{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:44:15Z","timestamp":1791333855582,"version":"4.3.1"},"reference-count":83,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2022,4,26]],"date-time":"2022-04-26T00:00:00Z","timestamp":1650931200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2022,4,26]],"date-time":"2022-04-26T00:00:00Z","timestamp":1650931200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2022,4,26]],"date-time":"2022-04-26T00:00:00Z","timestamp":1650931200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["1R01AI148529-02"],"award-info":[{"award-number":["1R01AI148529-02"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["IOS 2024252"],"award-info":[{"award-number":["IOS 2024252"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["1R01AI148541-02"],"award-info":[{"award-number":["1R01AI148541-02"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["5R21AG065733-02"],"award-info":[{"award-number":["5R21AG065733-02"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elifesciences.org"],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2022,4,25]]},"abstract":"<jats:p>\n                    Mosquitoes transmit numerous pathogens, but large gaps remain in our understanding of their physiology. To facilitate explorations of mosquito biology, we have created Aegypti-Atlas (\n                    <jats:ext-link ext-link-type=\"uri\" xlink:href=\"http:\/\/aegyptiatlas.buchonlab.com\/\">http:\/\/aegyptiatlas.buchonlab.com\/<\/jats:ext-link>\n                    ), an online resource hosting RNAseq profiles of Ae. aegypti body parts (head, thorax, abdomen, gut, Malpighian tubules, ovaries), gut regions (crop, proventriculus, anterior and posterior midgut, hindgut), and a gut time course of blood meal digestion. Using Aegypti-Atlas, we provide insights into regionalization of gut function, blood feeding response, and immune defenses. We find that the anterior and posterior midgut possess digestive specializations which are preserved in the blood-fed state. Blood feeding initiates the sequential induction and repression\/depletion of multiple cohorts of peptidases. With respect to defense, immune signaling components, but not recognition or effector molecules, show enrichment in ovaries. Basal expression of antimicrobial peptides is dominated by holotricin and gambicin, which are expressed in carcass and digestive tissues, respectively, in a mutually exclusive manner. In the midgut, gambicin and other effectors are almost exclusively expressed in the anterior regions, while the posterior midgut exhibits hallmarks of immune tolerance. Finally, in a cross-species comparison between Ae. aegypti and Anopheles gambiae midguts, we observe that regional digestive and immune specializations are conserved, indicating that our dataset may be broadly relevant to multiple mosquito species. We demonstrate that the expression of orthologous genes is highly correlated, with the exception of a \u2018species signature\u2019 comprising a few highly\/disparately expressed genes. With this work, we show the potential of Aegypti-Atlas to unlock a more complete understanding of mosquito biology.\n                  <\/jats:p>","DOI":"10.7554\/elife.76132","type":"journal-article","created":{"date-parts":[[2022,4,26]],"date-time":"2022-04-26T08:01:40Z","timestamp":1650960100000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.76132","source":"Crossref","is-referenced-by-count":98,"title":["A transcriptomic atlas of Aedes aegypti reveals detailed functional organization of major body parts and gut regional specializations in sugar-fed and blood-fed adult females"],"prefix":"10.7554","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7983-5104","authenticated-orcid":true,"given":"Bretta","family":"Hixson","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05bnh6r87","id-type":"ROR","asserted-by":"publisher"}],"name":"Cornell Institute of Host-Microbe Interactions and Disease, Department of Entomology, Cornell