author":"Bae","year":"2019","journal-title":"The Journal of Cell Biology"},{"key":"bib3","doi-asserted-by":"publisher","first-page":"4492","DOI":"10.1128\/JVI.00017-08","article-title":"Coronavirus infection modulates the unfolded protein response and mediates sustained translational repression","volume":"82","author":"Bechill","year":"2008","journal-title":"Journal of Virology"},{"key":"bib4","doi-asserted-by":"publisher","first-page":"2186","DOI":"10.1128\/MCB.00013-15","article-title":"Cleavage of BLOC1S1 mRNA by IRE1 Is Sequence Specific, Temporally Separate from XBP1 Splicing, and Dispensable for Cell Viability under Acute Endoplasmic Reticulum Stress","volume":"35","author":"Bright","year":"2015","journal-title":"Molecular and Cellular Biology"},{"key":"bib5","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0006529","article-title":"A versatile viral system for expression and depletion of proteins in mammalian cells","volume":"4","author":"Campeau","year":"2009","journal-title":"PLOS ONE"},{"key":"bib6","doi-asserted-by":"publisher","first-page":"340","DOI":"10.1038\/nature10348","article-title":"Ebola virus entry requires the cholesterol transporter Niemann-Pick C1","volume":"477","author":"Carette","year":"2011","journal-title":"Nature"},{"key":"bib7","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003402","article-title":"Tripping on acid: trans-kingdom perspectives on biological acids in immunity and pathogenesis","volume":"9","author":"Criscitiello","year":"2013","journal-title":"PLOS Pathogens"},{"key":"bib8","doi-asserted-by":"publisher","first-page":"E869","DOI":"10.1073\/pnas.1115623109","article-title":"The molecular basis for selective inhibition of unconventional mRNA splicing by an IRE1-binding small molecule","volume":"109","author":"Cross","year":"2012","journal-title":"PNAS"},{"key":"bib9","doi-asserted-by":"publisher","first-page":"1044","DOI":"10.1111\/j.1462-5822.2011.01601.x","article-title":"Identification of a Brucella spp. secreted effector specifically interacting with human small GTPase Rab2","volume":"13","author":"de Barsy","year":"2011","journal-title":"Cellular Microbiology"},{"key":"bib10","doi-asserted-by":"publisher","first-page":"1505","DOI":"10.1016\/j.ajpath.2015.03.003","article-title":"Pathogenesis and immunobiology of brucellosis: review of Brucella-host interactions","volume":"185","author":"de Figueiredo","year":"2015","journal-title":"The American Journal of Pathology"},{"key":"bib11","doi-asserted-by":"publisher","first-page":"1378","DOI":"10.1111\/j.1365-2958.2008.06487.x","article-title":"Identification of VceA and VceC, two members of the VjbR regulon that are translocated into macrophages by the Brucella type IV secretion system","volume":"70","author":"de Jong","year":"2008","journal-title":"Molecular Microbiology"},{"key":"bib12","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00418-12","article-title":"Sensing of bacterial type IV secretion via the unfolded protein response","volume":"4","author":"de Jong","year":"2013","journal-title":"MBio"},{"key":"bib13","doi-asserted-by":"publisher","first-page":"443","DOI":"10.1073\/pnas.221575398","article-title":"The genome sequence of the facultative intracellular pathogen Brucella melitensis","volume":"99","author":"DelVecchio","year":"2002","journal-title":"PNAS"},{"key":"bib14","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2021.102192","article-title":"Interactions between fungal hyaluronic acid and host CD44 promote internalization by recruiting host autophagy proteins to forming phagosomes","volume":"24","author":"Ding","year":"2021","journal-title":"IScience"},{"key":"bib15","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1093\/bioinformatics\/bts635","article-title":"STAR: ultrafast universal RNA-seq aligner","volume":"29","author":"Dobin","year":"2013","journal-title":"Bioinformatics (Oxford, England)"},{"key":"bib16","doi-asserted-by":"publisher","first-page":"396","DOI":"10.1111\/cmi.12224","article-title":"Identification of a type IV secretion substrate of Brucella abortus that participates in the early stages of intracellular survival","volume":"16","author":"D\u00f6hmer","year":"2014","journal-title":"Cellular Microbiology"},{"key":"bib17","doi-asserted-by":"crossref","DOI":"10.1016\/B978-0-12-369408-9.00042-1","volume-title":"Brucellosis. In: Vaccines for Biodefense and Emerging and