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Here, we apply this approach to embryos. What are the first embryonic behaviors and how do they evolve as early neurodevelopment ensues? To address these questions, we present a systematic description of behavioral maturation for\n                    <jats:italic>Caenorhabditis elegans<\/jats:italic>\n                    embryos. Posture libraries were built using a genetically encoded motion capture suit imaged with light-sheet microscopy and annotated using custom tracking software. Analysis of cell trajectories, postures, and behavioral motifs revealed a stereotyped developmental progression. Early movement is dominated by flipping between dorsal and ventral coiling, which gradually slows into a period of reduced motility. Late-stage embryos exhibit sinusoidal waves of dorsoventral bends, prolonged bouts of directed motion, and a rhythmic pattern of pausing, which we designate slow wave twitch (SWT). Synaptic transmission is required for late-stage motion but not for early flipping nor the intervening inactive phase. A high-throughput behavioral assay and calcium imaging revealed that SWT is elicited by the rhythmic activity of a quiescence-promoting neuron (RIS). Similar periodic quiescent states are seen prenatally in diverse animals and may play an important role in promoting normal developmental outcomes.\n                  <\/jats:p>","DOI":"10.7554\/elife.76836","type":"journal-article","created":{"date-parts":[[2022,8,5]],"date-time":"2022-08-05T05:15:39Z","timestamp":1659676539000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.76836","source":"Crossref","is-referenced-by-count":11,"title":["Stereotyped behavioral maturation and rhythmic quiescence in C. elegans embryos"],"prefix":"10.7554","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9366-5751","authenticated-orcid":true,"given":"Evan L","family":"Ardiel","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/002pd6e78","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Molecular Biology, Massachusetts General Hospital","place":["Boston, United States"]},{"name":"Department of Neurobiology, Harvard Medical School","place":["Boston, 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