University","place":["Ithaca, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Li","family":"Bing","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05bnh6r87","id-type":"ROR","asserted-by":"publisher"}],"name":"Cornell Institute of Host-Microbe Interactions and Disease, Department of Entomology, Cornell University","place":["Ithaca, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaowei","family":"Yang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05bnh6r87","id-type":"ROR","asserted-by":"publisher"}],"name":"Cornell Institute of Host-Microbe Interactions and Disease, Department of Entomology, Cornell University","place":["Ithaca, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6642-8665","authenticated-orcid":true,"given":"Alessandro","family":"Bonfini","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05bnh6r87","id-type":"ROR","asserted-by":"publisher"}],"name":"Cornell Institute of Host-Microbe Interactions and Disease, Department of Entomology, Cornell University","place":["Ithaca, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5053-0646","authenticated-orcid":true,"given":"Peter","family":"Nagy","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05bnh6r87","id-type":"ROR","asserted-by":"publisher"}],"name":"Cornell Institute of Host-Microbe Interactions and Disease, Department of Entomology, Cornell University","place":["Ithaca, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3636-8387","authenticated-orcid":true,"given":"Nicolas","family":"Buchon","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05bnh6r87","id-type":"ROR","asserted-by":"publisher"}],"name":"Cornell Institute of Host-Microbe Interactions and Disease, Department of Entomology, Cornell University","place":["Ithaca, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4374","published-online":{"date-parts":[[2022,4,26]]},"reference":[{"key":"bib1","doi-asserted-by":"publisher","first-page":"1493","DOI":"10.1534\/g3.113.006742","article-title":"The developmental transcriptome of the mosquito Aedes aegypti, an invasive species and major arbovirus vector","volume":"3","author":"Akbari","year":"2013","journal-title":"G3: Genes, Genomes, Genetics"},{"key":"bib2","article-title":"FastQC","volume-title":"GitHub","author":"Andrews","year":"2020","unstructured":"Andrews S, Mah\u00e9 F, Yi H, Brokamp J, mareq. 2020. FastQC. GitHub. 36532ce. https:\/\/github.com\/s-andrews\/FastQC."},{"key":"bib3","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.02050","article-title":"Aedes aegypti Molecular Responses to Zika Virus: Modulation of Infection by the Toll and Jak\/Stat Immune Pathways and Virus Host Factors","volume":"8","author":"Angler\u00f3-Rodr\u00edguez","year":"2017","journal-title":"Frontiers in Microbiology"},{"key":"bib4","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-12-296","article-title":"A comprehensive gene expression atlas of sex- and tissue-specificity in the malaria vector, Anopheles gambiae","volume":"12","author":"Baker","year":"2011","journal-title":"BMC Genomics"},{"key":"bib5","doi-asserted-by":"publisher","first-page":"825","DOI":"10.1016\/0020-1790(91)90089-W","article-title":"cDNA and deduced amino acid sequence of a blood meal-induced trypsin from the mosquito, Aedes aegypti","volume":"21","author":"Barillas-Mury","year":"1991","journal-title":"Insect Biochemistry"},{"key":"bib6","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1007279","article-title":"The DH31\/CGRP enteroendocrine peptide triggers intestinal contractions favoring the elimination of opportunistic bacteria","volume":"14","author":"Benguettat","year":"2018","journal-title":"PLOS Pathogens"},{"key":"bib7","doi-asserted-by":"publisher","first-page":"190","DOI":"10.1016\/j.ibmb.2007.10.008","article-title":"Characterization of a juvenile hormone-regulated chymotrypsin-like serine protease gene in Aedes aegypti mosquito","volume":"38","author":"Bian","year":"2008","journal-title":"Insect Biochemistry and Molecular Biology"},{"key":"bib8","doi-asserted-by":"publisher","first-page":"219","DOI":"10.1146\/annurev.en.35.010190.001251","article-title":"The Midgut Ultrastructure of Hematophagous