Neglected Diseases","author":"Ficht","year":"2009"},{"key":"bib18","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a013169","article-title":"Endoplasmic reticulum stress sensing in the unfolded protein response","volume":"5","author":"Gardner","year":"2013","journal-title":"Cold Spring Harbor Perspectives in Biology"},{"key":"bib19","doi-asserted-by":"publisher","first-page":"449","DOI":"10.1093\/bib\/bbr042","article-title":"Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium","volume":"12","author":"Gaudet","year":"2011","journal-title":"Briefings in Bioinformatics"},{"key":"bib20","doi-asserted-by":"publisher","first-page":"461","DOI":"10.1016\/s0378-1135(02)00230-4","article-title":"How to substantiate eradication of bovine brucellosis when aspecific serological reactions occur in the course of brucellosis testing","volume":"90","author":"Godfroid","year":"2002","journal-title":"Veterinary Microbiology"},{"key":"bib21","doi-asserted-by":"publisher","first-page":"1950","DOI":"10.1016\/j.celrep.2016.10.062","article-title":"BORC Functions Upstream of Kinesins 1 and 3 to Coordinate Regional Movement of Lysosomes along Different Microtubule Tracks","volume":"17","author":"Guardia","year":"2016","journal-title":"Cell Reports"},{"key":"bib22","doi-asserted-by":"publisher","first-page":"562","DOI":"10.1016\/j.cell.2009.07.017","article-title":"IRE1alpha kinase activation modes control alternate endoribonuclease outputs to determine divergent cell fates","volume":"138","author":"Han","year":"2009","journal-title":"Cell"},{"key":"bib23","doi-asserted-by":"publisher","first-page":"153","DOI":"10.4049\/jimmunol.1901511","article-title":"TRIM14 Is a Key Regulator of the Type I IFN Response during Mycobacterium tuberculosis Infection","volume":"205","author":"Hoffpauir","year":"2020","journal-title":"Journal of Immunology (Baltimore, Md)"},{"key":"bib24","doi-asserted-by":"publisher","first-page":"104","DOI":"10.1126\/science.1129631","article-title":"Decay of endoplasmic reticulum-localized mRNAs during the unfolded protein response","volume":"313","author":"Hollien","year":"2006","journal-title":"Science (New York, N.Y.)"},{"key":"bib25","doi-asserted-by":"publisher","first-page":"323","DOI":"10.1083\/jcb.200903014","article-title":"Regulated Ire1-dependent decay of messenger RNAs in mammalian cells","volume":"186","author":"Hollien","year":"2009","journal-title":"The Journal of Cell Biology"},{"key":"bib26","doi-asserted-by":"publisher","first-page":"632","DOI":"10.4049\/jimmunol.1801550","article-title":"Brucella Infection Regulates Thioredoxin-Interacting Protein Expression to Facilitate Intracellular Survival by Reducing the Production of Nitric Oxide and Reactive Oxygen Species","volume":"204","author":"Hu","year":"2020","journal-title":"Journal of Immunology (Baltimore, Md)"},{"key":"bib27","doi-asserted-by":"publisher","first-page":"307","DOI":"10.1084\/jem.20111298","article-title":"IRE1\u03b1 activation protects mice against acetaminophen-induced hepatotoxicity","volume":"209","author":"Hur","year":"2012","journal-title":"The Journal of Experimental Medicine"},{"key":"bib28","doi-asserted-by":"publisher","first-page":"16657","DOI":"10.1073\/pnas.0903775106","article-title":"Function of IRE1 alpha in the placenta is essential for placental development and embryonic viability","volume":"106","author":"Iwawaki","year":"2009","journal-title":"PNAS"},{"key":"bib29","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1083\/jcb.201210038","article-title":"The BLOC-1 complex promotes endosomal maturation by recruiting the Rab5 GTPase-activating protein Msb3","volume":"201","author":"John Peter","year":"2013","journal-title":"The Journal of Cell Biology"},{"key":"bib30","doi-asserted-by":"publisher","first-page":"453","DOI":"10.1038\/ncb2204","article-title":"Lysosomal positioning coordinates cellular nutrient responses","volume":"13","author":"Korolchuk","year":"2011","journal-title":"Nature Cell Biology"},{"key":"bib31","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00361-18","article-title":"Caspase-1 and Caspase-11 Mediate Pyroptosis, Inflammation, and Control of Brucella Joint Infection","volume":"86","author":"Lacey","year":"2018","journal-title":"Infection and Immunity"},{"key":"bib32","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1016\/j.cell.2007.10.057","article-title":"Structure of the dual enzyme