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However, proliferation follows different cell-cycle dynamics and is coupled to different functional outcomes according to T-cell subsets. Whether the mitotic machineries supporting these qualitatively distinct proliferative responses are identical remains unknown. Here, we show that disruption of the microtubule-associated protein LIS1 in mouse models leads to proliferative defects associated with a blockade of T-cell development after \u03b2-selection and of peripheral CD4+ T-cell expansion after antigen priming. In contrast, cell divisions in CD8+ T cells occurred independently of LIS1 following T-cell antigen receptor stimulation, although LIS1 was required for proliferation elicited by pharmacological activation. In thymocytes and CD4+ T cells, LIS1 deficiency did not affect signaling events leading to activation but led to an interruption of proliferation after the initial round of division and to p53-induced cell death. Proliferative defects resulted from a mitotic failure, characterized by the presence of extra-centrosomes and the formation of multipolar spindles, causing abnormal chromosomes congression during metaphase and separation during telophase. LIS1 was required to stabilize dynein\/dynactin complexes, which promote chromosome attachment to mitotic spindles and ensure centrosome integrity. Together, these results suggest that proliferative responses are supported by distinct mitotic machineries across T-cell subsets.<\/jats:p>","DOI":"10.7554\/elife.80277","type":"journal-article","created":{"date-parts":[[2022,12,15]],"date-time":"2022-12-15T06:00:52Z","timestamp":1671084052000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.80277","source":"Crossref","is-referenced-by-count":1,"title":["A selective LIS1 requirement for mitotic spindle assembly discriminates distinct T-cell division mechanisms within the T-cell lineage"],"prefix":"10.7554","volume":"11","author":[{"given":"J\u00e9r\u00e9my","family":"Argenty","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02v6kpv12","id-type":"ROR","asserted-by":"publisher"}],"name":"Toulouse Institute for Infectious and Inflammatory Diseases (Infinity), INSERM UMR1291, CNRS UMR5051, University Toulouse III","place":["Toulouse, 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