Insects","volume":"35","author":"Billingsley","year":"1990","journal-title":"Annual Review of Entomology"},{"key":"bib9","doi-asserted-by":"publisher","first-page":"865","DOI":"10.1093\/jmedent\/28.6.865","article-title":"Blood digestion in the mosquito, Anopheles stephensi Liston (Diptera: Culicidae): activity and distribution of trypsin, aminopeptidase, and alpha-glucosidase in the midgut","volume":"28","author":"Billingsley","year":"1991","journal-title":"Journal of Medical Entomology"},{"key":"bib10","article-title":"RNAseq_analysis_of_Aedes_aegypti","volume-title":"GitHub","author":"Bing","year":"2022","unstructured":"Bing X. 2022. RNAseq_analysis_of_Aedes_aegypti. GitHub. 0755da9. https:\/\/github.com\/bingdun\/RNAseq_analysis_of_Aedes_aegypti."},{"key":"bib11","doi-asserted-by":"publisher","first-page":"736","DOI":"10.1016\/j.jinsphys.2010.01.003","article-title":"Expression profiling and comparative analyses of seven midgut serine proteases from the yellow fever mosquito, Aedes aegypti","volume":"56","author":"Brackney","year":"2010","journal-title":"Journal of Insect Physiology"},{"key":"bib12","doi-asserted-by":"publisher","first-page":"200","DOI":"10.1016\/j.chom.2009.01.003","article-title":"Drosophila intestinal response to bacterial infection: activation of host defense and stem cell proliferation","volume":"5","author":"Buchon","year":"2009","journal-title":"Cell Host & Microbe"},{"key":"bib13","doi-asserted-by":"publisher","first-page":"1725","DOI":"10.1016\/j.celrep.2013.04.001","article-title":"Morphological and molecular characterization of adult midgut compartmentalization in Drosophila","volume":"3","author":"Buchon","year":"2013","journal-title":"Cell Reports"},{"key":"bib14","doi-asserted-by":"publisher","first-page":"2","DOI":"10.1016\/j.ibmb.2015.05.015","article-title":"All for one and one for all: Regionalization of the Drosophila intestine","volume":"67","author":"Buchon","year":"2015","journal-title":"Insect Biochemistry and Molecular Biology"},{"key":"bib15","doi-asserted-by":"publisher","first-page":"715","DOI":"10.1038\/ng2049","article-title":"Using FlyAtlas to identify better Drosophila melanogaster models of human disease","volume":"39","author":"Chintapalli","year":"2007","journal-title":"Nature Genetics"},{"key":"bib16","doi-asserted-by":"publisher","first-page":"729","DOI":"10.1016\/j.ibmb.2012.06.005","article-title":"Tissue-enriched expression profiles in Aedes aegypti identify hemocyte-specific transcriptome responses to infection","volume":"42","author":"Choi","year":"2012","journal-title":"Insect Biochemistry and Molecular Biology"},{"key":"bib17","doi-asserted-by":"publisher","first-page":"323","DOI":"10.1016\/j.dci.2012.10.009","article-title":"Caudal is a negative regulator of the Anopheles IMD Pathway that controls resistance to Plasmodium falciparum infection","volume":"39","author":"Clayton","year":"2013","journal-title":"Developmental & Comparative Immunology"},{"key":"bib18","doi-asserted-by":"crossref","DOI":"10.1079\/9780851993744.0000","volume-title":"The Biology of Mosquitoes Volume 1: Development, Nutrition and Reproduction","author":"Clements","year":"1992"},{"key":"bib19","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2020.103496","article-title":"Heterogeneity of midgut cells and their differential responses to blood meal ingestion by the mosquito, Aedes aegypti","volume":"127","author":"Cui","year":"2020","journal-title":"Insect Biochemistry and Molecular Biology"},{"key":"bib20","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-017-3775-6","article-title":"The midgut transcriptome of Aedes aegypti fed with saline or protein meals containing chikungunya virus reveals genes potentially involved in viral midgut escape","volume":"18","author":"Dong","year":"2017","journal-title":"BMC Genomics"},{"key":"bib21","doi-asserted-by":"publisher","DOI":"10.1002\/9780470151808.sc02f02s34","article-title":"Regional Cell Specific RNA Expression Profiling of FACS Isolated Drosophila Intestinal Cell Populations","volume":"34","author":"Dutta","year":"2015","journal-title":"Current