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The ability of thymic macrophages to engulf apoptotic thymocytes is well appreciated, but little is known about their ontogeny, maintenance, and diversity. Here, we characterized the surface phenotype and transcriptional profile of these cells and defined their expression signature. Thymic macrophages were most closely related to spleen red pulp macrophages and Kupffer cells and shared the expression of the transcription factor (TF) SpiC with these cells. Single-cell RNA sequencing (scRNA-Seq) showed that the macrophages in the adult thymus are composed of two populations distinguished by the expression of\n                    <jats:italic>Timd4<\/jats:italic>\n                    and\n                    <jats:italic>Cx3cr1<\/jats:italic>\n                    . Remarkably,\n                    <jats:italic>Timd4<\/jats:italic>\n                    <jats:sup>+<\/jats:sup>\n                    cells were located in the cortex, while\n                    <jats:italic>Cx3cr1<\/jats:italic>\n                    <jats:sup>+<\/jats:sup>\n                    macrophages were restricted to the medulla and the cortico-medullary junction. Using shield chimeras, transplantation of embryonic thymuses, and genetic fate mapping, we found that the two populations have distinct origins.\n                    <jats:italic>Timd4<\/jats:italic>\n                    <jats:sup>+<\/jats:sup>\n                    thymic macrophages are of embryonic origin, while\n                    <jats:italic>Cx3cr1<\/jats:italic>\n                    <jats:sup>+<\/jats:sup>\n                    macrophages are derived from adult hematopoietic stem cells. Aging has a profound effect on the macrophages in the thymus.\n                    <jats:italic>Timd4<\/jats:italic>\n                    <jats:sup>+<\/jats:sup>\n                    cells underwent gradual attrition, while\n                    <jats:italic>Cx3cr1<\/jats:italic>\n                    <jats:sup>+<\/jats:sup>\n                    cells slowly accumulated with age and, in older mice, were the dominant macrophage population in the thymus. Altogether, our work defines the phenotype, origin, and diversity of thymic macrophages.\n                  <\/jats:p>","DOI":"10.7554\/elife.75148","type":"journal-article","created":{"date-parts":[[2022,11,30]],"date-time":"2022-11-30T08:02:11Z","timestamp":1669795331000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.75148","source":"Crossref","is-referenced-by-count":52,"title":["Thymic macrophages consist of two populations with distinct localization and origin"],"prefix":"10.7554","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4031-4947","authenticated-orcid":true,"given":"Tyng-An","family":"Zhou","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00se2k293","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute of Microbiology and Immunology, National Yang Ming Chiao Tung University","place":["Taipei, 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