{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T15:58:26Z","timestamp":1792166306475,"version":"4.4.0"},"reference-count":69,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2022,5,10]],"date-time":"2022-05-10T00:00:00Z","timestamp":1652140800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2022,5,10]],"date-time":"2022-05-10T00:00:00Z","timestamp":1652140800000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2022,5,10]],"date-time":"2022-05-10T00:00:00Z","timestamp":1652140800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003000","name":"Stichting MS Research","doi-asserted-by":"publisher","award":["20-1087"],"award-info":[{"award-number":["20-1087"]}],"id":[{"id":"10.13039\/501100003000","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elifesciences.org"],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2022,4,29]]},"abstract":"<jats:p>\n                    Multiple sclerosis (MS) is a chronic demyelinating disease characterised by immune cell infiltration resulting in lesions that preferentially affect periventricular areas of the brain. Despite research efforts to define the role of various immune cells in MS pathogenesis, the focus has been on a few immune cell populations while full-spectrum analysis, encompassing others such as natural killer (NK) cells, has not been performed. Here, we used single-cell mass cytometry (CyTOF) to profile the immune landscape of brain periventricular areas \u2013 septum and choroid plexus \u2013 and of the circulation from donors with MS, dementia and controls without neurological disease. Using a 37-marker panel, we revealed the infiltration of T cells and antibody-secreting cells in periventricular brain regions and identified a novel NK cell signature specific to MS. CD56\n                    <jats:sup>bright<\/jats:sup>\n                    NK cells were accumulated in the septum of MS donors and displayed an activated and migratory phenotype, similar to that of CD56\n                    <jats:sup>bright<\/jats:sup>\n                    NK cells in the circulation. We validated this signature by multiplex immunohistochemistry and found that the number of NK cells with high expression of granzyme K, typical of the CD56\n                    <jats:sup>bright<\/jats:sup>\n                    subset, was increased in both periventricular lesions and the choroid plexus of donors with MS. Together, our multi-tissue single-cell data shows that CD56\n                    <jats:sup>bright<\/jats:sup>\n                    NK cells accumulate in the periventricular brain regions of MS patients, bringing NK cells back to the spotlight of MS pathology.\n                  <\/jats:p>","DOI":"10.7554\/elife.73849","type":"journal-article","created":{"date-parts":[[2022,5,3]],"date-time":"2022-05-03T08:15:20Z","timestamp":1651565720000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.73849","source":"Crossref","is-referenced-by-count":42,"title":["Single-cell profiling reveals periventricular CD56bright NK cell accumulation in multiple sclerosis"],"prefix":"10.7554","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5956-4519","authenticated-orcid":true,"given":"Sabela","family":"Rodr\u00edguez-Lorenzo","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/008xxew50","id-type":"ROR","asserted-by":"publisher"}],"name":"MS Center Amsterdam, Department of Molecular Cell Biology and Immunology, Amsterdam Neuroscience, Vrije Universiteit Amsterdam, Amsterdam UMC","place":["Amsterdam, Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7569-0470","authenticated-orcid":true,"given":"Lynn","family":"van Olst","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/008xxew50","id-type":"ROR","asserted-by":"publisher"}],"name":"MS Center Amsterdam, Department of Molecular Cell Biology and Immunology, Amsterdam Neuroscience, Vrije Universiteit Amsterdam, Amsterdam UMC","place":["Amsterdam, Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7849-9302","authenticated-orcid":true,"given":"Carla","family":"Rodriguez-Mogeda","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/008xxew50","id-type":"ROR","asserted-by":"publisher"}],"name":"MS Center Amsterdam, Department of Molecular Cell Biology and 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Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ernesto","family":"Rodr\u00edguez","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/008xxew50","id-type":"ROR","asserted-by":"publisher"}],"name":"MS Center Amsterdam, Department of Molecular Cell Biology and Immunology, Amsterdam Neuroscience, Vrije Universiteit Amsterdam, Amsterdam UMC","place":["Amsterdam, Netherlands"]},{"id":[{"id":"https:\/\/ror.org\/008xxew50","id-type":"ROR","asserted-by":"publisher"}],"name":"Amsterdam UMC, Vrije Universiteit Amsterdam, Department of Molecular Cell Biology and Immunology, Cancer Center Amsterdam, Amsterdam Infection and Immunity Institute","place":["Amsterdam, Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9488-2918","authenticated-orcid":true,"given":"Gijs","family":"Kooij","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/008xxew50","id-type":"ROR","asserted-by":"publisher"}],"name":"MS Center Amsterdam, Department of Molecular Cell Biology and Immunology, Amsterdam Neuroscience, Vrije Universiteit Amsterdam, Amsterdam UMC","place":["Amsterdam, Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7904-7124","authenticated-orcid":true,"given":"Helga E","family":"de Vries","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/008xxew50","id-type":"ROR","asserted-by":"publisher"}],"name":"MS Center Amsterdam, Department of Molecular Cell Biology and Immunology, Amsterdam Neuroscience, Vrije Universiteit Amsterdam, Amsterdam UMC","place":["Amsterdam, 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