Protocols in Stem Cell Biology"},{"key":"bib22","doi-asserted-by":"publisher","DOI":"10.1038\/srep43618","article-title":"Evaluation of reference genes at different developmental stages for quantitative real-time PCR in Aedes aegypti","volume":"7","author":"Dzaki","year":"2017","journal-title":"Scientific Reports"},{"key":"bib23","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-019-1832-y","article-title":"OrthoFinder: phylogenetic orthology inference for comparative genomics","volume":"20","author":"Emms","year":"2019","journal-title":"Genome Biology"},{"key":"bib24","doi-asserted-by":"publisher","first-page":"197","DOI":"10.1016\/0020-1790(91)90050-O","article-title":"Post-feeding induction of trypsin in the midgut of Aedes aegypti L. (Diptera: Culicidae) is separable into two cellular phases","volume":"21","author":"Felix","year":"1991","journal-title":"Insect Biochemistry"},{"key":"bib25","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2019251118","article-title":"The mosquito protein AEG12 displays both cytolytic and antiviral properties via a common lipid transfer mechanism","volume":"118","author":"Foo","year":"2021","journal-title":"PNAS"},{"key":"bib26","doi-asserted-by":"publisher","DOI":"10.1016\/j.cois.2021.11.008","article-title":"VectorBase.org updates: bioinformatic resources for invertebrate vectors of human pathogens and related organisms","volume":"50","author":"Giraldo-Calder\u00f3n","year":"2022","journal-title":"Current Opinion in Insect Science"},{"key":"bib27","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41598-018-29415-5","article-title":"The Effect of SkitoSnack, an Artificial Blood Meal Replacement, on Aedes aegypti Life History Traits and Gut Microbiota","volume":"8","author":"Gonzales","year":"2018","journal-title":"Scientific Reports"},{"key":"bib28","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1093\/jmedent\/3.1.53","article-title":"Physiological Aspects Of Digestion Of The Blood Meal By Aedes Aegypti (Linnaeus) And Culex Fatigans Wiedemann1, 2","volume":"3","author":"Gooding","year":"1966","journal-title":"Journal of Medical Entomology"},{"key":"bib29","doi-asserted-by":"publisher","first-page":"845","DOI":"10.5962\/bhl.part.82479","article-title":"Comparison between aminopeptidase and trypsin activity in blood-fed females of Aedes aegypti","volume":"89","author":"Graf","year":"1982","journal-title":"Revue Suisse de Zoologie"},{"key":"bib30","doi-asserted-by":"publisher","first-page":"451","DOI":"10.1016\/S0022-1910(98)00042-0","article-title":"A temporal profile of the endocrine control of trypsin synthesis in the yellow fever mosquito, Aedes aegypti","volume":"44","author":"Graf","year":"1998","journal-title":"Journal of Insect Physiology"},{"key":"bib31","doi-asserted-by":"publisher","first-page":"275","DOI":"10.1016\/j.actatropica.2010.04.011","article-title":"Culture-dependent and culture-independent characterization of microorganisms associated with Aedes aegypti (Diptera: Culicidae) (L.) and dynamics of bacterial colonization in the midgut","volume":"115","author":"Gusm\u00e3o","year":"2010","journal-title":"Acta Tropica"},{"key":"bib32","doi-asserted-by":"publisher","first-page":"3255","DOI":"10.1073\/pnas.72.8.3255","article-title":"The ovary as a source of alpha-ecdysone in an adult mosquito","volume":"72","author":"Hagedorn","year":"1975","journal-title":"PNAS"},{"key":"bib33","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000793","article-title":"Tsetse EP protein protects the fly midgut from trypanosome establishment","volume":"6","author":"Haines","year":"2010","journal-title":"PLOS Pathogens"},{"key":"bib34","doi-asserted-by":"publisher","first-page":"1155","DOI":"10.1016\/j.ibmb.2003.07.001","article-title":"Proventriculus (cardia) plays a crucial role in immunity in tsetse fly (Diptera: Glossinidiae","volume":"33","author":"Hao","year":"2003","journal-title":"Insect Biochemistry and Molecular Biology"},{"key":"bib35","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2021.103580","article-title":"E93 confers