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Whereas AIRE-expressing (Aire\n                    <jats:sup>+<\/jats:sup>\n                    ) mTECs undergo constant turnover in the adult thymus, mechanisms underlying differentiation of postnatal mTECs remain to be discovered. Integrative analysis of single-cell assays for transposase-accessible chromatin (scATAC-seq) and single-cell RNA sequencing (scRNA-seq) suggested the presence of proliferating mTECs with a specific chromatin structure, which express high levels of Aire and co-stimulatory molecules, CD80 (Aire\n                    <jats:sup>+<\/jats:sup>\n                    CD80\n                    <jats:sup>hi<\/jats:sup>\n                    ). Proliferating Aire\n                    <jats:sup>+<\/jats:sup>\n                    CD80\n                    <jats:sup>hi<\/jats:sup>\n                    mTECs detected using Fucci technology express a minimal number of Aire-dependent TSAs and are converted into quiescent Aire\n                    <jats:sup>+<\/jats:sup>\n                    CD80\n                    <jats:sup>hi<\/jats:sup>\n                    mTECs expressing high levels of TSAs after a transit amplification. These data provide evidence for the existence of transit-amplifying Aire\n                    <jats:sup>+<\/jats:sup>\n                    mTEC precursors during the Aire\n                    <jats:sup>+<\/jats:sup>\n                    mTEC differentiation process of the postnatal thymus.\n                  <\/jats:p>","DOI":"10.7554\/elife.73998","type":"journal-article","created":{"date-parts":[[2022,4,22]],"date-time":"2022-04-22T04:15:14Z","timestamp":1650600914000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.73998","source":"Crossref","is-referenced-by-count":30,"title":["Integrative analysis of scRNA-seq and scATAC-seq revealed transit-amplifying thymic epithelial cells expressing autoimmune regulator"],"prefix":"10.7554","volume":"11","author":[{"given":"Takahisa","family":"Miyao","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04mb6s476","id-type":"ROR","asserted-by":"publisher"}],"name":"Laboratory for Immune Homeostasis, RIKEN Center for Integrative 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