{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T14:38:38Z","timestamp":1785422318925,"version":"3.56.0"},"reference-count":30,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2022,9,12]],"date-time":"2022-09-12T00:00:00Z","timestamp":1662940800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2022,9,12]],"date-time":"2022-09-12T00:00:00Z","timestamp":1662940800000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2022,9,12]],"date-time":"2022-09-12T00:00:00Z","timestamp":1662940800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["1R35GM147704-01"],"award-info":[{"award-number":["1R35GM147704-01"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elifesciences.org"],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2022,8,8]]},"abstract":"<jats:p>\n                    The\n                    <jats:italic>Caenorhabditis elegans<\/jats:italic>\n                    adult hermaphrodite germline is surrounded by a thin tube formed by somatic sheath cells that support germ cells as they mature from the stem-like mitotic state through meiosis, gametogenesis, and ovulation. Recently, we discovered that the distal Sh1 sheath cells associate with mitotic germ cells as they exit the niche Gordon et al., 2020. Here, we report that these sheath-associated germ cells differentiate first in animals with temperature-sensitive mutations affecting germ cell state, and stem-like germ cells are maintained distal to the Sh1 boundary. We analyze several markers of the distal sheath, which is best visualized with endogenously tagged membrane proteins, as overexpressed fluorescent proteins fail to localize to distal membrane processes and can cause gonad morphology defects. However, such reagents with highly variable expression can be used to determine the relative positions of the two Sh1 cells, one of which often extends further distal than the other.\n                  <\/jats:p>","DOI":"10.7554\/elife.75497","type":"journal-article","created":{"date-parts":[[2022,8,8]],"date-time":"2022-08-08T06:15:13Z","timestamp":1659939313000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.75497","source":"Crossref","is-referenced-by-count":12,"title":["The C. elegans gonadal sheath Sh1 cells extend asymmetrically over a differentiating germ cell population in the proliferative zone"],"prefix":"10.7554","volume":"11","author":[{"given":"Xin","family":"Li","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0130frc33","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biology, The University of North Carolina at Chapel Hill","place":["Chapel Hill, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noor","family":"Singh","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0130frc33","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biology, The University of North Carolina at Chapel Hill","place":["Chapel Hill, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camille","family":"Miller","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0130frc33","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biology, The University of North Carolina at Chapel Hill","place":["Chapel Hill, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"India","family":"Washington","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0130frc33","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biology, The University of North Carolina at Chapel Hill","place":["Chapel Hill, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bintou","family":"Sosseh","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0130frc33","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biology, The University of North Carolina at Chapel Hill","place":["Chapel Hill, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0967-4020","authenticated-orcid":true,"given":"Kacy Lynn","family":"Gordon","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0130frc33","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biology, The University of North Carolina at Chapel Hill","place":["Chapel Hill, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4374","published-online":{"date-parts":[[2022,9,12]]},"reference":[{"key":"bib1","doi-asserted-by":"publisher","first-page":"2048","DOI":"10.1073\/pnas.0912704107","article-title":"Progression from a stem cell-like state to early differentiation in the C. elegans germ line","volume":"107","author":"Cinquin","year":"2010","journal-title":"PNAS"},{"key":"bib2","doi-asserted-by":"publisher","DOI":"10.1002\/0471142727.mb1420s92","article-title":"Computer control of microscopes using manager","volume":"92","author":"Edelstein","year":"2010","journal-title":"Current Protocols in Molecular Biology"},{"key":"bib3","doi-asserted-by":"publisher","DOI":"10.1895\/wormbook.1.108.1","volume-title":"The C. 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