steroid hormone responsiveness of digestive enzymes to promote blood meal digestion in the midgut of the mosquito Aedes aegypti","volume":"134","author":"He","year":"2021","journal-title":"Insect Biochemistry and Molecular Biology"},{"key":"bib36","doi-asserted-by":"publisher","first-page":"321","DOI":"10.1007\/BF00219894","article-title":"Structure and function of midgut epithelial cells in culicidae mosquitoes (insecta, diptera","volume":"184","author":"Hecker","year":"1977","journal-title":"Cell and Tissue Research"},{"key":"bib37","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1016\/j.jinsphys.2014.11.003","article-title":"Coordinated changes in JH biosynthesis and JH hemolymph titers in Aedes aegypti mosquitoes","volume":"72","author":"Hern\u00e1ndez-Mart\u00ednez","year":"2015","journal-title":"Journal of Insect Physiology"},{"key":"bib38","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-67811-y","article-title":"Limited influence of the microbiome on the transcriptional profile of female Aedes aegypti mosquitoes","volume":"10","author":"Hyde","year":"2020","journal-title":"Scientific Reports"},{"key":"bib39","doi-asserted-by":"publisher","first-page":"903","DOI":"10.1016\/j.ibmb.2009.10.008","article-title":"Molecular genetic analysis of midgut serine proteases in Aedes aegypti mosquitoes","volume":"39","author":"Isoe","year":"2009","journal-title":"Insect Biochemistry and Molecular Biology"},{"key":"bib40","doi-asserted-by":"publisher","first-page":"68","DOI":"10.1016\/j.ibmb.2008.09.006","article-title":"Molecular analysis of the Aedes aegypti carboxypeptidase gene family","volume":"39","author":"Isoe","year":"2009","journal-title":"Insect Biochemistry and Molecular Biology"},{"key":"bib41","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-16010-4","article-title":"Accelerating invasion potential of disease vector Aedes aegypti under climate change","volume":"11","author":"Iwamura","year":"2020","journal-title":"Nature Communications"},{"key":"bib42","doi-asserted-by":"publisher","first-page":"283","DOI":"10.1016\/s0965-1748(97)00001-5","article-title":"cDNA cloning and pattern of expression of an adult, female-specific chymotrypsin from Aedes aegypti midgut","volume":"27","author":"Jiang","year":"1997","journal-title":"Insect Biochemistry and Molecular Biology"},{"key":"bib43","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.ibmb.2005.09.003","article-title":"Regulatory mechanisms of chitin biosynthesis and roles of chitin in peritrophic matrix formation in the midgut of adult Aedes aegypti","volume":"36","author":"Kato","year":"2006","journal-title":"Insect Biochemistry and Molecular Biology"},{"key":"bib44","doi-asserted-by":"publisher","first-page":"1644","DOI":"10.1126\/science.1184008","article-title":"A peroxidase\/dual oxidase system modulates midgut epithelial immunity in Anopheles gambiae","volume":"327","author":"Kumar","year":"2010","journal-title":"Science (New York, N.Y.)"},{"key":"bib45","doi-asserted-by":"publisher","first-page":"D809","DOI":"10.1093\/nar\/gkx976","article-title":"FlyAtlas 2: A new version of the Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data","volume":"46","author":"Leader","year":"2018","journal-title":"Nucleic Acids Research"},{"key":"bib46","doi-asserted-by":"publisher","first-page":"R196","DOI":"10.1016\/j.cub.2019.02.005","article-title":"Male accessory gland molecules inhibit harmonic convergence in the mosquito Aedes aegypti","volume":"29","author":"League","year":"2019","journal-title":"Current Biology"},{"key":"bib47","doi-asserted-by":"publisher","first-page":"377","DOI":"10.1146\/annurev-genet-111212-133343","article-title":"The digestive tract of Drosophila melanogaster","volume":"47","author":"Lemaitre","year":"2013","journal-title":"Annual Review of Genetics"},{"key":"bib48","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1046\/j.1365-2443.1997.1090306.x","article-title":"Transcriptional activation through interaction of MBF2 with TFIIA","volume":"2","author":"Li","year":"1997","journal-title":"Genes to