Ire1 reveals the basis for catalysis and regulation in nonconventional RNA splicing","volume":"132","author":"Lee","year":"2008","journal-title":"Cell"},{"key":"bib33","doi-asserted-by":"publisher","first-page":"250","DOI":"10.1016\/j.cmet.2012.07.007","article-title":"IRE1\u03b1 induces thioredoxin-interacting protein to activate the NLRP3 inflammasome and promote programmed cell death under irremediable ER stress","volume":"16","author":"Lerner","year":"2012","journal-title":"Cell Metabolism"},{"key":"bib34","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-12-323","article-title":"RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome","volume":"12","author":"Li","year":"2011","journal-title":"BMC Bioinformatics"},{"key":"bib35","doi-asserted-by":"publisher","first-page":"1261","DOI":"10.1111\/j.1462-5822.2011.01618.x","article-title":"In search of Brucella abortus type IV secretion substrates: screening and identification of four proteins translocated into host cells through VirB system","volume":"13","author":"Marchesini","year":"2011","journal-title":"Cellular Microbiology"},{"key":"bib36","doi-asserted-by":"publisher","first-page":"411","DOI":"10.1038\/ni.1857","article-title":"TLR activation of the transcription factor XBP1 regulates innate immune responses in macrophages","volume":"11","author":"Martinon","year":"2010","journal-title":"Nature Immunology"},{"key":"bib37","doi-asserted-by":"publisher","first-page":"4288","DOI":"10.1093\/nar\/gks042","article-title":"Differential expression analysis of multifactor RNA-Seq experiments with respect to biological variation","volume":"40","author":"McCarthy","year":"2012","journal-title":"Nucleic Acids Research"},{"key":"bib38","doi-asserted-by":"publisher","first-page":"D204","DOI":"10.1093\/nar\/gkp1019","article-title":"PANTHER version 7: improved phylogenetic trees, orthologs and collaboration with the Gene Ontology Consortium","volume":"38","author":"Mi","year":"2010","journal-title":"Nucleic Acids Research"},{"key":"bib39","doi-asserted-by":"publisher","first-page":"317","DOI":"10.1016\/j.chom.2017.07.017","article-title":"A Brucella Type IV Effector Targets the COG Tethering Complex to Remodel Host Secretory Traffic and Promote Intracellular Replication","volume":"22","author":"Miller","year":"2017","journal-title":"Cell Host & Microbe"},{"key":"bib40","doi-asserted-by":"publisher","first-page":"2873","DOI":"10.1091\/mbc.E15-02-0074","article-title":"Ire1-mediated decay in mammalian cells relies on mRNA sequence, structure, and translational status","volume":"26","author":"Moore","year":"2015","journal-title":"Molecular Biology of the Cell"},{"key":"bib41","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003556","article-title":"Brucella modulates secretory trafficking via multiple type IV secretion effector proteins","volume":"9","author":"Myeni","year":"2013","journal-title":"PLOS Pathogens"},{"key":"bib42","doi-asserted-by":"publisher","DOI":"10.3390\/cells10020300","article-title":"Mechanisms of Coronavirus Nsp1-Mediated Control of Host and Viral Gene Expression","volume":"10","author":"Nakagawa","year":"2021","journal-title":"Cells"},{"key":"bib43","doi-asserted-by":"publisher","first-page":"637","DOI":"10.1016\/j.chom.2017.04.008","article-title":"Global Reprogramming of Host Kinase Signaling in Response to Fungal Infection","volume":"21","author":"Pandey","year":"2017","journal-title":"Cell Host & Microbe"},{"key":"bib44","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2018.00103","article-title":"Activation of Host IRE1\u03b1-Dependent Signaling Axis Contributes the Intracellular Parasitism of Brucella melitensis","volume":"8","author":"Pandey","year":"2018","journal-title":"Frontiers in Cellular and Infection Microbiology"},{"key":"bib45","doi-asserted-by":"publisher","first-page":"2959","DOI":"10.1016\/j.jprot.2011.07.020","article-title":"Comparative proteome analysis of Brucella abortus 2308 and its virB type IV secretion system mutant reveals new T4SS-related candidate proteins","volume":"74","author":"Paredes-Cervantes","year":"2011","journal-title":"Journal of Proteomics"},{"key":"bib46","doi-asserted-by":"publisher","first-page":"5711","DOI":"10.1128\/IAI.66.12.5711-5724.1998","article-title":"Brucella abortus transits through the autophagic pathway and replicates in the endoplasmic reticulum of nonprofessional