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The pathophysiology consists of a prominent inflammatory component that can be targeted to prevent cartilage degradation and structural defects. Intracellular metabolism has emerged as a culprit of the inflammatory response in chondrocytes, with both processes co-regulating each other. The role of glutamine metabolism in chondrocytes, especially in the context of inflammation, lacks a thorough understanding and is the focus of this work. We display that mouse chondrocytes utilize glutamine for energy production and anabolic processes. Furthermore, we show that glutamine deprivation itself causes metabolic reprogramming and decreases the inflammatory response of chondrocytes through inhibition of NF-\u03baB activity. Finally, we display that glutamine deprivation promotes autophagy and that ammonia is an inhibitor of autophagy. Overall, we identify a relationship between glutamine metabolism and inflammatory signaling and display the need for increased study of chondrocyte metabolic systems.<\/jats:p>","DOI":"10.7554\/elife.80725","type":"journal-article","created":{"date-parts":[[2022,8,2]],"date-time":"2022-08-02T08:08:10Z","timestamp":1659427690000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.80725","source":"Crossref","is-referenced-by-count":40,"title":["Glutamine metabolism modulates chondrocyte inflammatory response"],"prefix":"10.7554","volume":"11","author":[{"given":"Manoj","family":"Arra","sequence":"first","affiliation":[{"name":"Department of Orthopedic Surgery, Washington University School of Medicine","place":["St Louis, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaurav","family":"Swarnkar","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, Washington University School of Medicine","place":["St Louis, United 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