Cells"},{"key":"bib49","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2017.00283","article-title":"RNA-Seq Comparison of Larval and Adult Malpighian Tubules of the Yellow Fever Mosquito Aedes aegypti Reveals Life Stage-Specific Changes in Renal Function","volume":"8","author":"Li","year":"2017","journal-title":"Frontiers in Physiology"},{"key":"bib50","doi-asserted-by":"publisher","first-page":"437","DOI":"10.1006\/dbio.2000.9836","article-title":"MBF2 is a tissue- and stage-specific coactivator that Is regulated at the step of nuclear transport in the silkworm Bombyx mori","volume":"225","author":"Liu","year":"2000","journal-title":"Developmental Biology"},{"key":"bib51","doi-asserted-by":"publisher","DOI":"10.1038\/nmicrobiol.2016.87","article-title":"Flavivirus NS1 protein in infected host sera enhances viral acquisition by mosquitoes","volume":"1","author":"Liu","year":"2016","journal-title":"Nature Microbiology"},{"key":"bib52","doi-asserted-by":"publisher","DOI":"10.1016\/j.envres.2020.109114","article-title":"Reviewing estimates of the basic reproduction number for dengue, Zika and chikungunya across global climate zones","volume":"182","author":"Liu","year":"2020","journal-title":"Environmental Research"},{"key":"bib53","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biology"},{"key":"bib54","doi-asserted-by":"publisher","first-page":"1440","DOI":"10.1073\/pnas.1424408112","article-title":"MicroRNA-8 targets the Wingless signaling pathway in the female mosquito fat body to regulate reproductive processes","volume":"112","author":"Lucas","year":"2015","journal-title":"PNAS"},{"key":"bib55","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/1471-2164-13-207","article-title":"Transcriptome of the adult female malaria mosquito vector Anopheles albimanus","volume":"13","author":"Mart\u00ednez-Barnetche","year":"2012","journal-title":"BMC Genomics"},{"key":"bib56","doi-asserted-by":"publisher","first-page":"501","DOI":"10.1038\/s41586-018-0692-z","article-title":"Improved reference genome of Aedes aegypti informs arbovirus vector control","volume":"563","author":"Matthews","year":"2018","journal-title":"Nature"},{"key":"bib57","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0005625","article-title":"Contemporary status of insecticide resistance in the major Aedes vectors of arboviruses infecting humans","volume":"11","author":"Moyes","year":"2017","journal-title":"PLOS Neglected Tropical Diseases"},{"key":"bib58","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-021-07551-z","article-title":"RNA-Seq analysis of blood meal induced gene-expression changes in Aedes aegypti ovaries","volume":"22","author":"Nag","year":"2021","journal-title":"BMC Genomics"},{"key":"bib59","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1111\/j.1365-2583.1996.tb00037.x","article-title":"Aedes aegypti midgut early trypsin is post-transcriptionally regulated by blood feeding","volume":"5","author":"Noriega","year":"1996","journal-title":"Insect Molecular Biology"},{"key":"bib60","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1046\/j.1365-2583.1997.00154.x","article-title":"Juvenile hormone controls early trypsin gene transcription in the midgut of Aedes aegypti","volume":"6","author":"Noriega","year":"1997","journal-title":"Insect Molecular Biology"},{"key":"bib61","doi-asserted-by":"publisher","first-page":"515","DOI":"10.1016\/s0022-1910(00)00145-1","article-title":"Neuroendocrine factors affecting the steady-state levels of early trypsin mRNA in Aedes aegypti","volume":"47","author":"Noriega","year":"2001","journal-title":"Journal of Insect Physiology"},{"key":"bib62","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1910(01)00165-2","article-title":"Midgut exopeptidase activities in Aedes aegypti are induced by blood feeding","volume":"48","author":"Noriega","year":"2002","journal-title":"In Journal of Insect Physiology"},{"key":"bib63","doi-asserted-by":"publisher","first-page":"1385","DOI":"10.1038\/s41564-018-0268-6","article-title":"Control of dengue virus in the midgut of Aedes aegypti by ectopic