phagocytes","volume":"66","author":"Pizarro-Cerd\u00e1","year":"1998","journal-title":"Infection and Immunity"},{"key":"bib47","doi-asserted-by":"publisher","first-page":"176","DOI":"10.1016\/j.devcel.2015.02.011","article-title":"BORC, a multisubunit complex that regulates lysosome positioning","volume":"33","author":"Pu","year":"2015","journal-title":"Developmental Cell"},{"key":"bib48","doi-asserted-by":"publisher","first-page":"4183","DOI":"10.1083\/jcb.201703094","article-title":"A Ragulator-BORC interaction controls lysosome positioning in response to amino acid availability","volume":"216","author":"Pu","year":"2017","journal-title":"The Journal of Cell Biology"},{"key":"bib49","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000110","article-title":"RNAi screen of endoplasmic reticulum-associated host factors reveals a role for IRE1alpha in supporting Brucella replication","volume":"4","author":"Qin","year":"2008","journal-title":"PLOS Pathogens"},{"key":"bib50","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002078","article-title":"Functional analysis of host factors that mediate the intracellular lifestyle of Cryptococcus neoformans","volume":"7","author":"Qin","year":"2011","journal-title":"PLOS Pathogens"},{"key":"bib51","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1093\/bioinformatics\/btp616","article-title":"edgeR: a Bioconductor package for differential expression analysis of digital gene expression data","volume":"26","author":"Robinson","year":"2010","journal-title":"Bioinformatics (Oxford, England)"},{"key":"bib52","doi-asserted-by":"publisher","first-page":"519","DOI":"10.1038\/nrm2199","article-title":"Signal integration in the endoplasmic reticulum unfolded protein response","volume":"8","author":"Ron","year":"2007","journal-title":"Nature Reviews. Molecular Cell Biology"},{"key":"bib53","doi-asserted-by":"publisher","first-page":"130","DOI":"10.1016\/j.vetmic.2012.03.029","article-title":"The virB-encoded type IV secretion system is critical for establishment of infection and persistence of Brucella ovis infection in mice","volume":"159","author":"S\u00e1","year":"2012","journal-title":"Veterinary Microbiology"},{"key":"bib54","doi-asserted-by":"publisher","first-page":"346","DOI":"10.1016\/j.tcb.2018.01.007","article-title":"GCN5L1\/BLOS1 Links Acetylation, Organelle Remodeling, and Metabolism","volume":"28","author":"Scott","year":"2018","journal-title":"Trends in Cell Biology"},{"key":"bib55","doi-asserted-by":"publisher","first-page":"1041","DOI":"10.1016\/j.chembiol.2012.07.007","article-title":"Identification of the binding site of Brucella VirB8 interaction inhibitors","volume":"19","author":"Smith","year":"2012","journal-title":"Chemistry & Biology"},{"key":"bib56","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003785","article-title":"Brucella induces an unfolded protein response via TcpB that supports intracellular replication in macrophages","volume":"9","author":"Smith","year":"2013","journal-title":"PLOS Pathogens"},{"key":"bib57","doi-asserted-by":"publisher","first-page":"487","DOI":"10.1016\/j.cmet.2012.09.004","article-title":"Silencing of lipid metabolism genes through IRE1\u03b1-mediated mRNA decay lowers plasma lipids in mice","volume":"16","author":"So","year":"2012","journal-title":"Cell Metabolism"},{"key":"bib58","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1016\/j.chom.2011.12.002","article-title":"Selective subversion of autophagy complexes facilitates completion of the Brucella intracellular cycle","volume":"11","author":"Starr","year":"2012","journal-title":"Cell Host & Microbe"},{"key":"bib59","doi-asserted-by":"publisher","DOI":"10.1002\/cpbi.5","article-title":"The GeneCards Suite: From Gene Data Mining to Disease Genome Sequence Analyses","volume":"54","author":"Stelzer","year":"2016","journal-title":"Current Protocols in Bioinformatics"},{"key":"bib60","article-title":"ICE Analysis tool, v.3.0","author":"Synthego Performance Analysis","year":"2019","unstructured":"Synthego Performance Analysis. 2019. ICE Analysis tool, v.3.0. https:\/\/www.synthego.com\/product\/bioinfomatics\/crispr-analysis [Accessed July 7, 2018]."