{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T16:10:49Z","timestamp":1790439049263,"version":"4.1.0"},"reference-count":48,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2022,7,7]],"date-time":"2022-07-07T00:00:00Z","timestamp":1657152000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2022,7,7]],"date-time":"2022-07-07T00:00:00Z","timestamp":1657152000000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2022,7,7]],"date-time":"2022-07-07T00:00:00Z","timestamp":1657152000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002808","name":"Carlsbergfondet","doi-asserted-by":"publisher","award":["CF16-0342"],"award-info":[{"award-number":["CF16-0342"]}],"id":[{"id":"10.13039\/501100002808","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003554","name":"Lundbeckfonden","doi-asserted-by":"publisher","award":["Postdoctoral fellowship"],"award-info":[{"award-number":["Postdoctoral fellowship"]}],"id":[{"id":"10.13039\/501100003554","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elifesciences.org"],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2022,6,17]]},"abstract":"<jats:p>\n                    <jats:italic>Staphylococcus epidermidis<\/jats:italic>\n                    causes some of the most hard-to-treat clinical infections by forming biofilms: Multicellular communities of bacteria encased in a protective matrix, supporting immune evasion and tolerance against antibiotics. Biofilms occur most commonly on medical implants, and a key event in implant colonization is the robust adherence to the surface, facilitated by interactions between bacterial surface proteins and host matrix components.\n                    <jats:italic>S. epidermidis<\/jats:italic>\n                    is equipped with a giant adhesive protein, extracellular matrix-binding protein (Embp), which facilitates bacterial interactions with surface-deposited, but not soluble fibronectin. The structural basis behind this selective binding process has remained obscure. Using a suite of single-cell and single-molecule analysis techniques, we show that\n                    <jats:italic>S. epidermidis<\/jats:italic>\n                    is capable of such distinction because Embp binds specifically to fibrillated fibronectin on surfaces, while ignoring globular fibronectin in solution.\n                    <jats:italic>S. epidermidis<\/jats:italic>\n                    adherence is critically dependent on multivalent interactions involving 50 fibronectin-binding repeats of Embp. This unusual, Velcro-like interaction proved critical for colonization of surfaces under high flow, making this newly identified attachment mechanism particularly relevant for colonization of intravascular devices, such as prosthetic heart valves or vascular grafts. Other biofilm-forming pathogens, such as\n                    <jats:italic>Staphylococcus aureus,<\/jats:italic>\n                    express homologs of Embp and likely deploy the same mechanism for surface colonization. Our results may open for a novel direction in efforts to combat devastating, biofilm-associated infections, as the development of implant materials that steer the conformation of adsorbed proteins is a much more manageable task than avoiding protein adsorption altogether.\n                  <\/jats:p>","DOI":"10.7554\/elife.76164","type":"journal-article","created":{"date-parts":[[2022,7,7]],"date-time":"2022-07-07T08:15:22Z","timestamp":1657181722000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.76164","source":"Crossref","is-referenced-by-count":16,"title":["The giant staphylococcal protein Embp facilitates colonization of surfaces through Velcro-like attachment to fibrillated fibronectin"],"prefix":"10.7554","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9196-9321","authenticated-orcid":true,"given":"Nasar","family":"Khan","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01aj84f44","id-type":"ROR","asserted-by":"publisher"}],"name":"Interdisciplinary Nanoscience Center (iNANO), Aarhus University","place":["Aarhus, Denmark"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0471-5452","authenticated-orcid":true,"given":"H\u00fcsn\u00fc","family":"Aslan","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01aj84f44","id-type":"ROR","asserted-by":"publisher"}],"name":"Interdisciplinary Nanoscience Center (iNANO), Aarhus University","place":["Aarhus, Denmark"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5086-4961","authenticated-orcid":true,"given":"Henning","family":"B\u00fcttner","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01zgy1s35","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute for Medical Microbiology, Virology and Hygiene, University Medical Centre Hamburg-Eppendorf","place":["Hamburg, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Holger","family":"Rohde","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01zgy1s35","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute for Medical Microbiology, Virology and Hygiene, University Medical Centre Hamburg-Eppendorf","place":["Hamburg, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thaddeus Wayne","family":"Golbek","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01aj84f44","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Chemistry, Aarhus University","place":["Aarhus C, Denmark"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven Joop","family":"Roeters","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01aj84f44","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Chemistry, Aarhus University","place":["Aarhus C, Denmark"]},{"id":[{"id":"https:\/\/ror.org\/04dkp9463","id-type":"ROR","asserted-by":"publisher"}],"name":"Van \u2018t Hoff Institute of Molecular Sciences, University of Amsterdam","place":["Amsterdam, Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sander","family":"Woutersen","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04dkp9463","id-type":"ROR","asserted-by":"publisher"}],"name":"Van \u2018t Hoff Institute of Molecular Sciences, University of Amsterdam","place":["Amsterdam, Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobias","family":"Weidner","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01aj84f44","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Chemistry, Aarhus University","place":["Aarhus C, Denmark"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6485-5134","authenticated-orcid":true,"given":"Rikke Louise","family":"Meyer","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01aj84f44","id-type":"ROR","asserted-by":"publisher"}],"name":"Interdisciplinary Nanoscience Center (iNANO), Aarhus University","place":["Aarhus, Denmark"]},{"id":[{"id":"https:\/\/ror.org\/01aj84f44","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biology, Aarhus University","place":["Aarhus C, Denmark"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4374","published-online":{"date-parts":[[2022,7,7]]},"reference":[{"key":"bib1","doi-asserted-by":"publisher","first-page":"3013","DOI":"10.1016\/s0142-9612(03)00133-9","article-title":"Staphylococcus epidermidis-fibronectin binding and its inhibition by heparin","volume":"24","author":"Arciola","year":"2003","journal-title":"Biomaterials"},{"key":"bib2","doi-asserted-by":"publisher","first-page":"397","DOI":"10.1038\/s41579-018-0019-y","article-title":"Implant infections: adhesion, biofilm formation and immune evasion","volume":"16","author":"Arciola","year":"2018","journal-title":"Nature Reviews. 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