expression of the dsRNA-binding protein Loqs2","volume":"3","author":"Olmo","year":"2018","journal-title":"Nature Microbiology"},{"key":"bib64","doi-asserted-by":"publisher","first-page":"1563","DOI":"10.4269\/ajtmh.17-0866","article-title":"Mosquito-Borne Human Viral Diseases: Why Aedes aegypti?","volume":"98","author":"Powell","year":"2018","journal-title":"The American Journal of Tropical Medicine and Hygiene"},{"key":"bib65","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-12-153","article-title":"Rhythms and synchronization patterns in gene expression in the Aedes aegypti mosquito","volume":"12","author":"Ptitsyn","year":"2011","journal-title":"BMC Genomics"},{"key":"bib66","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1016\/j.pt.2010.08.004","article-title":"Pyrethroid resistance in African anopheline mosquitoes: what are the implications for malaria control?","volume":"27","author":"Ranson","year":"2011","journal-title":"Trends in Parasitology"},{"key":"bib67","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0006152","article-title":"Individual co-variation between viral RNA load and gene expression reveals novel host factors during early dengue virus infection of the Aedes aegypti midgut","volume":"11","author":"Raquin","year":"2017","journal-title":"PLOoS Neglected Tropical Diseases"},{"key":"bib68","doi-asserted-by":"publisher","first-page":"777","DOI":"10.1126\/science.1149357","article-title":"Innate immune homeostasis by the homeobox gene caudal and commensal-gut mutualism in Drosophila","volume":"319","author":"Ryu","year":"2008","journal-title":"Science (New York, N.Y.)"},{"key":"bib69","doi-asserted-by":"publisher","first-page":"1105","DOI":"10.1016\/s0965-1748(03)00124-3","article-title":"Blood meal induces global changes in midgut gene expression in the disease vector, Aedes aegypti","volume":"33","author":"Sanders","year":"2003","journal-title":"Insect Biochemistry and Molecular Biology"},{"key":"bib70","doi-asserted-by":"publisher","first-page":"947","DOI":"10.1016\/j.ibmb.2005.03.012","article-title":"Identification and characterization of a novel peritrophic matrix protein, Ae-Aper50, and the microvillar membrane protein, AEG12, from the mosquito, Aedes aegypti","volume":"35","author":"Shao","year":"2005","journal-title":"Insect Biochemistry and Molecular Biology"},{"key":"bib71","doi-asserted-by":"publisher","first-page":"867","DOI":"10.1016\/0022-1910(86)90102-2","article-title":"Juvenile hormone and juvenile hormone esterase in adult females of the mosquito Aedes aegypti","volume":"32","author":"Shapiro","year":"1986","journal-title":"Journal of Insect Physiology"},{"key":"bib72","doi-asserted-by":"publisher","first-page":"246","DOI":"10.1038\/nrm3089","article-title":"PIWI-interacting small RNAs: the vanguard of genome defence","volume":"12","author":"Siomi","year":"2011","journal-title":"Nature Reviews. Molecular Cell Biology"},{"key":"bib73","doi-asserted-by":"publisher","first-page":"488","DOI":"10.1089\/omi.2017.0073","article-title":"Mosquito-Borne Diseases and Omics: Tissue-Restricted Expression and Alternative Splicing Revealed by Transcriptome Profiling of Anopheles stephensi","volume":"21","author":"Sreenivasamurthy","year":"2017","journal-title":"Omics"},{"key":"bib74","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006847","article-title":"Comparative transcriptomics reveals CrebA as a novel regulator of infection tolerance in D. melanogaster","volume":"14","author":"Troha","year":"2018","journal-title":"PLOS Pathogens"},{"key":"bib75","doi-asserted-by":"publisher","DOI":"10.3390\/v11060513","article-title":"RNASeq Analysis of Aedes albopictus Mosquito Midguts after Chikungunya Virus Infection","volume":"11","author":"Vedururu","year":"2019","journal-title":"Viruses"},{"key":"bib76","doi-asserted-by":"publisher","first-page":"1185","DOI":"10.1016\/j.cub.2005.06.044","article-title":"Functional Genomic Analysis of Midgut Epithelial Responses in Anopheles during Plasmodium Invasion","volume":"15","author":"Vlachou","year":"2005","journal-title":"Current