},{"key":"bib61","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004747","article-title":"Yip1A, a novel host factor for the activation of the IRE1 pathway of the unfolded protein response during Brucella infection","volume":"11","author":"Taguchi","year":"2015","journal-title":"PLOS Pathogens"},{"key":"bib62","doi-asserted-by":"publisher","first-page":"1249","DOI":"10.1126\/science.abc8665","article-title":"Structural basis for translational shutdown and immune evasion by the Nsp1 protein of SARS-CoV-2","volume":"369","author":"Thoms","year":"2020","journal-title":"Science (New York, N.Y.)"},{"key":"bib63","doi-asserted-by":"publisher","first-page":"1081","DOI":"10.1126\/science.1209038","article-title":"The unfolded protein response: from stress pathway to homeostatic regulation","volume":"334","author":"Walter","year":"2011","journal-title":"Science (New York, N.Y.)"},{"key":"bib64","doi-asserted-by":"publisher","first-page":"W77","DOI":"10.1093\/nar\/gkt439","article-title":"WEB-based GEne SeT AnaLysis Toolkit (WebGestalt): update 2013","volume":"41","author":"Wang","year":"2013","journal-title":"Nucleic Acids Research"},{"key":"bib65","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2016.00019","article-title":"Brucella suis Vaccine Strain 2 Induces Endoplasmic Reticulum Stress that Affects Intracellular Replication in Goat Trophoblast Cells In vitro","volume":"6","author":"Wang","year":"2016","journal-title":"Frontiers in Cellular and Infection Microbiology"},{"key":"bib66","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.221036","article-title":"GCN5L1 interacts with \u03b1TAT1 and RanBP2 to regulate hepatic \u03b1-tubulin acetylation and lysosome trafficking","volume":"131","author":"Wu","year":"2018","journal-title":"Journal of Cell Science"},{"key":"bib67","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagrm.2020.194598","article-title":"The emerging roles of GCN5L1 in mitochondrial and vacuolar organelle biology","volume":"1864","author":"Wu","year":"2021","journal-title":"Biochimica et Biophysica Acta. Gene Regulatory Mechanisms"},{"key":"bib68","doi-asserted-by":"publisher","first-page":"3707","DOI":"10.1080\/15548627.2021.1894759","article-title":"BLOC1S1\/GCN5L1\/BORCS1 is a critical mediator for the initiation of autolysosomal tubulation","volume":"17","author":"Wu","year":"2021","journal-title":"Autophagy"},{"key":"bib69","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.00548","article-title":"Transcriptomic and Metabolomics Profiling of PHAGE-Host Interactions between PHAGE PaP1 and Pseudomonas aeruginosa","volume":"8","author":"Zhao","year":"2017","journal-title":"Frontiers in Microbiology"}],"container-title":["eLife"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/cdn.elifesciences.org\/articles\/73625\/elife-73625-v1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/cdn.elifesciences.org\/articles\/73625\/elife-73625-v1.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/elifesciences.org\/articles\/73625","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T21:09:09Z","timestamp":1697058549000},"score":1,"resource":{"primary":{"URL":"https:\/\/elifesciences.org\/articles\/73625"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,19]]},"references-count":69,"alternative-id":["10.7554\/eLife.73625"],"URL":"https:\/\/doi.org\/10.7554\/elife.73625","archive":["CLOCKSS"],"relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2021.11.04.467275","asserted-by":"subject"},{"id-type":"doi","id":"10.1101\/2021.11.04.467275","asserted-by":"object"}],"has-review":[{"id-type":"doi","id":"10.7554\/eLife.73625.sa2","asserted-by":"object"},{"id-type":"doi","id":"10.7554\/eLife.73625.sa0","asserted-by":"object"},{"id-type":"doi","id":"10.7554\/eLife.73625.sa1","asserted-by":"object"}]},"ISSN":["2050-084X"],"issn-type":[{"value":"2050-084X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,5,19]]},"assertion":[{"value":"single anonymised","name":"peer_review_transparency","label":"Peer review transparency","group":{"name":"peer_review_taxonomy"}},{"value":"other reviewer(s), editor","name":"peer_review_interaction","label":"Peer review interaction","group":{"name":"peer_review_taxonomy"}},{"value":"review summaries, review reports, author\/editor communication, reviewer identities reviewer opt in, editor identities","name":"peer_review_published","label":"Peer review published","group":{"name":"peer_review_taxonomy"}},{"value":"open (sign in with ORCID iD required)","name":"post_publication_commenting","label":"Post publication commenting","group":{"name":"post_publication_commenting"}}],"article-number":"e73625"}}