Biology"},{"key":"bib77","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-8-37","article-title":"Spatial and sex-specific dissection of the Anopheles gambiae midgut transcriptome","volume":"8","author":"Warr","year":"2007","journal-title":"BMC Genomics"},{"key":"bib78","doi-asserted-by":"publisher","first-page":"2211","DOI":"10.1242\/jeb.00385","article-title":"The V-type H+-ATPase in Malpighian tubules of Aedes aegypti : localization and activity","volume":"206","author":"Weng","year":"2003","journal-title":"Journal of Experimental Biology"},{"key":"bib79","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0194734","article-title":"Characterization of the adult Aedes aegypti early midgut peritrophic matrix proteome using LC-MS","volume":"13","author":"Whiten","year":"2018","journal-title":"PLOS ONE"},{"key":"bib80","article-title":"Vector-borne diseases","author":"World Health Organisation","year":"2020","unstructured":"World Health Organisation. 2020a. Vector-borne diseases. https:\/\/www.who.int\/en\/news-room\/fact-sheets\/detail\/vector-borne-diseases [Accessed July 22, 2020]."},{"key":"bib81","article-title":"Insecticide resistance","author":"World Health Organisation","year":"2020","unstructured":"World Health Organisation. 2020b. Insecticide resistance. http:\/\/www.who.int\/malaria\/areas\/vector_control\/insecticide_resistance\/en\/ [Accessed November 20, 2020]."},{"key":"bib82","doi-asserted-by":"publisher","DOI":"10.1038\/nmicrobiol.2017.20","article-title":"A Mesh-Duox pathway regulates homeostasis in the insect gut","volume":"2","author":"Xiao","year":"2017","journal-title":"Nature Microbiology"},{"key":"bib83","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1016\/j.ibmb.2016.08.003","article-title":"Determination of juvenile hormone titers by means of LC-MS\/MS\/MS and a juvenile hormone-responsive Gal4\/UAS system in Aedes aegypti mosquitoes","volume":"77","author":"Zhao","year":"2016","journal-title":"Insect Biochemistry and Molecular Biology"}],"container-title":["eLife"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/cdn.elifesciences.org\/articles\/76132\/elife-76132-v2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/cdn.elifesciences.org\/articles\/76132\/elife-76132-v2.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/elifesciences.org\/articles\/76132","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,20]],"date-time":"2023-11-20T11:47:15Z","timestamp":1700480835000},"score":1,"resource":{"primary":{"URL":"https:\/\/elifesciences.org\/articles\/76132"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,4,26]]},"references-count":83,"alternative-id":["10.7554\/eLife.76132"],"URL":"https:\/\/doi.org\/10.7554\/elife.76132","archive":["CLOCKSS"],"relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2021.12.19.473372","asserted-by":"subject"},{"id-type":"doi","id":"10.1101\/2021.12.19.473372","asserted-by":"object"}],"is-supplemented-by":[{"id-type":"accession","id":"PRJNA789580","asserted-by":"subject"}],"has-review":[{"id-type":"doi","id":"10.7554\/eLife.76132.sa1","asserted-by":"object"},{"id-type":"doi","id":"10.7554\/eLife.76132.sa0","asserted-by":"object"},{"id-type":"doi","id":"10.7554\/eLife.76132.sa2","asserted-by":"object"}]},"ISSN":["2050-084X"],"issn-type":[{"value":"2050-084X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,4,26]]},"assertion":[{"value":"single anonymised","name":"peer_review_transparency","label":"Peer review transparency","group":{"name":"peer_review_taxonomy"}},{"value":"other reviewer(s), editor","name":"peer_review_interaction","label":"Peer review interaction","group":{"name":"peer_review_taxonomy"}},{"value":"review summaries, review reports, author\/editor communication, reviewer identities reviewer opt in, editor identities","name":"peer_review_published","label":"Peer review published","group":{"name":"peer_review_taxonomy"}},{"value":"open (sign in with ORCID iD required)","name":"post_publication_commenting","label":"Post publication commenting","group":{"name":"post_publication_commenting"}}],"article-number":"e76132"}}